Research Publications (Food Safety)

Foodborne pathogens pose serious risks to human health and food safety. More than one pathogen exists in seafood. It is difficult for conventional culture methods to detect some foodborne pathogens simultaneously. Probe-based loop-mediated isothermal amplification is a feasible tool for achieving multi-objective detection of bacterial pathogens in a short time.

As a typical halophilic bacterium, Vibrio parahaemolyticus (V. parahaemolyticus) is regarded as one of the marine seafood-borne pathogens causing severe illnesses in humans and aquatic animals. Herein, a novel single primer isothermal amplification (SPIA) technique which was modified with SYBR Green II was successfully developed for the visual detection of V. parahaemolyticus.

Vibrio cholerae is an important food-borne pathogenic bacterium that causes human disease, resulting in economic losses worldwide. Rapid diagnosis of cholera infection is important to avoid potential spread of disease. In this study, recombinase-aided amplification (RAA) primers labeled with biotin and fluorescein isothiocyanate (FITC) at 5′ end were prepared for the ctxA pathogenic gene of V. cholerae.

Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common and widely distributed food-borne bacteria. Thus, it is necessary to establish a rapid, specific, and sensitive detection approach for V. parahaemolyticus. The strategy of combining immunomagnetic separation method (IMS) and polymerase chain reaction (PCR) measure was established successfully for V.