Research Publications (Food Safety)

Abstract Aims Within the One Health paradigm, infectious disease surveillance have been developed for domestic and wild animals, leaving the role of captive non‐domestic populations, especially felids in zoos and circuses, less explored. This study addresses the proximity of these captive animals to urban areas, necessitating focused monitoring for potential zoonotic enteropathogens.

To investigate the prevalence of Blastocystis and Dientamoeba fragilis in diarrhea patients and healthy individuals in Corum, Türkiye, fecal samples from 92 diarrhea patients and 50 healthy individuals were collected and evaluated using direct microscopy and molecular methods to screen for bacteria, protozoa, and viruses. The prevalence of Blastocystis was 24.6% in total and more frequent in the healthy group (30.0%).

Vegetables, especially those eaten raw, have been implicated in several foodborne disease outbreaks. Since multiple vegetable matrices and hazards are involved, risk managers have to prioritize those with the greatest impact on public health to design control strategies. In this study, a scientific-based risk ranking of foodborne pathogens transmitted by leafy green vegetables in Argentina was performed.

Water is a vital resource to every living thing on the earth. Once the water is contaminated (physically, chemically, biologically, or radiologically), it brought negative impacts to the living thing. This paper provides a brief review of the characterization of biological pollutants in drinking water and their effects on human health.

Introduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis.

Developing effective therapeutics or preventive interventions for important health threats is greatly enhanced whenever accessible models can enable the assessment of clinically important outcomes. While no non-human model is ever perfect, inexpensive in vivo small animal models in such as mice are often of great help in assessing the relevant efficacy of potential interventions.

Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern.

Background Campylobacter infection is associated with impaired growth of children, even in the absence of symptoms. To examine the underlying mechanisms, we evaluated associations between Campylobacter infection, linear growth, and fecal microbial community features in a prospective birth cohort of 271 children with a high burden of diarrhea and stunting in the Amazonian lowlands of Peru.

To identify the free‐living amoeba (FLA) and amoeba resistant bacteria (ARB) accumulated in zebra mussels and in the water in which they are found, mussels were collected at two locations in the Ebro river basin (North East Spain). Free‐living amoebas and bacteria were isolated from mussel extracts and from natural water.

Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry genes for hydrogenases, the enzymes responsible for H2 oxidation and/or production.

Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz Banaei

Publication date: Available online 23 July 2019

Source: Microbial Risk Analysis

Author(s): Ursula Gonzales-Barron, Anne Thébault, Pauline Kooh, Laurence Watier, Moez Sanaa, Vasco Cadavez

Publication date: November 2018

, Volume 93

Author(s): Radka Dziedzinska, Monika Moravkova, Jakub Hrdy, Iva Slana, Hana Vlkova, Hana Kunstovna, Petra Vasickova

Publication date: Available online 2 May 2018


Author(s): Adriana Calderaro, Monica Martinelli, Mirko Buttrini, Sara Montecchini, Silvia Covan, Sabina Rossi, Francesca Ferraglia, Paolo Montagna, Federica Pinardi, Sandra Larini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto

Abstract

Aims

To assess diarrheal risks from enteropathogenic E. coli, Giardia, and Cryptosporidium from consuming raw spinach, cabbage, carrots, and tomatoes in Kathmandu Valley, Nepal.

Luther A. Bartelt, David T. Bolick, Jordi Mayneris-Perxachs, Glynis L. Kolling, Gregory L. Medlock, Edna I. Zaenker, Jeffery Donowitz, Rose Viguna Thomas-Beckett, Allison Rogala, Ian M. Carroll, Steven M. Singer, Jason Papin, Jonathan R. Swann, Richard L. Guerrant

Mario Santoro, Nicola D’Alessio, Anna Cerrone, Maria Gabriella Lucibelli, Giorgia Borriello, Gaetano Aloise, Clementina Auriemma, Nunzia Riccone, Giorgio Galiero

Publication date: Available online 2 March 2017
Source:Microbial Risk Analysis

Author(s): Mary E. Schoen, Nicholas J. Ashbolt, Michael A. Jahne, Jay Garland

Publication date: Available online 16 January 2017


Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones

Publication date: Available online 9 November 2016
Source:Microbial Risk Analysis

Author(s): Michael A. Jahne, Mary E. Schoen, Jay L. Garland, Nicholas J. Ashbolt

Publication date: 15 November 2016
, Volume 195

Author(s): Mirjam Duijvestijn, Lapo Mughini-Gras, Nancy Schuurman, Wim Schijf, Jaap A. Wagenaar, Herman Egberink

Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in Ecuador between June and August 2014.

Xian-Min Feng, Wen-Yu Zheng, Hong-Mei Zhang, Wen-Yan Shi, Yao Li, Bai-Ji Cui, Hui-Yan Wang

Background

Giardia lamblia is one of the most common infectious protozoans in human that may cause diarrhea in travelers. Searching for antigens that induced effectively protective immunity has become a key point in the development of vaccine against giardiasis.

Methodology/Principal Findings

Summary

Sara M. Pires, Christa L. Fischer-Walker, Claudio F. Lanata, Brecht Devleesschauwer, Aron J. Hall, Martyn D. Kirk, Ana S. R. Duarte, Robert E. Black, Frederick J. Angulo

Background