Research Publications (Food Safety)

Due to the lack of electricity and thermostatic instruments in certain settings for convenient detection of Cronobacter species in powdered infant formula (PIF), a novel investigation was conducted to establish an electricity-free visual detection system for rapid detection of Cronobacter species in PIF.

The U.S. Food and Drug Administration (FDA) conducted a sampling assignment in 2014 to ascertain the prevalence of Cronobacter spp. and Salmonella in the processing environment of facilities manufacturing milk powder.Cronobacter was detected in the environment of 38 (69%) of 55 facilities.

Author(s): Tae Jin Cho, Hye Won Kim, Changhoon Yoo, Sung Woo Kim, Min Suk Rhee

Author(s): Hong Qin, Xiuquan Shi, Liang Yu, Ke Li, Jianwu Wang, Jihua Chen, Fei Yang, Haiyan Xu, Huilan Xu

Aim This research aimed to monitor pharmaceutical water system by sampling water from all treatment stages, identify bacterial isolates from each phase and determine the most suitable methods to control them.

Cronobacter sakazakii (C. sakazakii) is an opportunistic, neonatal, and food borne pathogen primarily associated with the contamination of powdered infant formula (PIF). The pathogen is reported to overcome the food safety barriers such as increased acidity, heat treatment, and so on. This study evaluates the thermal tolerance of C.

J. Strysko et al. Invasive Cronobacter infections among infants are associated with severe neurologic disabilities and death. Early Cronobacter reports typically featured hospitalized and preterm infants and recognized contaminated powdered infant formula (PIF) as a transmission vehicle.

Necrotizing enterocolitis (NEC) is a serious inflammatory intestinal disorder with a high mortality rate, which occurs most commonly in newborn infants. Cronobacter sakazakii, a common contaminant in infant formula, is associated with NEC. However, its role in NEC pathogenesis is unknown, and there are still no effective treatments for NEC. Currently, natural bioactive products have been investigated for their beneficial effects in preventing microbial infection.

This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine) caused reductions of 1.8, 1.9, and 1.9 log for E. coli, C.

Author(s): Yue Zhang, Yongkang Xie, Juming Tang, Shaojin Wang, Lu Wang, Guangfei Zhu, Xingyi Li, Yanhong Liu

Salinity stress is a major challenge to agricultural productivity and global food security in light of a dramatic increase of human population and climate change. Plant growth promoting bacteria can be used as an additional solution to traditional crop breeding and genetic engineering. In the present work, the induction of plant salt tolerance by the desert plant endophyte Cronobacter sp. JZ38 was examined on the model plant Arabidopsis thaliana using different inoculation methods.

Background and Aim

In this study, we established a rapid, simple, and sensitive method for visual and point-of-care detection of Salmonella spp., Cronobacter spp., and Staphylococcus aureus in powdered infant formula (PIF) based on multiplex loop-mediated isothermal amplification (mLAMP) combined with lateral flow dipstick (LFD). Three different species-specific target genes, siiA of Salmonella spp., internal transcribed space (ITS) of Cronobacter spp., and nuc of Staph.

Cronobacter spp. can cause systemic infections, such as meningitis, sepsis, and necrotizing enterocolitis, in immunocompromised patients, especially neonates. Although some virulence factors have been reported previously, the pathogenesis of Cronobacter remains unclear. In this study, we compared genome sequences from different Cronobacter species, sequence types, and sources, with the virulence genes in the virulence factor database.

Author(s): Haiyan Zeng, Chengsi Li, Na Ling, Jumei Zhang, Moutong Chen, Tao Lei, Shi Wu, Xiaojuan Yang, Dandan Luo, Yu Ding, Juan Wang, Shuhong Zhang, Qingping Wu

Cronobacter spp. are important opportunistic foodborne pathogens in powdered infant formula that cause many serious diseases in neonates and infants. In this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle amplification (RCA) for the detection of Cronobacter spp. in milk.

Cronobacter sakazakii is a food-borne pathogen carried in milk powder that can cause severe bacteremia, enterocolitis, and meningitis in newborns, which can lead to death of newborns. Preventing infection by this pathogen is significant to the health of newborns. Since infants and young children are the main target group of C. sakazakii, it is considered that maternal immunity can enhance the protection of newborns. Previous studies showed that two proteins of C.

Author(s): Yi Li, Leyi Zhang, Yuqin Hu, Chengji Hong, Airong Xie, Yuejin Wu, Zhihui, Shangguan, Lingling Mei, Biao Zhou, Yanjun Zhang, Lei Fang

Author(s): Yifang Cao, Ailian Zhou, Donggen Zhou, Xinglong Xiao, Yigang Yu, Xiaofeng Li

Author(s): Song Zhang, Jian Xiong, Wenyong Lou, Zhengxiang Ning, Denghui Zhang, Jiguo Yang

Author(s): Ailian Zhou, Yifang Cao, Donggen Zhou, Shuangfang Hu, Wanjing Tan, Xinglong Xiao, Yigang Yu, Xiaofeng Li

Author(s): Saidou Harouna, Indira Franco, Juan J. Carramiñana, Arturo Blázquez, Inés Abad, María D. Pérez, Miguel Calvo, Lourdes Sánchez

Background

Enzymatic treatment is capable of removing the biofilm matrix so that the subsequent disinfection can be more effective. Abstract Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of biofilms.

Author(s): Paula Vasconcelos Costa, Luiza Vasconcellos, Ingrid Camelo da Silva, Valéria de Mello Medeiros, Stephen James Forsythe, Marcelo Luiz Lima Brandão