Research Publications (Food Safety)

The present study is based on the application of H2S as an exogenous antidote in Spinacia oleracea (spinach) plants grown in Cd-contaminated (50 ppm) soil. The different doses of H2S in the form of NaHS (10, 50, 100, 200, and 500 μM) have been applied as a foliar spray to regulate the physiological attributes under Cd toxicity.

A major obstacle to agricultural production and yield quality is heavy metal contamination of the soil and water, which leads to lower productivity and quality of crops. The situation has significantly worsened as a result of the growing population and subsequent rise in food consumption. The growth of nutrient-rich plants is hampered by lead (Pb) toxicity in the soil. Brassica oleracea L.

Cadmium (Cd) toxicity in leaves decreases their photosynthetic efficiency by degrading photosynthetic pigments, reducing the activity of gas exchange parameters and photosystem II (PSII), and producing reactive oxygen species. Although acetone O-(4-chlorophenylsulfonyl) oxime (AO) alleviates stress due to heavy metals in plants, its effects on the photosynthetic apparatus and redox balance under Cd stress are not clear.

Nitric oxide (NO) is known to induce plant resistance for several environmental stresses. The protective roles of NO in cadmium (Cd) toxicity have been well documented for various plant species; nevertheless, little information is available about its molecular regulation in improving Cd tolerance of barley plants.

The main aim of the current study was to investigate the role of Cistus salviifolius leaves extract (CSE) in alleviating the toxic effect of cadmium (Cd) in sorghum (Sorghum bicolor) plants. The plants exposed to Cd (200 µM) exhibited limited growth, reduced biomass, and chlorophyll content compared to unstressed ones. Nevertheless, supplementation of CSE restored the negative effect of Cd and increased biomass and pigment content.

The arsenic (As) contamination demands its remediation from the environment which is naturally possible by the application of Pteris vittata L. However, biomonitoring of phytoremediation potential of P. vittata at chromosomal and DNA level is still meager. The present study was designed to biomonitor the phytoremediation efficiency of P. vittata through phytotoxic and cyto-genotoxic biomarkers assessment using Trigonella foenum-graecum L.

The present study reports the effect of different seasons on polyphenol content and antioxidant potential of ethanolic, methanolic, ethyl acetate, and aqueous extracts of leaves, stems, and roots of Premna integrifolia. Ethyl acetate extract of leaves (EAEPI) collected in the rainy season showed potent antioxidant activity with highest total phenol (74.33 ± 2.26 µg/mg, gallic acid equivalent), and flavonoid (98.83 ± 0.26 µg/mg, rutin equivalent) content.

The present study reports the effect of different seasons on polyphenol content and antioxidant potential of ethanolic, methanolic, ethyl acetate, and aqueous extracts of leaves, stems, and roots of Premna integrifolia. Ethyl acetate extract of leaves (EAEPI) collected in the rainy season showed potent antioxidant activity with highest total phenol (74.33 ± 2.26 µg/mg, gallic acid equivalent), and flavonoid (98.83 ± 0.26 µg/mg, rutin equivalent) content.

To increase food production, prevalent agricultural malpractices such as intensive use of fertilizers and pesticides have led to degradation of the ecosystem. In this situation, there is a need to encourage eco-friendly and sustainable methods for improving crop production under ever increasing abiotic stress conditions. One such method can be through use of arbuscular mycorrhizal fungi (AMF or AM fungi). Soil microorganisms such as AMF serve as a link between plants and the soil resources.

In the present study, the potential role of hydrogen peroxide (H2O2) and nitric oxide (NO) has been well recorded in the induction of cadmium (Cd) stress tolerance in cyanobacteria. In this regard, H2O2 and SNP (sodium nitroprusside, NO donor), were applied to Nostoc muscorum and Anabaena sp. exposed to Cd (6 µM) stress, to analyze different physiological and biochemical parameters.

Salinity is a significant constraint for plant survival and productivity. Therefore, an immediate solution to this problem is sought to meet the human population's food demands. Recently, Menadione sodium bisulphite (MSB) has emerged as a significant regulator of plant defense response under abiotic stress. Studies on MSB are scarce, and a few reports on salinity (Arabidopsis and okra) and cadmium stress (okra) are present in the literature.

Cadmium (Cd) is a toxic metal and an environmental pollutant that significantly reduces plant growth and productivity. Proper management can ameliorate dysfunction and improve the plant growth and productivity exposed to Cd. Therefore, the present study was conducted to explore the protective role of the fungicides tebuconazole (TEB) and trifloxystrobin (TRI) in helping wheat (Triticum aestivum L. cv. Norin 61) seedlings to tolerate Cd.

Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. Nevertheless, the interaction between rice (Oryza sativa L.) plants and the molecular cell dynamics induced by lead-methyl jasmonate (MJ) remains unknown. Here, plants were hydroponically exposed to Pb (150 and 300 µM) alone or in combination with 0.5 and 1 µM MJ.

Cadmium (Cd) in soil–plant system can abridge plant growth by initiating alterations in root zones. Hydroponics and rhizoboxes are useful techniques to monitor plant responses against various natural and/or induced metal stresses. However, soil based studies are considered more appropriate in order to devise efficient food safety and remediation strategies.

Cadmium (Cd) is highly toxic metal for plant metabolic processes even in low concentration due to its longer half-life and non-biodegradable nature. The current study was designed to assess the bioremediation potential of a Cd-tolerant phytobeneficial bacterial strain Bacillus sp. SDA-4, isolated, characterized and identified from Chakera wastewater reservoir, Faisalabad, Pakistan, together with spinach (as a test plant) under different Cd regimes.

Tea (Camellia sinensis (L.) O.Kuntze) is an industry-oriented economical crop in India. Among the sap sucking pests, tea mosquito bug (Helopeltis theivora) is one of the most serious pests causing heavy crop loss in tea plantation. Continuous use of chemical pesticides causes environmental pollution and health hazards besides developing pesticide residues in tea powder.

A pot experiment was conducted to examine the effect of foliar application of various levels of ZnSO4 on wheat (Triticum aestivum L.) under cadmium (Cd) stress. Seeds of two wheat varieties i.e., Ujala-2016 and Anaj-2017 were sown in sand filled plastic pots. Cadmium (CdCl2) stress i.e., 0 and 0.5 mM CdCl2 was applied in full strength Hoagland’s nutrient solution after 4 weeks of seed germination.

Impact of spraying 50 µM salicylic acid (SA), lead nitrate soil treatments [1 and 2 mM Pb (NO3)2] and their combinations on parsley leaves (Petroselinum crispum L.) for 3 weeks was studied to evaluate leaf symptoms, photosynthetic pigments, anthocyanin, ultrastructure, malondialdehyde (MDA), soluble proteins, phenolic compounds, and guaiacol peroxidase activity (GPOX).

The recent global climate change has directly impacted major biotic and abiotic stress factors affecting crop productivity worldwide. Therefore, the need of the hour is to develop sustainable multiple stress tolerant crops through modern biotechnological approaches to cope with climate change. Hybrid proline rich proteins (HyPRPs) are the cell-wall structural proteins, which contain an N-terminal repetitive proline-rich domain and a C-terminal conserved eight-cysteine motif domain.

The aim of this study was to investigate soil lead pollution on biochemical properties and gene expression pattern of antioxidant enzymes in three wheat cultivars (Morvarid, Gonbad and Tirgan) at flag leaf sheath swollen stage. Lead (Pb(NO3)2) was used at four different concentrations (0, 15, 30 and 45 mg/kg of soil). The leaf and roots samples were taken at late-booting stage (Zadoks code, GS: 45).

Cadmium (Cd) is a heavy metal ion leading to morphological and physiological disorders in plants; a specific toxicity target is the membrane lipids. The total lipids were separated by thin-layer chromatography, and the fatty acid composition of the total (TLs), polar lipids (PLs) and triacylglycerol (TAG)—a neutral lipid—was analyzed in maize seedlings in hydroponics and treated by various Cd concentrations (0–200 µM Cd).

Menthol mint (Mentha arvensis L.) cultivation is significantly affected by the heavy metals like cadmium (Cd) which also imposes severe health hazards. Two menthol mint cultivars namely Kosi and Kushal were evaluated under Cd stress conditions. Impact of plant growth regulators (PGRs) like salicylic acid (SA), gibberellic acid (GA3) and triacontanol (Tria) on Cd stress tolerance was assessed.

Transgenic tobacco (N. tabacum cv. Xanthi nc) expressing Capsicum chinense CchGLP gene that encodes an Mn-SOD, constitutively produces hydrogen peroxide that increase endogenous ROS levels. Previous studies using these plants against geminivirus infections as well as drought stress confirmed that CchGLP expression conferred resistance against biotic and abiotic stresses.

The use of pedunculate oak (Quercus robur L.), along with other tree species, for the afforestation of heavy metal contaminated lands is an attractive prospect. Little, however, is known of Q. robur tolerance and its antioxidative system response to heavy metal exposure. The main objective of the study was to determine the cadmium-induced changes in antioxidative system of pedunculate oak in an attempt to identify molecular mechanisms underlying Cd tolerance.