Research Publications (Food Safety)

A lattice was designed and fabricated using three-dimensional (3D) printing that allows for the facile transfer of biofilms formed from either Staphylococcus aureus, Staphylococcus epidermidis, or Pseudomonas aeruginosa into a fresh cell culture flask. To enhance biofilm production onto the filaments, three protein-based treatments were compared: fetal bovine serum (FBS), bovine serum albumin (BSA), and fibrinogen (Fb).

Nonspecific binding of proteins from complex food matrices is a significant challenge associated with a biosensor using gold nanoparticles (AuNPs). To overcome this, we developed an efficient EDTA chelating treatment to denature milk proteins and prevent their adsorption on AuNPs. The use of EDTA to solubilize proteins enabled a sensitive label-free apta-sensor platform for colorimetric detection of Staphylococcus aureus in milk and infant formula.

Coagulase-negative Staphylococcus hominis causes bloodstream infections and often can form biofilms on medical devices. This study aimed to improve the current methodology for antimicrobial susceptibility testing (AST) in biofilm-growing S. hominis isolates. Biofilm production of S.

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories. Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition.

Many factors affecting satellitism tests are unclear, and it is difficult to avoid misidentification, even if the medium is properly selected. We investigated the factors causing false-positive results for Haemophilus influenzae and false-negative results for Haemophilus parainfluenzae in the satellitism tests using Staphylococcus aureus as the source of nicotinamide adenine dinucleotide (NAD). H.

Author(s): Sophie Reissier, Isabelle Lazareth, Louise Adjiman, Carine Couzigou, Barbara Vidal, Assaf Mizrahi, Jean Claude Nguyen Van, Benoît Pilmis, Alban Le Monnier, Julie Lourtet-Hascoët

Abstract

Objectives

Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections.

Author(s): Kotková Hana, Cabrnochová Marie, Lichá Irena, Tkadlec Jan, Fila Libor, Bartošová Jana, Melter Oto

Abstract

Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance.

Author(s): Zafer Ata, Esra Buyukcangaz

Available online 9 March 2019

Author(s): Reinhard Sting, Tobias Eisenberg, Maja Hrubenja

Available online 15 February 2019

Author(s): Bo Zhang, Yibeibaihan Maimaiti, Chunyan Liu, Jing Li, Haiye Wang, Haojie Lin, Zhaohui Deng, Xinhong Lu, Xin Zhang

Publication date: Available online 10 December 2016


Author(s): Tiantian Wang, Sanghyo Kim, Jeong Ho An

Publication date: Available online 6 February 2016


Author(s): B. Kramer, J. Thielmann

Publication date: Available online 10 November 2015


Author(s): Gregoire Seyrig, Robert D. Stedtfeld, Dieter M. Tourlousse, Farhan Ahmad, Keara Towery, Alison M. Cupples, James M. Tiedje, Syed A. Hashsham

Publication date: January 2015
, Volume 108
Author(s): Christian Strauss , Andrea Endimiani , Vincent Perreten