Research Publications from Peer-Reviewed Journals

Mon, 09/30/2019 - 17:01
Author(s): Vinay Kumar, S.K. DwivediAbstractIn the present study we are investigating the Cr(VI) reduction potential of a multi-metal tolerant fungus (isolate CR700); isolated from electroplating wastewater. Based on the ITS region sequencing, the isolate was identified as Trichoderma lixii isolate CR700 and able to tolerate As(2000 mg/L), Ni(1500 mg/L), Zn(1200 mg/L), Cu(1200 mg/L), Cr(1000 mg/L...
Mon, 09/30/2019 - 17:00
Author(s): Masafumi Amano, Noriko Amiya, Minami Takaoka, Haruka Sato, Tomohiro Takatani, Osamu Arakawa, Yoshitaka SakakuraAbstractWe tested whether tetrodotoxin (TTX) functions as a stress relieving substance in puffer fish. We orally administered TTX to the juveniles of hatchery-reared non-toxic tiger puffer Takifugu rubripes and measured the effects of TTX on brain corticotropin-releasing...
Mon, 09/30/2019 - 17:00
LWT
Author(s): Alonzo A. Gabriel, Katrina Moira D. Melo, Juan Carlos D. MichelenaAbstractThis study was conducted to determine the efficacy of UV-C irradiation for safety and quality of dried bay leaves. Cocktails of microorganisms of the same species were prepared and inoculated onto the leaves, which were thereafter subjected to UV-C irradiation. Results showed that all tested organisms, which...
Mon, 09/30/2019 - 16:54
ABSTRACT To visualize the personalized distributions of pathogens and chemical environments, including microbial metabolites, pharmaceuticals, and their metabolic products, within and between human lungs afflicted with cystic fibrosis (CF), we generated three-dimensional (3D) microbiome and metabolome maps of six explanted lungs from three cystic fibrosis patients. These 3D spatial maps revealed...
Mon, 09/30/2019 - 16:53
ABSTRACT The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) poses a considerable risk for public health. The gene for Klebsiella pneumoniae carbapenemase-2 (KPC-2) has been reported in many countries worldwide, and KPC-2-producing strains are mainly of human origin. In this study, we identified two novel hybrid plasmids that carry either blaKPC-2 or the fosfomycin resistance gene...
Mon, 09/30/2019 - 16:53
ABSTRACT The opportunistic pathogenic fungus Candida albicans can cause devastating infections in immunocompromised patients. Its ability to undergo a morphogenetic transition from yeast to filamentous forms allows it to penetrate tissues and damage tissues, and the expression of genes associated with a number of pathogenetic mechanisms is also coordinately regulated with the yeast-to-hypha...
Mon, 09/30/2019 - 16:53
ABSTRACT The polyketide-derived secondary metabolite ascochitine is produced by species in the Didymellaceae family, including but not restricted to Ascochyta species pathogens of cool-season food legumes. Ascochitine is structurally similar to the well-known mycotoxin citrinin and exhibits broad-spectrum phytotoxicity and antimicrobial activities. Here, we identified a polyketide synthase (PKS)...
Mon, 09/30/2019 - 16:53
ABSTRACT Enteric viruses in the Caliciviridae family cause acute gastroenteritis in humans and animals, but the cellular processes needed for virus replication and disease remain unknown. A common strategy among enteric viruses, including rotaviruses and enteroviruses, is to encode a viral ion channel (i.e., viroporin) that is targeted to the endoplasmic reticulum (ER) and disrupts host calcium (...
Mon, 09/30/2019 - 16:53
ABSTRACT The continuous emergence of carbapenemase-producing Enterobacteriaceae (CPE) presents a great public health challenge. Mitigation of CPE spread in the environment is crucial, particularly from a One Health perspective. Here we describe the isolation of CPE strain SNI47 from influent water of a sewage treatment plant in Japan. SNI47 was identified as Klebsiella quasipneumoniae subsp....
Mon, 09/30/2019 - 16:53
Salmonella enterica is a Gram-negative human pathogen widely known to cause food poisoning. Here, the genome of S. enterica phage Shelanagig is described. Its 42,541-bp genome codes for 68 proteins, for which 33 were assigned a predicted function. Shelanagig shares high similarity at the protein level with other Salmonella phages.
Mon, 09/30/2019 - 16:53
Acinetobacter species are important in the emergence and spread of antimicrobial resistance (AMR), which threatens human and animal health worldwide. Here, we present the draft genome sequences of three Acinetobacter species strains (RF14B, RF15A, and RF15B) isolated from pig feces and the floor of a pig hospital pen in Ireland.
Mon, 09/30/2019 - 16:53
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subinhibitory concentrations of aminoglycoside is altered. One gene identified, VC1636, encodes a putative DNA/RNA helicase, recently named RadD in Escherichia coli. Here we combined extensive genetic characterization and high-throughput approaches in order to identify partners and molecular mechanisms...
Mon, 09/30/2019 - 16:53
ABSTRACT Stenotrophomonas maltophilia is a Gram-negative, nonfermenting, environmental bacillus that is an important cause of nosocomial infections, primarily associated with the respiratory tract in the immunocompromised population. Aiming to understand the population structure, microbiological characteristics and impact of allelic variation on β-lactamase structure and function, we...
Mon, 09/30/2019 - 16:53
ABSTRACT BolA family proteins are conserved in Gram-negative bacteria and many eukaryotes. While diverse cellular phenotypes have been linked to this protein family, the molecular pathways through which these proteins mediate their effects are not well described. Here, we investigated the roles of BolA family proteins in Vibrio cholerae, the cholera pathogen. Like Escherichia coli, V. cholerae...
Mon, 09/30/2019 - 16:53
ABSTRACT Mannitol-1-phosphate dehydrogenase (M1PDH) is a key enzyme in Staphylococcus aureus mannitol metabolism, but its roles in pathophysiological settings have not been established. We performed comprehensive structure-function analysis of M1PDH from S. aureus USA300, a strain of community-associated methicillin-resistant S. aureus, to evaluate its roles in cell viability and virulence under...
Mon, 09/30/2019 - 16:53
ABSTRACT Subversion of endoplasmic reticulum (ER) function is a feature shared by multiple intracellular bacteria and viruses, and in many cases this disruption of cellular function activates pathways of the unfolded protein response (UPR). In the case of infection with Brucella abortus, the etiologic agent of brucellosis, the unfolded protein response in the infected placenta contributes to...
Mon, 09/30/2019 - 16:53
ABSTRACT Chronic wasting disease (CWD) is a prion-related transmissible spongiform encephalopathy of cervids, including deer, elk, reindeer, sika deer, and moose. CWD has been confirmed in at least 26 U.S. states, three Canadian provinces, South Korea, Finland, Norway, and Sweden, with a notable increase in the past 5 years. The continued geographic spread of this disease increases the frequency...
Mon, 09/30/2019 - 16:53
ABSTRACT The filoviruses are etiological agents of life-threatening hemorrhagic fever with high mortality rate and risk of potential outbreak. Among members of this family, the Ebola (EBOV), Sudan (SUDV), and Marburg (MARV) viruses are considered the most pathogenic for humans. The ebolavirus nucleoprotein (NP) is the most abundant protein in infected cells and is essential for viral...
Mon, 09/30/2019 - 16:53
ABSTRACT The stator units of the flagellum supply power to the flagellar motor via ion transport across the cytoplasmic membrane and generate torque on the rotor for rotation. Flagellar motors across bacterial species have evolved adaptations that impact and enhance stator function to meet the demands of each species, including producing stator units using different fuel types or various stator...
Mon, 09/30/2019 - 16:53
ABSTRACT Rod-shaped bacteria have two modes of peptidoglycan synthesis: lateral synthesis and synthesis at the cell division site. These two processes are controlled by two macromolecular protein complexes, the elongasome and divisome. Recently, it has been shown that the Bacillus subtilis RodA protein, which forms part of the elongasome, has peptidoglycan glycosyltransferase activity. The cell...
Mon, 09/30/2019 - 16:53
ABSTRACT The pathogenic bacterium Vibrio vulnificus exhibits the ability to form biofilm, for which initiation is dependent upon swimming motility by virtue of a polar flagellum. The filament of its flagellum is composed of multiple flagellin subunits, FlaA, -B, -C, and -D. In V. vulnificus genomes, however, open reading frames (ORFs) annotated by FlaE and -F are also present. Although neither...
Mon, 09/30/2019 - 16:53
ABSTRACT Here we exploit the natural properties of a synthetic nanoparticle (NP) scaffold as a subunit vaccine against enterohemorrhagic Escherichia coli (EHEC). Two EHEC-specific immunogenic antigens, namely, LomW and EscC, either alone or in combination, were covalently linked on the surface of gold nanoparticles (AuNPs) and used to immunize mice prior to challenge with EHEC O157:H7 strain 86-...
Mon, 09/30/2019 - 16:53
ABSTRACT Enteropathogenic Escherichia coli and enterohemorrhagic E. coli (EPEC and EHEC, respectively) are extracellular pathogens that reorganize the host cell cytoskeleton to form "actin pedestals" beneath the tightly adherent bacteria, a critical step in pathogenesis. EPEC and EHEC inject effector proteins that manipulate host cell signaling cascades to trigger pedestal assembly. One such...
Mon, 09/30/2019 - 16:53
ABSTRACT We found a novel role of Myo5, a type I myosin (myosin-I), and its fortuitous association with d-amino acid utilization in Cryptococcus gattii. Myo5 colocalized with actin cortical patches and was required for endocytosis. Interestingly, the myo5 mutant accumulated high levels of d-proline and d-alanine which caused toxicity in C. gattii cells. The myo5 mutant also accumulated a large...
Mon, 09/30/2019 - 03:22
Author(s): Amanda L. Dragan, Daniel E. VothAbstractCoxiella burnetii is an intracellular bacterium that causes acute and chronic Q fever. This unique pathogen has been historically challenging to study due to obstacles in genetically manipulating the organism and the inability of small animal models to fully mimic human Q fever. Here, we review the current state of C. burnetii research,...
Mon, 09/30/2019 - 03:21
Author(s): Yuan Luo, Yun-Ni Zhang, Han Zhang, Hong-Bo Lv, Mei-Ling Zhang, Li-Qiao Chen, Zhen-Yu DuAbstractPeroxisome proliferator-activated receptor α (PPARα) plays critical physiological roles in energy metabolism, antioxidation and immunity of mammals, however, these functions have not been fully understood in fish. In the present study, Nile tilapia (Oreochromis niloticus) were fed with...
Mon, 09/30/2019 - 03:20
Author(s): Khamis Youssef, Ahmed HussienAbstractThe effect of four salt solutions; sodium metabisulfite (SM), potassium sorbate (PS), potassium carbonate (PC) and sodium chloride (SC) as electrolyte to generate alkaline (alEW) and acidic (acEW) electrolysed water was assessed in vitro and ex vivo under artificial and natural infection against Penicillium digitatum and P. italicum, the causal...
Mon, 09/30/2019 - 03:20
Author(s): Chi Cao, Hua Li, Michael G.K. Jones, Stephen J. WylieAbstractDetermining roles of mycoviruses in fungal biology is complicated, especially when fungi are co-infected with multiple viruses. Genetically identical (isogenic) fungal lines that are infected by and uninfected by viruses must be created and compared. Here, we study an isolate of Ceratobasidium sp., a fungus isolated from...
Sun, 09/29/2019 - 03:21
Author(s): Yu-Xuan Zhang, Jin-Xing Wang, Xian-Wei WangAbstractWhey acidic protein domain (WAPD) is a usual motif in crustaceans, and is found mainly in the immune-related proteins. In the present study, a protein containing three tandem WAPDs was identified in red swamp crayfish Procambarus clarkii and designated as PcTWD. This is the first report of a protein of such domain architecture in...
Sun, 09/29/2019 - 03:21
Author(s): Yulong Gong, Fan Yang, Junpeng Hu, Cui Liu, Haokun Liu, Dong Han, Junyan Jin, Yunxia Yang, Xiaoming Zhu, Jianhua Yi, Shouqi XieAbstractA 56-day growth trial was conducted to investigate the effects of dietary yeast hydrolysate on the growth performance, antioxidation, immune response and resistance against Aeromonas hydrophila in largemouth bass. Four experimental diets were prepared...
Sun, 09/29/2019 - 03:21
Author(s): Chao Peng, Dongchang Xie, Chao Zhao, Haidong Xu, Sigang Fan, Lulu Yan, Pengfei Wang, Lihua QiuAbstractAkirin, which are members of the NF-κB signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. In the present study, the Akirin gene from Penaeus monodon was identified from a transcriptome database and designated as PmAkirin. The complete...
Sun, 09/29/2019 - 03:21
Author(s): Hengwei Deng, Wenfeng Zhang, Jingjing Li, Jinling Li, Lei Hu, Wenyan Yan, Shanshan Liu, Jianguo He, Shaoping WengAbstractA STAT gene from Scylla paramamosain, named SpSTAT, was cloned and characterized. The full length of SpSTAT mRNA contains a 5′untranslated region (UTR) of 238 bp, an open reading frame (ORF) of 2388 bp and a 3′ UTR of 326 bp. The SpSTAT protein contains four...
Sun, 09/29/2019 - 03:21
Author(s): Qian Feng, Yueqian Huang, Defu Yao, Chunhua Zhu, Shengkang Li, Hongyu Ma, Jude Juventus Aweya, Yueling ZhangAbstractProtein kinase CK2 (CK2) is a ubiquitous serine/threonine kinase with multiple cellular functions in vertebrates including apoptosis, differentiation, proliferation, survival, tumorigenesis, signal transduction, immune regulation and inflammation. In the current study,...
Sun, 09/29/2019 - 03:21
Author(s): Xiaoxu Liu, Weiye Li, Lihua Jiang, Zhenming Lü, Minhai Liu, Li Gong, Bingjian Liu, Liqin Liu, Xiaolong YinAbstractLong non-coding RNA refers to an RNA transcript of a non-coding protein with a sequence length greater than 200 bp. More and more reports indicated that lncRNA was involved in the regulation of gene expression as a signalling molecule, an inducing molecule, a leader...
Sun, 09/29/2019 - 03:21
Author(s): S.L. Edirisinghe, S.H.S. Dananjaya, Chamilani Nikapitiya, T.D. Liyanage, Kyoung-Ah Lee, Chulhong Oh, Do-Hyung Kang, Mahanama De ZoysaAbstractIn this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative...
Sun, 09/29/2019 - 03:21
Author(s): Ruixia Wang, Xiucai Hu, Aijun Lü, Rongrong Liu, Jingfeng Sun, Yeong Yik Sung, Yajiao SongAbstractSkin plays an important role in the innate immune responses of fish, particularly towards bacterial infection. To understand the molecular mechanism of mucosal immunity of fish during bacterial challenge, a de novo transcriptome assembly of crucian carp Carassius auratus skin upon Aeromonas...
Sun, 09/29/2019 - 03:20
Author(s): Gudina Terefe Tucho, Thuat OkothAbstractThe purpose of this study is to assess the quantity and characteristics of food wastes and human excreta generated from Jimma University with the food-energy-sanitation nexus approach. The focus of this study is on food wastes and human excreta generated by regular undergraduate students living and feeding in the university. The results of the...
Sun, 09/29/2019 - 03:20
Author(s): Han Yu, Dexing Dou, Xi Zhang, Linus Zhang, Heng Dong, Hongbing YuAbstractThe removal and degradation of refractory antibiotics in saline water by electrochemical and photo-electrochemical catalysis has emerged as a worldwide research hotspot. Aiming to improve degradation performance, most of the concerned studies focus on new sophisticated materials and modification methods on the...
Sun, 09/29/2019 - 03:20
Author(s): Masoud Khanpae, Ezatollah Karami, Hamideh Maleksaeidi, Marzieh KeshavarzAbstractIn the context of climate variability and change, application of treated wastewater for irrigation is an appropriate approach to cope with the water scarcity in arid and semi-arid regions. Farmers will adopt treated wastewater if they perceive it as an economically beneficial, socially acceptable,...
Sun, 09/29/2019 - 03:20
Author(s): Oisik Das, Nam Kyeun Kim, Mikael S. Hedenqvist, Debes Bhattacharyya, Eva Johansson, Qiang Xu, Shima HolderAbstractThe aim of the study was to impart fire retardancy in wheat gluten polymer through naturally-occurring additives such as lanosol. The fire properties of lanosol were compared with two other conventional brominated fire retardants (Tetrabromobisphenol A and...
Sun, 09/29/2019 - 03:20
Author(s): Xueke Li, Qiaoying Ming, Rui Cai, Tianli Yue, Yahong Yuan, Zhenpeng Gao, Zhouli WangAbstractThe pollution of heavy metals in fruit juice is still increasing and becoming a significant food safety issue worldwide nowadays. In this work, twelve strains of lactic acid bacteria (LAB) were evaluated for the biomass and their capability to adsorb Cd2+ and Pb2+. One strain of LAB capable of...
Sun, 09/29/2019 - 03:20
Author(s): Tanya Christidis, Matt Hurst, Wallis Rudnick, Katarina D.M. Pintar, Frank PollariAbstractSalmonella is one of the leading causes of gastro-enteric disease in Canada, second only to Campylobacter. The mean exposure of Salmonella measured in cells ingested per person per day, from 15 transmission routes, including foodborne, animal contact and waterborne routes, was estimated for Canada...
Sun, 09/29/2019 - 03:20
Author(s): Sulaiman F. Aljasir, Catherine Gensler, Lang Sun, Dennis J. D'AmicoAbstractCheeses made from unpasteurized milk are preferred by many consumers for their complex flavors and aromas among other attributes. However, the potential presence and survival of foodborne pathogens remain a concern. There is, therefore, a need for developing practical and science-based preventive strategies to...
Sun, 09/29/2019 - 03:20
Author(s): Arthur Vinicius Sousa Silva, Lilian D. Moura Torquato, Glauber CruzAbstractThe replacement of fossil fuels by renewable sources has been discussed globally, because fossil fuels account for a large portion of the pollutant emissions into the atmosphere. Several cities along the Brazilian coast produce a variety of fish types, generating a large amount of waste, including viscera and...

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