Research Publications (Food Safety)

Journal of Separation Science, Accepted Article. Endocrine disrupting chemicals are chemicals that interfere with any aspect of the endocrine system. Several natural and synthetic chemicals including pesticides have been identified as endocrine disruptors, which potentially inhibit the reproductive activity of the hormonal system. The pervasive occurrence with trace level concentrations and extensive variety are the reported characteristics of these chemicals.

Journal of Separation Science, Accepted Article. Bubble-bursting flow microextraction combined with gas chromatography as a green and sustainable microextraction method is used to determine some organophosphorus pesticide residues in water samples. The extraction process occurs at the surface of liquid-gas contact, where the analytes interact with the gas molecules in the bubble. The analytes are transferred to the surface of the sample solution by moving the gas bubbles upwards.

Journal of Separation Science, Accepted Article. Pesticides and veterinary drugs are generally employed to control pests and insects in crop and livestock farming. However, remaining residues are considered potentially hazardous to human health and the environment. Therefore, regular monitoring is required for assessing and legislation of pesticides and veterinary drugs. Various approaches to determining residues in various agricultural and animal food products have been reported.

Journal of Separation Science, Accepted Article.The intensive application of pesticides to increase crop production has resulted in contamination of the agricultural products. Due to their occurrence at trace levels and the complexity of food samples, analysis of pesticide residues requires selective and efficient sample preparation methods.

Journal of Separation Science, Accepted Article. In this study, a lab‐made Parallel‐Single Drop Microextraction methodology using the magnetic ionic liquid trihexyltetradecylphosphonium tetrachloromanganate (II) as extraction solvent was developed to determine the pesticides tebuconazole, pendimethalin, dichlorodiphenyltrichloroethane, and dichlorodiphenyldichloroethylene in human urine samples.

This study assesses the extraction of 11 pharmaceuticals, 5 pesticides, 5 perfluoroalkyl substances and 2 illicit drugs in haemolymph from (Mytilus Galloprovincialis). Four extraction procedures using Phree™ Phospholipid Removal cartridges were tested using different volumes of methanol (400 μl and 600 μl) and acetonitrile (300 μl and 450 μl). The pollutants were determined by HPLC‐MS/MS.

A sample pretreatment method based on the combination of a three‐phase solvent extraction system and deep eutectic solvent‐based dispersive liquid–liquid microextraction has been introduced for the extraction of four organochlorine pesticides in cocoa samples before their determination by gas chromatography–electron capture detection. A mixture of sodium chloride, acetonitrile, and potassium hydroxide solution is added to cocoa bean or powder.

Detecting pesticide residues in human serum is a challenging process. This study developed and validated a method for the extraction and recovery of residues of multiple classes of pesticides from serum using one reagent. Salt‐assisted acetonitrile extraction and HPLC with quadrupole time of flight MS/MS were used to quantitate 34 pesticides classified in nine groups of chemicals in human serum samples, which are frequently detected in food.

A new sol‐gel hybrid methyltrimethoxysilane‐chloropropyltriethoxysilane was prepared as sorbent for solid phase extraction. The extraction efficiency of the prepared sol‐gel hybrid methyltrimethoxysilane‐chloropropyltriethoxysilane were assessed by using three selected organophosphorus pesticides namely chlorpyrifos, profenofos and malathion. Gas chromatography‐mass spectrometry was used for detection of organophosphorus pesticides.

Monitoring biological samples at trace levels of chemicals from anthropogenic actions such as pesticides, pharmaceuticals and hormones has become a very important subject. This work describes a method for the determination of 8 compounds of different chemical classes in human urine samples. Dispersive liquid‐liquid microextraction based on magnetic ionic liquids was used as the sample preparation procedure.

ABSTRACT Pesticides have posed significant threats to aquatic ecosystems, yet little is known about their transformation products. The challenge is to simultaneously analyze various pesticides and transformation products in water as they have distinct physicochemical properties.

This paper describes the synthesis and application of a magnetic amino‐functionalized hollow silica‐titania microsphere as a new sorbent for magnetic dispersive micro‐solid phase extraction of selected pesticides in coffee bean samples. The sorbent was fully characterized by Fourier transforms infrared spectroscopy, field emission scanning electron microscopy, transition electron microscopy, energy‐dispersive X‐ray spectroscopy, and vibrating sample magnetometer techniques.

A new version of magnetic solid phase extraction performed in a narrow bore tube has been proposed for the extraction and preconcentration of different pesticides from various vegetable and fruit juices followed by gas chromatography. A few mg of C8@SiO2@Fe3O4 nanoparticles are added into an aqueous sample solution placed in a narrow bore tube. The sorbent particles move down through the tube under gravity force and are collected at the end of the tube by applying an external magnetic field.

A rapid and effective method was developed for the analysis of 39 pesticide residues in porcine meat with dual‐layer multi‐plug filtration cleanup and gas chromatography‐tandem mass spectrometry detection. The cleanup process was performed with columns packed with two layers, namely multi‐walled carbon nanotubes, C18 and anhydrous magnesium sulfate (MgSO4) as top layer, while mixture of florisil and MgSO4 as bottom layer.

This work provides a simple and rapid method for synthesis uniform simetryn imprinted nanoparticles, which can be used to pretreat the tested samples before detecting. A series of computational approach were employed for design simetryn‐imprinted polymer. Based on the conclusion of theoretical calculation, the simetryn imprinted nanoparticles were synthesized using simetryn as template, methacrylic acid as monomer with different solvent volume and synthesis conditions.

A stir bar sorptive extraction method coupled with deep eutectic solvent based solidification of floating organic droplets–dispersive liquid–liquid microextraction has been used for the simultaneous derivatization and extraction of some acidic pesticides in tomato samples. In this method, initially the analytes are adsorbed on a coated stir bar from tomato juice filled in a narrow tube.

Considering the importance of developing a new analytical approach for pesticide residue detection for the sake of ensuring food safety, a beta‐cyclodextrin based molecularly imprinted polymers were prepared for selective determination of carbendazim. The polymers consist of a porous and hollow structure demonstrating the selective abundant adsorption sites for carbendazim molecule.

The AOAC 2007.1 Quick, Easy, Cheap, Effective, Rugged and Safe official method, together with gas chromatography coupled to a triple quadrupole mass spectrometer was applied for the analysis of 38 multiclass pesticides from dried fruits typically cultivated and exported from Colombia: uchuva (Physalis peruviana), lulo (Solanum quitoense), guanabana (Anona muricata) and pitahaya (Hylocereus undatus).

Beauverolides (beauveriolides) are abundant biologically active cyclodepsipeptides produced by many entomopathogenic fungi, including those that are used as biopesticides. Beauverolides act as cholesterol acyltransferase inhibitors in humans; thus, their mode of action has been the subject of pharmacological and clinical research. The cost‐effective analytical methods are needed for fast, routine laboratory analysis of beauverolides.

Three types of molecularly imprinted solid phase microextraction fibers were fabricated through sol‐gel method using diazinon, parathion‐methyl and isocarbophos as templates, respectively, and assembled together to construct a multifiber for analysis of organophosphorus pesticides in complex matrices. The multifiber provided large extraction capacity and high imprinting factor up to 3.89.

In this study, an effective speed‐regulated directly suspended droplet microextraction method was developed to condense pesticide residues from teas through dispersive solid‐phase extraction prior to analysis by GC–MS/MS. The extractant was intentionally dispersed into the sample solution in the form of globules through high‐speed agitation. This procedure increases the contact area between the binary phases and shortens the distribution equilibrium time.

Molecular imprinted polymers prepared by Pickering emulsion method with lignin as stable particle were used as dispersants in matrix solid phase dispersion, followed by ultra performance liquid chromatography‐tandem mass spectrometry analysis to research the degradation behavior of matrine in soil. The molecular imprinted polymers have regularly morphology and uniformly particles. Moreover, the adsorption experiments and the conditions of matrix solid phase dispersion were discussed.

ABSTRACT In this study, quick, easy, cheap, effective, rugged and safe combined with dispersive liquid‐liquid microextraction is developed for extraction of ten pesticides in complex sample matrices of water and milk. In this regard, effective factors of proposed extraction technique combined with gas chromatography‐flame ionization detector were designed, modeled and optimized using central composite design, multiple linear regression and Nelder‐Mead simplex optimization.

Ion chromatography coupled with mass spectrometry is an established technique for determination of ionic analytes, however, sophisticated buffer removal equipment is required to eliminate inorganic compounds from the eluate before introduction into the ion source of mass spectrometer. A standard high‐performance liquid chromatography coupled with tandem mass spectrometry setup using an ion exchange column (Metrosep® A Supp 5) is proposed as an alternative approach.

The aim of this work was to develop an efficient method for the selective extraction and analysis of fenoxycarb, a carbamate pesticide, in mussel samples using a molecularly imprinted solid‐phase extraction device. The optimization of molecularly imprinted polymer synthesis was performed using the experimental design under the response surface methodology approach.