Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Wed, 11/11/2020 - 10:02
ABSTRACT RpoN, an alternative sigma factor commonly known as 54, is implicated in persistent stages of Yersinia pseudotuberculosis infections in which genes associated with this regulator are upregulated. We here combined phenotypic and genomic assays to provide insight into its role and function in this pathogen. RpoN was found essential for Y. pseudotuberculosis virulence in mice, and in vitro...
Wed, 11/11/2020 - 10:02
ABSTRACT Fermented foods have been the focus of ever greater interest as a consequence of purported health benefits. Indeed, it has been suggested that consumption of these foods helps to address the negative consequences of "industrialization" of the human gut microbiota in Western society. However, as the mechanisms via which the microbes in fermented foods improve health are not understood, it...
Wed, 11/11/2020 - 10:02
ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) infections, high in morbidity and mortality, pose serious clinical challenges due to limited treatment options. A previous CRE surveillance study on 1,507 patients from 43 hospitals in Osaka, Japan, revealed that 12% of patients carried CRE and that 95% of the CRE isolates were IMP-type carbapenemase producers. Here, the mechanisms for this...
Wed, 11/11/2020 - 10:01
Households provide a habitat for bacteria originating from humans, animals, foods, contaminated clothes, or other sources. Thus, bacteria carrying antibiotic resistance genes (ARGs) may be introduced via household members, animals, or the water supply from external habitats into private households and vice versa. Since data on antibiotic resistance (ABR) in the domestic environment are limited,...
Wed, 11/11/2020 - 10:01
This study aimed to characterize in silico enterotoxigenic Escherichia coli F4- and F18-positive isolates (n = 90) causing swine postweaning diarrhea, including pathogenic potential, phylogenetic relationship, antimicrobial and biocide resistance, prophage content, and metal tolerance rates. F4 strains belonged mostly to the O149 and O6 serogroups and ST100 and ST48 sequence types (STs). F18...
Wed, 11/11/2020 - 10:01
Microbial complexity and contamination levels in food processing plants heavily impact the final product fate and are mainly controlled by proper environmental cleaning and sanitizing. Among the emerging disinfection technologies, ozonation is considered an effective strategy to improve the ordinary cleaning and sanitizing of slaughterhouses. However, its effects on contamination levels and...
Wed, 11/11/2020 - 10:01
Cyclospora cayetanensis is a protozoan parasite that causes foodborne and waterborne diarrheal illness outbreaks worldwide. Most of these outbreaks are associated with the consumption of fresh produce. Sensitive and specific methods to detect C. cayetanensis in agricultural water are needed to identify the parasite in agricultural water used to irrigate crops that have been implicated in...
Wed, 11/11/2020 - 10:01
Oyster and seawater samples were collected from five sites in the Chesapeake Bay, MD, and three sites in the Delaware Bay, DE, from May to October 2016 and 2017. Abundances and detection frequencies for total and pathogenic Vibrio parahaemolyticus and Vibrio vulnificus were compared using the standard most-probable-number–PCR (MPN-PCR) assay and a direct-plating (DP) method on CHROMagar...
Wed, 11/11/2020 - 08:02
We evaluated the prevalence of Brucella canis seropositivity in a convenience sample of dogs from commercial breeding kennels in Ontario, Canada. Overall, 127/1,080 (11.8%) dogs from 23/63 (37%) kennels were seropositive. The prevalence of positive dogs within kennels with >1 positive dog ranged from 3.9% to 100% (median 33%).
Wed, 11/11/2020 - 08:02
A hitherto unknown composition is highlighted in the red and black inks preserved on ancient Egyptian papyri from the Roman period (circa 100 to 200 CE). Synchrotron-based macro–X-ray fluorescence (XRF) mapping brings to light the presence of iron (Fe) and lead (Pb) compounds in the majority of the red inks...
Wed, 11/11/2020 - 06:12
This study aimed to investigate the inactivation effect of 150 KeV low-energy X-ray on Salmonella Typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes that were inoculated in dry cardamom. The D10 value for E. coli O157:H7 was 71.43 Gy and the tR values for S. Typhimurium, L. monocytogenes, and S. aureus were 53.57, 87.74, and 114.64 Gy, respectively. The...
Wed, 11/11/2020 - 06:11
Peanuts in China are heavily contaminated with aflatoxin, which pose a threaten to human health. To compare the dietary exposure risk of aflatoxins (AFT) in peanuts and peanut oil in different areas of China, the spatial distribution of AFT contamination levels in peanuts and peanut oil from different areas was analyzed. The dietary exposure was calculated by simple distributed risk assessment...
Wed, 11/11/2020 - 06:09
Shiga toxin–producing E. coli (STEC) and enterotoxigenic E. coli (ETEC) are the main agents of swine colibacillosis, an infectious disease which implies important economic losses. We characterized here 186 diarrheagenic E. coli from Spanish industrial pig farms (2005-2017) to know which clones were involved in this syndrome, and the rates of antibiotic resistance. The PCR based on pathotype-...
Wed, 11/11/2020 - 06:07
Acrylamide (ACR) is generated during thermal processing of carbohydrate-rich foods at high temperature and can directly enter the body through ingestion, inhalation and skin contact. The toxicity of ACR has been widely studied. The main results of these studies show that exposure to ACR can cause neurotoxicity in both animals and humans, and show reproductive toxicity and carcinogenicity in...
Wed, 11/11/2020 - 02:02
Abstract Antimicrobial peptides (AMPs) are recognized as promising safe alternatives to antibiotics for its low drug-resistance. Brevilaterin B, a newly discovered antimicrobial lipopeptide produced by Brevibacillus laterosporus S62-9, exhibits efficient antibacterial activity on Listeria monocytogenes with a minimum inhibitory concentration of 1 μg mL−1. The present research aimed to...
Wed, 11/11/2020 - 02:02
Abstract Increasing prevalence of multidrug-resistant untreatable infections has prompted researchers to trial alternative treatments such as a substitute for traditional antibiotics. This study endeavored to elucidate the antibacterial mechanism(s) of this isoflavone, via analysis of relationship between genistein and Escherichia coli. Furthermore, this investigation analyzed whether genistein...
Wed, 11/11/2020 - 02:01
Vibrio cholerae is an important food-borne pathogenic bacterium that causes human disease, resulting in economic losses worldwide. Rapid diagnosis of cholera infection is important to avoid potential spread of disease. In this study, recombinase-aided amplification (RAA) primers labeled with biotin and fluorescein isothiocyanate (FITC) at 5′ end were prepared for the ctxA pathogenic gene of V....
Tue, 11/10/2020 - 22:11
In the present study, the efficiency of sodium acetate (2.5% wt/vol), lactic acid (2% vol/vol), citric acid (2% vol/vol), calcium chloride (3% wt/vol), Nisin (0.04 g/L), Lactic acid (2% vol/vol), and nisin (0.04 g/L), combination solutions as additional hurdle to refrigeration (4°C) of tilapia was evaluated. The solutions were applied as surface coating by dipping method. The effects of treatment...
Tue, 11/10/2020 - 22:11
Shanghai smoked fish (SSF) was first prepared in this work. Volatile flavor compounds of SSF were identified via Maillard reaction. Pyrazines and furans could modify the flavor of SSF. Glucose is beneficial to enhance the aroma of SSF. Abstract In this work, the effects of substrates on volatile flavor compounds of Shanghai smoked fish (SSF) from grass carp was investigated by head space‐solid‐...
Tue, 11/10/2020 - 18:03
It is well-documented that lead (Pb) toxicity can affect almost all systems in living organisms. It can induce selective autophagy of mitochondria (mitophagy) by triggering reactive oxygen species production. Emerging evidence has suggested that Pb-induced autophagy can also be activated by the endoplasmic reticulum (ER) stress pathway. However, the interplay between ER stress and mitophagy...
Tue, 11/10/2020 - 16:00
Extracellular vesicles are encapsulated lipid nanoparticles secreted by a variety of cell types in living organisms. They are known to carry proteins, metabolites, nucleic acids, and lipids as their cargoes and are important mediators of intercellular communication. The role of extracellular vesicles in chronic liver disease has been reported. Chronic liver disease such as viral hepatitis...
Tue, 11/10/2020 - 16:00
A novel strain of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) disease (COVID-19) has been recently identified as an infectious disease affecting the respiratory system of humans. This disease is caused by SARS-CoV-2 that was identified in Chinese patients having severe pneumonia and flu-like symptoms. COVID-19 is a contagious disease that spreads rapidly via droplet particles...
Tue, 11/10/2020 - 08:03
Multidrug-resistant Salmonella enterica subspecies enterica 4,[5],12:i:- sequence type 34 represents a worldwide public health risk. To determine its origin in the United States, we reconstructed a time-scaled phylogeny with a discrete trait geospatial model. The clone in the United States was introduced from Europe on multiple occasions in the early 2000s.
Tue, 11/10/2020 - 06:18
Application of synthetic preservatives to control the contamination of stored food commodities with aflatoxin B1 causing considerable loss in nutritional value is a major challenge. However, employment of essential oils for protecting food commodities is much limited due to high volatility, and increased susceptibility to oxidation. Therefore, objective of the present investigation was...
Tue, 11/10/2020 - 04:02
To mitigate the risk of erosion and nutrient runoff, reduced tillage has become more prevalent in Norway. Within within recent decades, there have been some years with relatively high occurrence of Fusarium head blight and mycotoxins in Norwegian cereal grain. This is thought to have been caused by an increased inoculum potential (IP) of Fusarium spp. due to larger amount of crop residues...
Tue, 11/10/2020 - 04:02
Grains and feed are severely contaminated by deoxynivalenol (DON) globally, threatening both human and animal health. Research on bio-degradation of DON, in general, is gaining attention. The aim of this research was to estimate the effect of Clostridium sp. WJ06 as a microbiological detoxification of DON based on the expression and distribution of proliferating cell nuclear antigen (PCNA) as...
Tue, 11/10/2020 - 04:02
Mycotoxins are one of the most common types of chemical hazards related to edible oils. Although the refining process can remove such contaminations, they may still be present in the final oils due to defects during the refining steps. In addition, most oils produced in local manufactories are not refined and as such may be contaminated with mycotoxins. However, the effect of various cooking...
Mon, 11/09/2020 - 22:14
The objective of this systematic review and meta‐analysis was to summarize the available knowledge on the seroprevalence of T. gondii in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in Europe. A computerized literature search of electronic databases (PubMed and CAB abstracts) was performed along with hand searches of library resources for relevant papers, books, abstracts and...
Mon, 11/09/2020 - 22:13
The role of dietary lemon peel (LM) and/or Bacillus licheniformis (BL) on the growth, immunity, and resistance against Aeromonas hydrophila in common carp, Cyprinus carpio was investigated in this study. LM and BL were included in diets at 0% (T0), 108 CFU/g BL (T1), 1.5% LM and 108 CFU/g BL (T2), and 3% LM and 108 CFU/g BL (T3). Fish fed with T1, T2, or T3 had higher weight gain, specific growth...
Mon, 11/09/2020 - 22:13
A novel, label‐free, bidirectional lateral flow immunoassay strip that detected E. coli O157:H7 in food was developed, and it has good specificity, sensitivity, stability, and practicality. The low‐cost and low‐difficulty development of the strip rapid detection technology, which requires neither nano‐labeling materials nor heavily relies on antibodies, may be easier to realize commercialization...
Mon, 11/09/2020 - 22:12
The present study aimed to examine the efficiency of ethanolic extract of Capsicum annuum L (CAE) in improving the shelf‐life and quality of fresh‐cut C. annuum (FCCa). The uniformly sliced FCCa were prewashed with sodium hypochlorite solution (NaOCl) and then treated with 10% of CAE. The food‐borne pathogens of Listeria monocytogenes and Salmonella enterica were patched on the surface of FCCa...
Mon, 11/09/2020 - 22:12
In this study, two near neutral pH electrolysed water (NEW1 produced by redirecting of the catholyte solution back to the anode chamber and NEW2 produced by using NaCl and NaHCO3 as electrolyte) and control (NEW0, produced by commercial unit) were evaluated for their stability during 75 h storageat 7°C. The physicochemical properties, bactericidal efficiencies and sanitising effects on organic...
Mon, 11/09/2020 - 22:12
The current research was performed to assess the fungal contamination in oils extracted from wild almonds (Alok) during 60 days of storage. To assess the influence of storage temperature on fungal growth and aflatoxins production, three batches of wild almond nuts were kept at 25, 30, and 35 °C for 60 days, and extracted oils from nuts were evaluated at six periods of intervals. Storage at 25 °C...
Mon, 11/09/2020 - 22:12
Peas are highly seasonal and perishable in nature, thereby requiring suitable preservation methods to be available throughout the year. Osmotic drying is one of the suitable methods for preserving perishable and semi‐perishable produce. Osmotic drying of green peas was carried out in ternary solutions of water, sodium chloride (NaCl), and sucrose having different solution concentrations and...
Mon, 11/09/2020 - 22:12
The moisture sorption isotherms for oven dried mango were constructed gravimetrically at three different temperatures of 25°C, 30°C, and 35°C, and relative humidity ranging 8.3%–97.0%. The Guggenheim–Anderson–de Boer model was used to fit sorption data and constant equations determined using nonlinear regression analysis. The isotherms had J‐shaped curve that represents type III isotherms...
Mon, 11/09/2020 - 18:02
Novel urinary protein biomarkers have recently been identified and qualified in rats for the early detection of renal injury in drug development studies. However, there are few reports on the utility of these renal biomarkers in mice, another important and widely used preclinical animal species for drug development studies. The purpose of this study was to assess the value of these recently...
Mon, 11/09/2020 - 08:02
M. de Lange et al. We examined Coxiella burnetii seroconversion rates by measuring C. burnetii IgG among 2 cohorts of veterinary students. During follow-up of 118 seronegative veterinary students, 23 students seroconverted. Although the clinical importance of the presence of antibodies is unknown, veterinary students should be informed about the potential risks for Q fever.
Mon, 11/09/2020 - 06:10
Author(s): J.M. Choi, E. Camfield, A. Bowman, K. Rajan, N. Labbé, K.D. Gwinn, B.H. Ownley, N. Moustaid-Moussa, D.H. D'Souza Recurring outbreaks linked to Escherichia coli O157:H7-contaminated lettuce and Salmonella enterica-contaminated sprouts highlight the need for improved food safety measures. The aim of this study was to determine the ability of a bio-based antimicrobial extract prepared...
Mon, 11/09/2020 - 02:01
A magnetic dispersive solid-phase microextraction procedure (MDμ-SPE) was developed to extract organophosphorus pesticides (OPP), including ethion, chlorpyrifos, diazinon, malathion, and fenitrothion, from vegetable samples before their analysis with gas chromatography/mass spectroscopy (GC/MS). Magnetic graphene@Fe3O4@SiO2@TiO2 nanocomposites were synthesized as a unique sorbent using the sol-...
Mon, 11/09/2020 - 00:00
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium, capable of contaminating several foodstuffs. OTA damages primarily the kidneys, and is suspected to be a carcinogenic substance, thus maximum levels for OTA in foodstuffs have been established in the EU. Italian Ministry of Health suggested a maximum level of 1 μg/kg OTA in pork meat and derived products. In this...
Mon, 11/09/2020 - 00:00
Infections involving biofilms are difficult to treat due to increased resistances against antibiotics and the immune system. Hence, there is an urgent demand for novel drugs against biofilm infections. During our search for novel biofilm inhibitors from fungi, we isolated linoleic acid from the ascomycete Hypoxylon fragiforme which showed biofilm inhibition of several bacteria at sub-MIC...
Sun, 11/08/2020 - 22:14
Understanding maternal‐to‐child transmission routes is key to lowering, and ultimately preventing, the exposure of Ebola virus (EBOV) in pediatric populations. We undertook a systematic review of the available scientific literature to determine whether EBOV can be transmitted through breast milk and to describe the outcomes of the infants that ingested EBOV laboratory‐confirmed breast milk....
Sun, 11/08/2020 - 22:13
Between 2012 and 2018 we mapped near‐peak seasonal snow depths across two swaths covering 126 km2 in Northern Alaska using aerial structure‐from‐motion photogrammetry and lidar surveys. The surveys were validated by over a hundred thousand ground‐based depth measurements. Using a quantitative method for identifying drift areas, we conducted a snowdrift census that showed on average 18% of the...
Sun, 11/08/2020 - 22:13
The volume of water stored in seasonal wetlands is a fundamental but difficult to measure variable for developing a physical understanding of wetland behavior. For seasonal wetlands that are a major source of water for rice and fish production, this physical understanding is key to planning for water‐food security and ecosystem services. This study quantified variations in volumetric storage for...
Sun, 11/08/2020 - 22:13
Small ruminant brucellosis remains endemic in many low and middle‐income countries (LMICs), where it poses a major economic and public health burden. Lack of resources to support long‐term vaccination, inherent characteristics of small ruminant production systems such as mixing of different flocks for grazing and limitations of the vaccines currently available, which can induce abortion in...
Sun, 11/08/2020 - 22:13
Salmonellosis is one of the most important bacterial diseases in pigeons. This study aimed to estimate the prevalence of Salmonella spp. in domestic pigeons (Columba livia f. domestica) in Poland, its antimicrobial susceptibility (both phenotypic and genotypic), and its capability for biofilm formation. The presence of selected virulence genes, nucleotide homology of selected genes, and...
Sun, 11/08/2020 - 22:13
Porcine circovirus type 3 (PCV3) is considered a new circovirus and since it first description has been widely reported in most of the swine‐producing countries. Multisystemic inflammation and reproductive failure are consistent and concerning issues associated with PCV3 infection. This report describes the clinical and pathological features of a chronic reproductive disorder in a swine herd in...
Sun, 11/08/2020 - 22:13
Mycobacterium (M.) bovis can infect cats and is a demonstrated zoonosis. We describe an outbreak of M. bovis in pet cats across England and Scotland associated with feeding a commercial raw food diet. Forty‐seven cats presented with (pyo)granulomatous lesions, lymphadenopathy, pulmonary and/or alimentary disease over a one‐year period where M. bovis infection was suspected or definitively...
Sun, 11/08/2020 - 22:13
The purpose of this study was to investigate the dietary effects of using essential oil from sour lemon peel (Citrus limon) on growth performance, carcass composition, blood and serum parameters, and liver enzymes of juvenile rainbow trout (Oncorhynchus mykiss) exposed to deltamethrin (DMN) pesticide. Treatments with no lemon peel essential oil (LPEO) and without DMN (control), as well as four...
Sun, 11/08/2020 - 06:08
Author(s): Cemil Kurekci, Seyda Sahin, Ewelina Iwan, Renata Kwit, Arkadiusz Bomba, Dariusz Wasyl There has been an increase in the number of reports on Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) isolated from animals and humans. Recent studies using whole genome sequencing (WGS) have provided evidence on the likely contribution of a unique conjugative megaplasmid (pESI; ~...

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