Research Publications (Food Safety)

Microbiology Resource Announcements, Ahead of Print. The complete genome sequence is reported for Vibrio harveyi isolate K2014767, isolated from a captive Caribbean spiny lobster (Panulirus argus) during a species-specific mortality event in a public display aquarium in the United States.
 

Microbiology Resource Announcements, Ahead of Print. Complete genome sequencing of aerotolerant Campylobacter jejuni strain S2-20 revealed the presence of a chromosome of 1,695,449 bp and a plasmid of 49,741 bp that contains predicted antimicrobial resistance and type IV secretion system genes. The chromosome harbored several putative oxidative stress genes with potential roles in aerotolerance.

Microbiology Resource Announcements, Ahead of Print. Extended-spectrum β-lactamase-producing non-O1 Vibrio cholerae was isolated from edible Mastacembelus sp. in Vietnam. The genome sequence was sequenced using DNBSEQ-G400 and MinION Mk1b. A plasmid of approximately 183-kb encoding blaCTX-M-55 and blaTEM-1 was detected.

Microbiology Resource Announcements, Ahead of Print. Nine Peruvian isolates of Vibrio parahaemolyticus were characterized through sequencing, revealing the presence of simple sequence repeat, Pir toxin-like genes, and genes associated with antibiotic resistance, toxic components, and transposable elements. These findings expand our understanding of the genetic diversity, disease resistance, and virulence in cultivated shrimp populations in Peru.

Microbiology Resource Announcements, Ahead of Print. Here, we report the draft genomes of 10 Campylobacter strains isolated from the cecal contents of market-age broiler chickens naturally colonized with Campylobacter. Through a comprehensive analysis of these draft genomes, we have unveiled their core genetic elements and several antimicrobial resistance genes.

Microbiology Resource Announcements, Ahead of Print. Salmonella enterica is a zoonotic pathogen and a leading cause of foodborne gastroenteritis in humans. Here, we report the draft genome sequences of two Salmonella Uzaramo isolates, which were isolated from poultry organs during routine post-mortem examination in South Africa. Currently, whole-genome sequences on Salmonella Uzaramo are scanty.

Microbiology Resource Announcements, Ahead of Print. Herein this study, we sequenced the genome of a multidrug-resistant Salmonella enterica serovar Typhimurium strain MBR-MFRK-23 isolated from the liver tissue of a diseased layer chicken. The 4,964,854-bp draft genome comprises 50 contigs with 50.5× coverage and 52.1% GC content and is typed as S. enterica sequence type 19.

Microbiology Resource Announcements, Ahead of Print. This announcement reports the genome of Vibrio lentus VLO8 recovered from the larval culture of Chilean scallop. The genomes of strain VLO8 have two contigs with a total length of 5,499,980 bp, an average G + C content of 44.22%, a total number of protein-coding genes of 6,439, and 170 RNAs.

Microbiology Resource Announcements, Ahead of Print. Staphylococcus aureus is one of the major pathogens causing bovine mastitis, which results in huge economic losses in the dairy industry worldwide. Here, we report genome sequences of 17 S. aureus strains, with three novel sequencing types (ST8495, ST8500, and ST8501) isolated from the milk of dairy cows with subclinical mastitis.

Microbiology Resource Announcements, Ahead of Print. Genome sequencing of highly virulent Salmonella enterica subsp. enterica serovar Javiana strain FARPER-220 (ST-1674) isolated from broiler chickens in Peru revealed multiple virulence factors, antibiotic resistance genes, and invasion-related subcategories. The results provide insights into the potential importance of this strain in causing infections in various animals.

Microbiology Resource Announcements, Ahead of Print. We announce the complete genome sequence of Vibrio parahaemolyticus strain PH1273. This strain was collected from a Penaeus vannamei pond in the Philippines in 2015. Genome analysis revealed that it lacks the gene pirAB responsible for causing acute hepatopancreatic necrosis disease but encode multiple secretion systems and the associated effectors.

Microbiology Resource Announcements, Ahead of Print. We announce the genome sequence of the Staphylococcus gallinarum MTR_B001 strain isolated from the breast muscle of a chicken in 2022 in Bangladesh. This assembled genome had an estimated length of 2,889,393 bp (with 50× genome coverage), 15 contigs, 36 predicted antibiotic resistance genes, and 27 predicted virulence factor genes.

Microbiology Resource Announcements, Ahead of Print. This manuscript reports the complete genome sequence of a Salmonella enterica subsp. enterica serovar Typhimurium strain (designated “Bnaya”), isolated from a dead dairy calf with severe diarrhea in Israel. The isolate exhibited multi-drug resistance, which is highly unusual in bovine Salmonella spp. in Israel, prompting further investigation.

Microbiology Resource Announcements, Ahead of Print. The bacterium Staphylococcus hyicus causes porcine exudative epidermitis in piglets, which represents both health and welfare concerns. Few genome sequences of this pathogen are published. We provide four additional ones to help future genomic analysis of S. hyicus. These are genomes of strains isolated from Canadian swine.

Microbiology Resource Announcements, Ahead of Print. This report describes the genome sequence of the Staphylococcus gallinarum BAU_KME002 strain isolated in Bangladesh in 2021 from a chicken egg surface. Our assembled genome had 50 contigs, an estimated genome length of 2,866,882 bp (with coverage of 90.0×), 36 predicted antibiotic resistance genes, and 28 predicted virulence factor genes.

Microbiology Resource Announcements, Ahead of Print. Salmonella enterica SE20-C72-2 and Escherichia coli EC20-C72-1 were isolated from the edible fish Anabas testudineus in Vietnam. The chromosomes and plasmids from both strains were sequenced using Oxford Nanopore and Illumina sequencing. Plasmids approximately 250 kbp long, encoding blaCTX-M-55 and mcr-1.1, were detected in both strains.

Microbiology Resource Announcements, Ahead of Print. Raw poultry can harbor microbial pathogens. Campylobacter jejuni BSD5, isolated from a critical control point within a poultry production plant, was sequenced. Genome annotation revealed several virulence genes including antibiotic resistance genes in agreement with the phenotypic results, indicating a potential risk of this strain to public health.

Microbiology Resource Announcements, Ahead of Print. We report the genome sequence of a Salmonella enterica subsp. enterica serovar Bispebjerg strain that was isolated from a turkey flock in 2011. The genome analysis of the strain, a rare and multihost serovar, revealed its pathogenic potential due to antimicrobial resistance and a plethora of Salmonella pathogenicity islands and virulence factors.

Microbiology Resource Announcements, Ahead of Print. While Listeria monocytogenes is a foodborne pathogen of global concern, little is known regarding L. monocytogenes populations in Asia. We report the draft genome sequences of three L. monocytogenes strains from chickens in South Korea, which could facilitate understanding the genetic diversity of the regional L. monocytogenes population.

Microbiology Resource Announcements, Ahead of Print. Spotty liver disease (SLD) is as an important cause of disease in egg-producing flocks. The organisms implicated include Campylobacter hepaticus and Campylobacter bilis. Here, we report the complete genome sequence of C. hepaticus strain RBCL71delta and three additional strains, isolated from a layer flock in Georgia, USA.

Microbiology Resource Announcements, Ahead of Print. Here, we report three complete circular genome sequences of Salmonella enterica SalSpp07, SalSpp08, and SalSpp09, which were isolated from chicken meat and skin during quality control on the production line. The genomes were closed using a hybrid assembly method with short and long sequencing reads.

Microbiology Resource Announcements, Ahead of Print. Vibrio natriegens strain PWH3a, isolated from the Texas Gulf Coast, is used as a model organism in marine microbiology. Here, we report the complete genome sequence of strain PWH3a, which has two circular chromosomes, 4,650 coding sequences, 34 rRNA, 4 noncoding RNA (ncRNA), 131 tRNA genes, and one Mu-like prophage sequence.

Microbiology Resource Announcements, Ahead of Print. We report the circularized complete genome sequences, containing a circular chromosome and two circular plasmids, of strains SalSpp05 (4.9 Mbp) and SalSpp10 (4.8 Mbp), which were isolated from chicken carcass rinse water samples; the sequences were obtained by combining Oxford Nanopore Technologies long-read data and Illumina short-read data. Whole-genome alignments indicated that both strains belong to Salmonella enterica.

Microbiology Resource Announcements, Ahead of Print. Here, we present 20 draft genome sequences of Clostridium botulinum type A isolates originating from foodborne outbreaks in the United States and Ethiopia. Publicly available genomes enhance our understanding of C. botulinum genomics and are an asset in bioterrorism preparedness.

Microbiology Resource Announcements, Ahead of Print. The genome sequences of Escherichia coli O157:H7 strain 380-94, which was isolated from a 1994 dry-cured salami outbreak, and a stronger Congo red-binding variant, RV06, were determined using long-read sequencing technology and de novo assembly. Both strains possessed one chromosome and one plasmid. Strain RV06 possessed a 4,769-bp deletion in the rcs region.