Research Publications (Food Safety)

This study investigated antimicrobial efficacy of trans‐cinnamaldehyde (TC) and chitosan (CH) along with high pressure processing (HPP) against Cronobacter sakazakii in infant formula. All treatments exhibited varying degrees of C. sakazakii reductions up to undetectable levels. Remarkable deformation and damage in C. sakazakii cells were observed by transmission electron microscopy after the appliation of HPP and bioacive cmpounds.

Lateral‐flow immunochromatographic assay (LFIA) was performed on pastry samples incubated for different time‐periods and the degree of coliform contamination evaluated. Pastries contaminated with ~5.0 log10 (cfu/g) coliforms showed LFIA positivity by 3 hr culture, whereas pastries contaminated with 2.0 log10 (cfu/g) coliforms showed LFIA positivity by 9 hr culture.

Cronobacter sakazakii (C. sakazakii) is an opportunistic, neonatal, and food borne pathogen primarily associated with the contamination of powdered infant formula (PIF). The pathogen is reported to overcome the food safety barriers such as increased acidity, heat treatment, and so on. This study evaluates the thermal tolerance of C.

This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine) caused reductions of 1.8, 1.9, and 1.9 log for E. coli, C.

Enzymatic treatment is capable of removing the biofilm matrix so that the subsequent disinfection can be more effective. Abstract Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of biofilms.

The microbial population of kefir made from Korean kefir grain was profiled via next‐generation sequencing technology at the species level. Interestingly, Acetobacter fabarum was the second most predominent bacteria in kefir. We isolated Acetobacter fabarum DH1801 strain and revealed its potent and broad‐spectrum antimicrobial activity against pathogenic bacteria. This could contribute to the anti‐contamination and probiotic attributes of Korean kefir.

In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and minimum inhibitory concentration (MIC) values from 16.25 to 65.00 μg/mL. Among these bacteria, S.

Journal of Food Safety, EarlyView.

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The occurrence of outbreaks of Cronobacter sakazakii causing necrotizing meningitis in China highlights the need for strain characterization of this pathogenic species. In this study, SMM system was utilized to mine for new molecular markers of C. sakazakii. One C. sakazakii-specific CDS (>ref|NC_009778.1|:c1077329-1076055) with a length of 1,275 bp was used to design a primer set. A PCR assay was developed and optimized to detect C. sakazakii.

Cronobacter species are foodborne pathogens associated with neonatal meningitis, sepsis and necrotizing enterocolitis through consumption of contaminated powdered milk. However, there are no specific targets used for differentiating Cronobacter species. The DNA-based assay and polyphasic analysis have facilitated the detection and typing of Cronobacter species in foodborne outbreaks. In this study, polymerase chain reaction (PCR) and dot hybridization for detecting Cronobacter spp.

A novel procedure on vancomycin, α-glucosidase activity, cephalothin and sucrose for isolation of Cronobacter spp. from powdered infant formula (PIF) was developed. Meanwhile, a comparison between the new procedure, Druggan-Forsythe-Iversen (DFI) and modified lauryl sulfate tryptose (mLST) procedure was also carried out. The recovery rate is higher by the new procedure than those by DFI and mLST procedures.