Research Publications from Peer-Reviewed Journals

Thu, 01/09/2020 - 03:38
Author(s): Xing-Ping Li, Ruan-Yang Sun, Jia-Qi Song, Liang-Xing Fang, Rong-Min Zhang, Xin-Lei Lian, Xiao-Ping Liao, Ya-Hong Liu, Jun Lin, Jian SunABSTRACTObjectivesTo characterize the colistin-resistant bacterial population in the gut and assess diversity of mcr-1 transmission within a single individual.MethodsLarge numbers of isolates (>100 colonies/chicken cecum sample) were collected from...
Thu, 01/09/2020 - 03:36
Author(s): Lorena Mendes Rodrigues, Luana Aparecida Sales, Paulo Rogério Fontes, Robledo de Almeida Torres Filho, Monalisa Pereira Dutra Andrade, Alcinéia de Lemos Souza Ramos, Eduardo Mendes RamosAbstractCombined effects of gamma irradiation (0, 3, 6 and 9 kGy) and aging (1 and 14 days) on quality attributes of vacuum-packaged beef from Nellore cattle were evaluated. The meat water holding...
Thu, 01/09/2020 - 03:36
Author(s): Romário Junior Ferreira, Thalles Ramon Rosa, Josimar Ribeiro, Rosângela Cristina BarthusAbstractIn this study, square wave anodic stripping voltammetry using two different types of electrodes (carbon nanotube electrode and graphite electrode) was combined with chemometric methods - partial least squares (PLS) and artificial neural networks (ANN) for determining copper, zinc, cadmium...
Thu, 01/09/2020 - 03:36
Author(s): Jinxia Dai, Changling Jiang, Guanwei Gao, Li Zhu, Yunfeng Chai, Hongping Chen, Xin LiuAbstractThere are few studies for risk assessment of cartap and its metabolites, although cartap is easily transformed into metabolites which could induce higher toxicity. This study aimed to investigate the dissipation pattern of cartap and its metabolites during tea planting, manufacturing and...
Thu, 01/09/2020 - 03:36
Author(s): Roghayeh Jalili, Alireza Khataee, Mohammad-Reza Rashidi, Amir RazmjouAbstractInstant detection of antibiotic residues in dairy products has been remained a challenge. Current methods require careful samples storage and handling, skilled personnel, and expensive instrumentations. Herein, we report the preparation of a ratiometric fluorescent sensor that contains different colored Carbon...
Thu, 01/09/2020 - 03:36
Author(s): Yanan Yuan, Hailiang Nie, Junfa Yin, Yehong Han, Yunkai Lv, Hongyuan YanAbstractThe illegal use of β-agonists often endangers animal-derived food safety. In this study, a selective detection method for β-agonists in swine urine was established via the combination of polymeric ionic liquid-molecularly imprinted graphene oxide-miniaturized pipette tip solid-phase extraction and high-...
Thu, 01/09/2020 - 03:36
Author(s): Joon-Goo Lee, Jieun Anh, Gil-Jin Kang, Dongsul Kim, Youngwon KangAbstractTetrabromobisphenol A (TBBPA) and Hexabromocyclododecanes (HBCDs) are commonly used as brominated flame retardants in large volumes, and accumulate in plants and animals in the environment, and people are exposed to them when consuming food. As many countries are monitoring them in food, it is necessary to develop...
Thu, 01/09/2020 - 03:35
Author(s): Hossein Ahangari Cohan, Mahmoud Jamshidian, Mahdi Rohani, Meysam Moravedji, Ehsan MostafaviAbstractBackgroundThe etiologic agent of tularemia, Francisella tularensis, is transmitted to humans via ingestion of contaminated water or food, arthropods bite, respiratory aerosols, or direct contact with infected animals body fluids or tissues. In the current study, due to the importance of...
Thu, 01/09/2020 - 03:35
Author(s): Prince Pal Singh, Satparkash Singh, B.V. Sunil Kumar, R.S. Sethi, Ramneek VermaAbstractMonitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red...
Thu, 01/09/2020 - 03:34
Author(s): Tasmia, Jasmin Shah, Muhammad Rasul JanAbstractIn the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector. The beads were characterized using...
Thu, 01/09/2020 - 03:31
Campylobacter species are known as biofilm‐forming bacteria in food systems. The aim of this study was to evaluate the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter jejuni and Campylobacter coli isolated from chicken meat. The biofilm‐forming C. jejuni and C. coli strains from chicken meat were investigated using minimum inhibitory concentration (MIC) and...
Thu, 01/09/2020 - 03:29
Although highly accurate molecular processes and various messenger RNA (mRNA) quality control and ribosome proofreading mechanisms are used by organisms to transcribe their genes and maintain the fidelity of genetic information, errors are inherent in all biological systems. Low‐level translation errors caused by an imbalance of homologous and nonhomologous amino acids caused by stress conditions...
Thu, 01/09/2020 - 03:27
Over the course of the last few years, the consumption of insects, known as entomophagy, has sparked increasing interest amongst scientists and environmentalists as a potential solution to the inevitable global food security and sustainability issues humans will be facing in the coming years. Despite the fact that insects have been an integral part of over 2 billion people's diet worldwide, the...
Thu, 01/09/2020 - 03:26
Aquatic pathogen Aeromonas hydrophila produces an array of virulence factors, many of which are excreted proteins that causes infectious disease in fish, reptiles, and humans. Aerolysin, a haemolytic toxin, is the most well-known of the A. hydrophila virulence factors and is encoded by aerA. Although used as a virulence gene marker in several studies, recent whole-genome sequencing data suggest...
Thu, 01/09/2020 - 03:26
Microorganisms are indispensable in the food industry, but wild-type strains hardly meet the current industrial demands due to several undesirable traits. Therefore, microbial strain improvement offers a critical solution to enhance the food industry. Traditional techniques for food microbial improvement, such as the use of chemical mutagens and manual isolation/purification, are inefficient,...
Thu, 01/09/2020 - 03:26
SYJ15 is a highly pathogenic Gram-positive Bacillus sp. with top bud spore newly isolated from dying soft shell turtle. 16SrDNA sequencing showed that it is highly homologous to B. cereus, B. thuringiensis and B. anthracis. Biochemical examinations showed that it belongs to B. cereus. To further study the new pathogen, we conducted whole-genome sequencing based on single-molecular sequencing...
Thu, 01/09/2020 - 03:26
The adverse effects of Cd and Pb on human health have been widely reported. While, there were several typical pollution areas in China which has not been investigated comprehensively and deeply. In this study, 475 soil and 472 plant samples from these pollution areas were analyzed for Cd and Pb with ICP-MS. Here, the pollution levels of different areas were analyzed and compared; the spatial...
Thu, 01/09/2020 - 03:25
A case of fungal keratitis due to Coprinellus radians is reported. To our knowledge, fungal keratitis caused by this species was rare. Fungal hyphae were detected in corneal scrapings, and isolates were identified by morphology and by sequencing the internal transcribed spacer region of ribosomal DNA. The patient was treated with systemic and local antifungal therapy for 5 days, and lamellar...
Thu, 01/09/2020 - 03:25
ABSTRACT The zoonotic disease Q fever caused by the intracellular bacterium Coxiella burnetii remains a global health threat due to its high infectivity, environmental stability, the debilitating nature and the long duration of treatment. Designing new and potent drugs that target previously unexplored pathways is essential to shorten treatment time and minimise antibiotic resistance....
Thu, 01/09/2020 - 03:24
Hepatitis E virus (HEV) RNA was detected in 6.3% and HEV IgG in 40% of 5,033 serum samples from market-weight pigs at 25 slaughterhouses in 10 US states. The prevalent HEV genotype was zoonotic genotype 3, group 2. Blood of HEV-viremic pigs from slaughterhouses may contaminate pork supply chains.
Thu, 01/09/2020 - 03:24
In July 2018, recombinant norovirus GII.Pe-GII.4 Sydney was detected in dogs who had diarrhea in a kennel and in children living on the same premises in Thailand. Whole-genome sequencing and phylogenetic analysis of 4 noroviruses from Thailand showed that the canine norovirus was closely related to human norovirus GII.Pe-GII.4 Sydney, suggesting human-to-canine transmission.
Thu, 01/09/2020 - 03:24
The emergence of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli represent a contemporary public health threat. ESBL and AmpC β-lactamase genes translocate between chromosomes and plasmids, facilitating rapid spread throughout the environment. In this study, ESBL/AmpC producing bacteria were isolated from beef cattle farms with seldom antibiotic use. Eleven...
Thu, 01/09/2020 - 03:24
Due to Earth’s changing climate, the ongoing and foreseeable spreading of mycotoxigenic Aspergillus species has increased the possibility of mycotoxin contamination in the feed and food production chain. These harmful mycotoxins have aroused serious health and economic problems since their first appearance. The most potent Aspergillus-derived mycotoxins include aflatoxins, ochratoxins, gliotoxin...
Thu, 01/09/2020 - 03:24
Given that recent advances in metagenomics have highlighted the importance of intestinal microbes for poultry health, there has been a corresponding search for early manipulation strategies of intestinal microbiota in order to advance immune system development and optimize functional properties of growth. In this study, we used the in ovo technique as an experimental model to address how early...
Thu, 01/09/2020 - 03:23
In this study, the prevalence and antimicrobial resistance of enterotoxigenic Escherichia coli (ETEC) and enteropathogenic Escherichia coli (EPEC) were investigated. Altogether 100 stool samples were collected from diarrheal patients attending the Sheikh Hasina Medical College and Hospital, Tangail, Bangladesh, during the period from March 1 to May 30, 2018. In vivo pathogenic potential of ETEC...
Thu, 01/09/2020 - 03:23
The prevalence of human infections with the zoonotic pathogen Campylobacter jejuni is rising worldwide. Therefore, the identification of compounds with potent anti-pathogenic and anti-inflammatory properties for ...
Wed, 01/08/2020 - 03:27
by Markus Beutler, Sara Plesnik, Marina Mihalic, Laura Olbrich, Norbert Heinrich, Samuel Schumacher, Michael Lindner, Ina Koch, Wolfgang Grasse, Christoph Metzger-Boddien, Sabine Hofmann-Thiel, Harald Hoffmann Rapid diagnosis of tuberculosis (TB) and antibiotic resistances are imperative to initiate effective treatment and to stop transmission of the disease. A new generation of more sensitive,...
Wed, 01/08/2020 - 03:27
by Shih-Chao Lin, Brian D. Carey, Victoria Callahan, Ji-Hyun Lee, Nicole Bracci, Anurag Patnaik, Amy K. Smith, Aarthi Narayanan, Benjamin Lepene, Kylene Kehn-Hall Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety...
Wed, 01/08/2020 - 03:27
by Jilong Qin, Matthew Thomas Doyle, Elizabeth Ngoc Hoa Tran, Renato Morona Shigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin through unknown...
Wed, 01/08/2020 - 03:27
ABSTRACT Hydrothermal vents release reduced compounds and small organic carbon compounds into the surrounding seawater, providing essential substrates for microbial growth and bioenergy transformations. Despite the wide distribution of the marine benthic group E archaea (referred to as Hydrothermarchaeota) in the hydrothermal environment, little is known about their genomic repertoires and...
Wed, 01/08/2020 - 03:27
by Soyoun Hwang, Justin J. Greenlee, Eric M. Nicholson Chronic wasting disease is a transmissible spongiform encephalopathy of cervids. This fatal neurodegenerative disease is caused by misfolding of the cellular prion protein (PrPC) to pathogenic conformers (PrPSc), and the pathogenic forms accumulate in the brain and other tissues. Real-time Quaking Induced Conversion (RT-QuIC) can be used for...
Wed, 01/08/2020 - 03:27
by Dirk Thielen, Karl-Ludwig Schuchmann, Paolo Ramoni-Perazzi, Marco Marquez, Wilmer Rojas, Jose Isrrael Quintero, Marinêz Isaac Marques Climate change poses a critical threat to the Pantanal, the largest wetland in the world. Models indicate an increase in the frequency of extreme precipitation events and extended periods of drought. These changes can amplify consequences for Pantanal’s...
Wed, 01/08/2020 - 03:26
Steryl esters (SEs) are important storage compounds in many eukaryotes and are often prominent components of intracellular lipid droplets. Here, we demonstrate that selected Actino- and Proteobacteria growing on sterols are also able to synthesize SEs and to sequester them in cytoplasmic lipid droplets. We found cholesteryl ester (CE) formation in members of the actinobacterial genera Rhodococcus...
Wed, 01/08/2020 - 03:26
Growing evidence suggests that livestock manure used as organic fertilizer in agriculture may lead to the potential propagation of antibiotic resistance genes (ARGs) from "farm to fork." However, little is known about the impacts of manure fertilization on the incidence of ARGs in the plant-associated microbiomes (including rhizosphere, endosphere, and phyllosphere), which hampers our ability to...
Wed, 01/08/2020 - 03:26
More than 10 years ago, we published the paper describing the mothur software package in Applied and Environmental Microbiology. Our goal was to create a comprehensive package that allowed users to analyze amplicon sequence data using the most robust methods available. mothur has helped lead the community through the ongoing sequencing revolution and continues to provide this service to the...
Wed, 01/08/2020 - 03:26
This study aimed to evaluate the prevalence and genetic characteristics of carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage and river water in the Philippines, which has a typical tropical maritime climate. We collected 83 water samples from 7 hospital sewage and 10 river water sites. CPE were identified using CHROMagar mSuperCARBA, and Gram-negative strains were identified...
Wed, 01/08/2020 - 03:26
The organoarsenical feed additive 4-hydroxy-3-nitrobenzenearsonic acid (roxarsone [ROX]) is widely used and released into the environment. We previously showed a two-step pathway of ROX transformation by Enterobacter sp. strain CZ-1 involving the reduction of ROX to 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA) and the acetylation of 3-AHPAA to N-acetyl-4-hydroxy-m-arsanilic acid (N-AHPAA) (K....
Wed, 01/08/2020 - 03:26
Escherichia coli O157:H7 (EcO157) infections have been recurrently associated with produce. The physiological state of EcO157 cells surviving the many stresses encountered on plants is poorly understood. EcO157 populations on plants in the field generally follow a biphasic decay in which small subpopulations survive over longer periods of time. We hypothesized that these subpopulations include...
Wed, 01/08/2020 - 03:26
Author(s): Wen Luo, Lin Tian, Yingde Gan, Enlong Chen, Xuejuan Shen, Junbin Pan, David M. Irwin, Rui-Ai Chen, Yongyi ShenAbstractAvian influenza A viruses (AIVs) classify into 18 hemagglutinin (HA) and 11 neuraminidase (NA) subtypes. Even though H1N1 and H3N2 subtypes usually circulate among humans leading to infection, occasionally, H5, H6, H7, H9, and H10 that circulate in poultry also infect...
Wed, 01/08/2020 - 03:26
Author(s): Tatiana Umpeleva, Kseniya Belousova, Ludmila Golubeva, Tatiana Boteva, Irina Morozova, Anna Vyazovaya, Igor Mokrousov, Natalia Eremeeva, Diana VakhrushevaAbstractThe aim of this study was to perform a molecular characterization of Mycobacterium tuberculosis strains circulating in one of the “closed” Russian cities under conditions of the limited population migration and high HIV...
Wed, 01/08/2020 - 03:25
Author(s): Xifeng Qiao, Pingchao Li, Jianan He, Zeshu Yu, Jiaxing Chen, Liangge He, Xue Yu, Haoran Lin, Danqi Lu, Yong ZhangAbstractScavenger receptors play a central role in defending against infectious diseases in mammals. However, the function of SRECII remains unknown in teleost fish. In this study, type F scavenger receptor expressed by endothelial cells-II (SRECII) cDNA sequence was first...
Wed, 01/08/2020 - 03:24
Author(s): Kang Xie, Hongyun Hu, Sihua Xu, Tongzhou Chen, Yongda Huang, Yuhan Yang, Fu Yang, Hong YaoAbstractThermal treatment could effectively realize the detoxification of heavy metals in municipal solid waste incineration (MSWI) fly ash through the approach of removal or stabilization process. To lower the operating temperature and suppress the evaporation of heavy metals, a molten salts (...
Wed, 01/08/2020 - 03:24
Author(s): Esteban Avigliano, Jonatan Schlotthauer, Barbara Maichak de Carvalho, Mirna Sigrist, Alejandra V. VolpedoAbstractMonomethylarsonic acid (MMA), dimethylarsinic acid (DMA), inorganic arsenic (iAs), and non-toxic species (i.e. arsenobetaine) were determined by HPLC-ICP-MS as mg kg-1 wet weight in muscle, liver and gill of catfish (Genidens barbus) from Argentina (N = 12) and from Brazil (...
Wed, 01/08/2020 - 03:24
Author(s): Zhen Jia, Yanhong Liu, Chang-An Hwang, Lihan HuangAbstractClostridium perfringens is a strictly anaerobic pathogen that requires absence of oxygen for its growth in laboratory experiments, which is usually attained by using an anaerobic chamber or anaerobic jars. However, it has been demonstrated that C. perfringens may survive for short periods of times due to its adaptive response to...
Wed, 01/08/2020 - 03:23
Author(s): João Anes, Scott V. Nguyen, Athmanya K. Eshwar, Evonne McCabe, Guerrino Macori, Daniel Hurley, Angelika Lehner, Séamus FanningAbstractAntimicrobial resistance reported in bacteria of animal origin is considered a major challenge to veterinary public health. In this study, the genotypic and phenotypic characterisation of twelve Escherichia coli isolates of bovine origin is reported....
Wed, 01/08/2020 - 03:23
Author(s): Dong Hu, Renfu Lu, Yibin YingAbstractUnderstanding optical properties of food and agricultural products is essential to apply optical techniques for quality and safety assessment. This research was aimed at optimizing the frequency region through an inverse algorithm for better quantification of the optical absorption (μa) and reduced scattering (μs´) coefficients of two-layered food...
Wed, 01/08/2020 - 03:22
Author(s): Peter Reid, Nicholas C.K. Heng, John D. Hale, Deepti Krishnan, Julian Crane, John R. Tagg, Trudy J. MilneAbstractIn order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations...
Wed, 01/08/2020 - 03:22
Author(s): Luca Michelutti, Michela Bulfoni, Emanuele NencioniAbstractIntroduction: Flow cytometry is a powerful and sensitive technique able to characterize single cells within a heterogeneous population. Different fluorescent dyes can be combined and used together to analyze a great variety of parameters simultaneously. In particular, flow-cytometry allows to measure viability and vitality of...
Wed, 01/08/2020 - 03:22
Author(s): André Alberto Weber, Camila Ferreira Sales, Francisco de Souza Faria, Rafael Magno Costa Melo, Nilo Bazzoli, Elizete RizzoAbstractEnvironmental disasters such as the rupturing of mine tailings dams are a major concern worldwide. In the present study, we assess the effects of the release of mine waste due to the rupture of the Fundão dam on two native fish species (Hoplias intermedius...
Wed, 01/08/2020 - 03:22
Author(s): Xiaoying Lin, Jiating Zhao, Wei Zhang, Lina He, Liming Wang, Dunhu Chang, Liwei Cui, Yuxi Gao, Bai Li, Chunying Chen, Yu-Feng LiAbstractEnvironmental pollutants like methylmercury (MeHg) can bring devastating neurotoxicity to animals and human beings. Gut microbiota has been found to demethylate MeHg and promote the excretion of Hg through feces. However, the impacts of MeHg on gut...

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