Research Publications (Food Safety)

Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants. Strain Cr268 was isolated from imported powdered infant formula in 2009 during routine microbial examination according to ISO-22964 ("Microbiology of the food chain—horizontal method for the detection of Cronobacter spp."). Isolate Cr268 was confirmed to be C.

The microbial population of kefir made from Korean kefir grain was profiled via next‐generation sequencing technology at the species level. Interestingly, Acetobacter fabarum was the second most predominent bacteria in kefir. We isolated Acetobacter fabarum DH1801 strain and revealed its potent and broad‐spectrum antimicrobial activity against pathogenic bacteria. This could contribute to the anti‐contamination and probiotic attributes of Korean kefir.

Blue light (BL) exerts an antimicrobial effect on pathogenic bacteria. It has been hypothesized that its bactericidal activity depends upon the generation of reactive oxygen species (such as anion superoxides) and the resultant cellular damage. However, some aspects of this hypothesis needed to be tested and investigated. Thus, the work conducted herein examined the molecular impact of BL treatment on Cronobacter sakazakii, an emerging foodborne pathogen.

Author(s): Ömer Akineden, Tobias Wittwer, Katrin Geister, Madeleine Plötz, Ewald Usleber

Author(s): Yifei Chen, Qiwu Wen, Shan Chen, Du Guo, Yunfeng Xu, Sen Liang, Xiaodong Xia, Baowei Yang, Chao Shi

Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20L returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable Number.

Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled.

Author(s): Zhibin Liu, Zhiyao Wang, Jinyuan Sun, Li Ni

Author(s): Du Guo, Shuo Wang, Jiahui Li, Fangting Bai, Yanpeng Yang, Yunfeng Xu, Sen Liang, Xiaodong Xia, Xin Wang, Chao Shi

In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and minimum inhibitory concentration (MIC) values from 16.25 to 65.00 μg/mL. Among these bacteria, S.

Author(s): Yawen Lin, Jeyamkondan Subbiah, Long Chen, Tushar Verma, Yanhong Liu

The traditional gold nanoparticle (AuNP) growth-based plasmonic ELISA (pELISA) strictly and directly controlled by reducing reagents can achieve high sensitivity, but it remains fragile toward the surrounding environment. This work developed a sandwich pELISA for Cronobacter detection in powdered infant formula samples by mediating AuNP growth through DNA.

The purpose of the study was to determine if pomegranate (Punica granatum L.) peel contains polyphenolic compounds with antibacterial activity against Cronobacter sakazakii. Minimum inhibitory and bactericidal concentrations of a methanolic extract were found to increase significantly (P < .05) with decreasing temperature (37, 21, 10 °C) and pH (6.0, 5.0, 4.0).

Cronobacter strains harboring CRISPR-Cas systems are important foodborne pathogens that cause serious neonatal infections. CRISPR typing is a new molecular subtyping method to track the sources of pathogenic bacterial outbreaks and shows a promise in typing Cronobacter, however, this molecular typing procedure using routine PCR method has not been established. Therefore, the purpose of this study was to establish such methodology, 257 isolates of Cronobacter sakazakii, C. malonaticus, and C.

ABSTRACT

Publication date: Available online 15 July 2019

Source: LWT

Author(s): Francesca Rita Dinardo, Fabio Minervini, Maria De Angelis, Marco Gobbetti, Michael G. Gänzle

Available online 13 July 2019

Author(s): Xiuqin Lou, Tao Liu, Wei Zhang, Hua Yu, Haoqiu Wang, Shujuan Song, Qi Chen, Zhiguo Fang

Abstract

Cronobacter sakazakii is an emerging foodborne pathogen, which is linked to life-threatening infections causing septicemia, meningitis and necrotizing enterocolitis. These infections have been epidemiologically connected to ingestion of contaminated reconstituted powder infant formula. Even at low water activity C.

Available online 29 June 2019

Author(s): D. Lindsay, S. Laing, K.I. Fouhy, L. Souhoka, A.K. Beaven, T.K. Soboleva, P.K. Malakar

Available online 27 May 2019

Author(s): Dongjie Chen, Yanling Cheng, Peng Peng, Juer Liu, Yunpu Wang, Yiwei Ma, Erik Anderson, Chi Chen, Paul Chen, Roger Ruan

Journal of Food Protection, Volume 82, Issue 6, Page 1082-1088, June 2019.

Mahewu is a fermented cereal beverage produced in Zimbabwe. This study determined the composition and origin of mahewu microbiota. The microbiota of mahewu samples consisted of 3 to 7 dominant strains of lactobacilli and two strains of yeasts. Enterobacteriaceae were not detected. Candida glabrata was present in high cell counts from samples collected in summer but not from samples collected in winter.

Cronobacter turicensis is a food-borne pathogen found in dairy products. It has been reported to cause bacteremia and enteritis in immunocompromised people, especially infants. Cronobacter turicensis has been isolated from various food sources, and contaminated powdered infant formula was found to be the most common source of infection among infants. Although some gene targets are used for the identification of C. turicensis, they are not specific at the species level.

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus