Research Publications (Food Safety)

This study aimed to determine the prevalence of Staphylococcus aureus subclinical mastitis and to genotype the S. aureus isolates using the 16S-23S rRNA intergenic spacer (RS-PCR) method. In addition, the genes responsible for adherence, biofilm formation, host evasion, tissue necrosis, methicillin resistance, and enterotoxin production of S. aureus were investigated. The overall prevalence of S.

Our study was undertaken to select determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared among hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks.

Genotyping of Staphylococcus aureus isolated from mastitis has become a fundamental tool to understand its complex epidemiology and to evaluate spillover events. The aim of this study was to describe the frequency of genotypes of the S. aureus strains isolated from intramammary infections by spa typing technique, and to evaluate the association between genotypes and the ability to form biofilm under in vitro conditions.

This study aimed to determine the frequency and characterization of Salmonella isolates from food-producing animals and human diarrheal samples in Shiraz, Iran. Overall, 105 Salmonella isolates were obtained from chicken meat (70/100, 70.0%), beef (19/100, 19.0%), lamb (11/100, 11.0%), and human stool (5/295, 1.7%). S. Infantis (40.9%), S. Enteritidis (29.5%), and S.

To determine the antibiotypes and frequency of toxin genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP), 281 nasal swab samples were collected from dogs and dog guardians in Abakaliki, Southeastern Nigeria. Antimicrobial susceptibility testing was determined by disc diffusion technique while detection of toxin genes was carried out by PCR. Exactly 41 (28.7%) and 6 (4.3%) MRSP were obtained from dogs and dog guardians respectively.

This study aimed to identify molecular markers associated with antimicrobial resistance and genotype in isolates of Campylobacter spp. isolated from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C.

Campylobacter (C.) spp. represent one of the most important causes for food-borne bacterial pathogen in humans worldwide. The aim of this study was to investigate metabolic requirements of two Campylobacter strains of different species based on substrate utilisation (in vitro).

On January 2020, an outbreak of acute mortality was detected in the capybara (Hydrochoerus hydrochaeris) population kept in the Cordoba Zoo Conservation Center (CZCC) in Spain. Morbidity and mortality rates were 71%, and the fatality rate was 100%. Macroscopic examination revealed multifocal nodular lesions with fibrinonecrotic exudate in lungs and mesenteric lymph nodes.

Introduction and purpose: Yersinia enterocolitica belongs to the family of Enterobacteriaceae and is a psychrophilic pathogen that is associated with foodborne infections. It usually causes gastroenteritis, mesenteric lymphadenitis, and septicemia. This study aimed to molecular detection, biotyping, and serotyping of Yersinia enterocolitica isolated from chicken livers in Tabriz.

As a part of risk analysis for consumption of meat from wild animals, the prevalence of Campylobacter spp. in wild deer and boar in Japan was investigated. C. hyointestinalis subsp. hyointestinalis (C. hyointestinalis) was isolated from 2.8% (7/253) of the wild deer and 22.1% (71/321) of the wild boar examined.

Bovine mastitis is a major infectious disease affecting dairy animals resulting in enormous economic losses, prolonged antibiotic treatment, reduced milk yield and death of livestock.

A retrospective antimicrobial resistance study of nontyphoidal Salmonella enterica isolates from India during 1990–2017 was conducted to study the microbial susceptibility to antibiotics.

In the current study, we investigated the antibacterial activity of main quorum sensing autoinducers of Pseudomonas aeruginosa, C12-HSL and C4-HSL, against MDR Staphylococcus aureus isolates and their synergistic effects with some common antibiotics. Forty clinical isolates of S. aureus were collected and their antibiotic susceptibility pattern was evaluated. Then, 10 resistant isolates were selected for further studies.

Author(s): Phirabhat Saengsawang, Decha Pangjai, Gunn Kaewmongkol, Tawin Inpankaew

Author(s): Tom Clune, Amy Lockwood, Serina Hancock, Andrew N. Thompson, Sue Beetson, Mieghan Bruce, Angus J.

Author(s): Sophia Inbaraj, Ravi Kant Agrawal, Prasad Thomas, Chandra Mohan, S Agarwal R.K, Med Ram Verma, Pallab Chaudhuri A retrospective antimicrobial resistance study of nontyphoidal Salmonella enterica isolates from India during 1990-2017 was conducted to study the microbial susceptibility to antibiotics.

Author(s): Sara Pahlavanzadeh, Rahem Khoshbakht, Hami Kaboosi, Elham Moazamian The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S.

Author(s): S.C.L. Santos, M.M.S. Saraiva, A.L.B. Moreira Filho, N.M.V. Silva, C.M.G. De Leon, L.A.F. Pascoal, P.E.N. Givisiez, W.A. Gebreyes, C.J.B. Oliveira Abstract Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S.

Author(s): I. Shakuntala, A.A.P. Milton, R.K. Sanjukta, Kaushik Kakoty, Amarjit Karam, Anirban Dutta, K. Puro, Arnab Sen, Samir Das, Sandeep Ghatak Abstract In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA.

Author(s): Amanda Carvalho Rosado Ferreira, Thallyta Maria Vieira, Dircéia Aparecida da Costa Custódio, Maria Norma Melo, Célia Maria Ferreira Gontijo, Andrey Pereira Lage, Elaine Maria Seles Dorneles

Author(s): Shahin fakour, Rojin Jamali, Elham Ahmadi

Author(s): Hend A. Abdelmageed, Ahmed S. Mandour, Attia A. El Gedawy, Mohamed Fawzy, Salim Al-Rejaie, Tetsuya Furuya, Mahmoud Ezzat

Introduction
Dogs are known as asymptomatic carriers forCampylobacter jejuni. The number of pet dogs is increasing in Egypt in the last decade.

Objective
This study aimed to investigate the frequency ofC. jejuni infection in dogs and humans, molecular typing of associated virulence genes, and flaA-SVR gene using sequencing.

Author(s): Mehdi Jaferi, Aliasghar Mozaffari, Maziar Jajarmi, Masoud Imani, Mohammad Khalili

Author(s): Israel Barbosa Guedes, Gisele Oliveira de Souza, Katarine de Souza Rocha, Matheus Burilli Cavalini, Manoel Soares Damasceno Neto, Juliana Fernandes de Paula Castro, Antônio Francisco de Souza Filho, Manoel Pierre Negrão, Adriana Cortez, Carla Cristina Guimarães de Moraes, Marcos Bryan Heinemann

Author(s): Ali Wahdan, Emad Mokhtar Riad, Shymaa Enany