Research Publications (Food Safety)

Publication date: 30 August 2019

, Volume 290

Author(s): Dongjie Chen, Peng Peng, Nan Zhou, Yanling Cheng, Min Min, Yiwei Ma, Qingqing Mao, Paul Chen, Chi Chen, Roger Ruan

Publication date: Available online 28 February 2019

Source: LWT

Author(s): Yunzhe Zhang, Qian Yang, Cong Li, Yaowu Yuan, Wei Zhang

Chengsi Li, Haiyan Zeng, Jumei Zhang, Wenjing He, Na Ling, Moutong Chen, Shi Wu, Tao Lei, Haoming Wu, Yingwang Ye, Yu Ding, Juan Wang, Xianhu Wei, Youxiong Zhang, Qingping Wu Cronobacter spp. are foodborne pathogens that can infect and cause life-threatening diseases in all age groups, particularly in infants and immunocompromised elderly. This study aimed to investigate the prevalence, antibiotic susceptibility, and molecular characteristics of Cronobacter spp.

Escherichia coli O157:H7, Cronobacter spp., and Salmonella spp. are common food-borne pathogens in milk that may cause serious diseases. In the present study, we established a simple, rapid, and specific method to simultaneously detect viable E. coli O157:H7, Cronobacter spp., and Salmonella spp. in milk. Three specific genes, fliC from E. coli O157:H7, cgcA from Cronobacter spp., and invA from Salmonella spp., were selected and used to design primers and probes.

Yichen Bai, Haibo Yu, Du Guo, Shengyi Fei, Chao Shi The aim of this study was to evaluate the survival of Cronobacter sakazakii exposed to vacuum or air packaging, then stored at 4, 10, or 25°C, and the environmental stress resistance of vacuum-packaged or air-packaged bacterial cells were determined by subjecting the cells to reconstituted infant formula at 50°C, in acid (simulated gastric fluid, pH = 3.5), and in bile salt [bile salt solution, 5% (wt/vol)]. A cocktail culture of C.

Available online 6 February 2019

Author(s): Xinjie Song, Shruti Shukla, Myunghee Kim

Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria.

Available online 22 January 2019

Author(s): Qi Wang, Stephen James Forsythe, Xiao-Juan Zhao, Zi-Wei Wang, Dan Li, Dan Ma, Jia-Yue Cao, Jing Zeng

Cronobacter sakazakii is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food formula, often resulting in high mortality rates. Here, we used transcriptome sequencing (RNA-seq) to provide transcriptional insights into the survival of C. sakazakii in desiccated conditions. Our RNA-seq data show that about 22% of the total C. sakazakii genes were significantly upregulated and 9% were downregulated during desiccation survival.

Cronobacter species are a group of opportunistic food-borne pathogens that cause rare but severe infections in neonates. Tolerance to environmental stress in Cronobacter is known; however, factors involved in oxidative stress are undefined. In this study, Cronobacter sakazakii survival, cellular morphology, and biofilm formation in response to oxidative stress were evaluated between the wild type (WT) and an outer membrane protein W (OmpW) mutant.

Abstract

Journal of Food Protection, Volume 82, Issue 1, Page 65-77, January 2019.

Journal of Food Safety, EarlyView.

Xin-jun Du, Xiao-yi Wang, Xuan Dong, Ping Li, Shuo Wang

Publication date: March 2019

Source: LWT, Volume 101

Author(s): Jae-Hyun Yoon, Areum Han, Jeongyeup Paek, Sun-Young Lee

Wei Yong, Baofu Guo, Xiaochao Shi, Tingting Cheng, Mingming Chen, Xiao Jiang, Yanhua Ye, Junning Wang, Guoxiang Xie, Jie Ding

Min Wang, Hengchun Cao, Qian Wang, Tingting Xu, Xi Guo, Bin Liu

Daqu is a spontaneous solid-state cereal fermentation used as saccharification and starter culture in Chinese vinegar and liquor production. The evolution of microbiota in this spontaneous fermentation is controlled by the temperature profile, which reaches temperatures from 50 to 65°C for several days. Despite these high temperatures, mesophilic Enterobacteriaceae (including Cronobacter) and bacilli are present throughout Daqu fermentation.

Julio Parra-Flores, Juan Aguirre, Vijay Juneja, Emily E. Jackson, Ariadnna Cruz-Córdova, Jesus Silva-Sanchez, Stephen Forsythe

We report a case of meningitis in a neonate in China, which was caused by a novel multidrug-resistant Cronobacter sakazakii strain, sequence type 256, capsular profile K1:CA1. We identified genetic factors associated with bacterial pathogenicity and antimicrobial drug resistance in the genome and plasmids. Enhanced surveillance of this organism is warranted.

The first documented isolation of what would become known as Cronobacter sakazakii was from a can of dried milk in 1950, although these organisms have likely existed for millions of years. In 1980, John J. Farmer III, proposed the name Enterobacter sakazakii for what had been known as “yellow-pigmented E. cloacae,” in honor of Japanese bacteriologist Riichi Sakazaki. Over the next decades, E.

Available online 20 September 2018

Author(s): Xiaoyan Pei, Ying Li, Huaning Zhang, Li Zhan, Xiaojie Yu, Guang Lan, Huayun Jia, Ning Li, Dajin Yang, Lingling Mei

In Bogotá, Colombia, a large number of babies are fed with breast milk substitutes made from corn and plantain starch. We found 34.3% of tested samples to be contaminated with Cronobacter spp.; C. sakazakii was the most recovered species. Our findings underscore the risk for contamination of breast milk substitutes.

We describe a case of infection with Cronobacter sakazakii sequence type 494 causing bacteremia and meningitis in a hospitalized late premature infant in Brazil. We conducted microbiological analyses on samples of powdered infant formula from the same batch as formula ingested by the infant but could not identify the source of contamination.

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