Research Publications (Food Safety)

Letters in Applied Microbiology, Accepted Article. The present study evaluated the antibacterial and antibiofilm activity of MHIR in combination with amoxicillin and clindamycin against biofilm-forming MRSA isolated from milk samples of mastitic cows. Microdilution susceptibility testing and microtiter plate assays were used to evaluate the in-vitro efficacy of MHIR and antibiotic combinations against MRSA (n=12).

Letters in Applied Microbiology, Accepted Article. Staphylococcus aureus is one of the main etiological agents causing foodborne diseases. Some strains produce enterotoxins responsible for food poisoning. In addition, they can form biofilms on several surfaces such as plastics, glass, stainless steel making it difficult to eliminate them.

Letters in Applied Microbiology, Accepted Article. CRISPR-Cas system contributes adaptive immunity to protect the bacterial and archaeal genome against invading mobile genetic elements. In this study, an attempt was made to characterize the CRISPR-Cas system in S. coagulans, the second most prevalent coagulase positive staphylococci causing skin infections in dogs.

Letters in Applied Microbiology, Accepted Article. Antimicrobial resistance has silently turned into one of the biggest threats to global health, marking the fall of the Golden age of antibiotics. In the search for antibiotic replacement or enhancement, plant-derived natural compounds have attracted lots of interest. Even though firmly believed, the low resistance tendency of pathogenic bacteria against plant extracts has been scarcely demonstrated.

Letters in Applied Microbiology, Accepted Article. In this work, the antibacterial activity and mechanism of chloroform fraction obtained from aqueous extract of mugwort leaves against Staphylococcus aureus (S. aureus) were investigated. The extract showed obvious antibacterial activity against S.

Letters in Applied Microbiology, Accepted Article. Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T.

Letters in Applied Microbiology, Accepted Article. Artocarpin-rich extract (ARE) was prepared using a green technology and standardized to contain 49.6% w/w artocarpin, while lawsone methyl ether was prepared using a green semi-synthesis. ARE, LME and ampicillin exhibited weak anti-MRSA activity with the MICs of 31.2-62.5 µg/mL.

Letters in Applied Microbiology, Accepted Article. Staphylococcus aureus is a biofilm-producing organism that is frequently isolated from various environments worldwide. Because of the natural resistance of S. aureus biofilm to antibiotics, bacteriophages are considered as a promising alternative for its removal. The bacteriophage vB_SauS_JS02 was isolated from livestock wastewater and showed activity against multidrug-resistant (MDR) S.

Letters in Applied Microbiology, EarlyView.

Letters in Applied Microbiology, Volume 74, Issue 1, Page 73-77, January 2022.

Letters in Applied Microbiology, Accepted Article. High pressure homogenization (HPH) offers new opportunities for food pasteurization/sterilization. Escherichia coli and Staphylococcus aureus suspended in PBS buffer, milk and apple juice at initial concentration of ~106 log10CFU/mL were subjected to HPH treatments up to 200 MPa with inlet temperatures at 4 ‐ 40 ºC. After HPH at 200 MPa with the inlet temperature at 40 ºC, the count of E.

Exudative epidermatitis (EE) or greasy pig disease (GPD) is a contagious disease of pig and endemic worldwide caused by toxigenic strains under genus Staphylococcus. The present study reported an outbreak of GPD in Champhai district of Mizoram adjoining to the southern border of Myanmar. A total of 60 samples were collected from 22 clinically affected animals and processed for isolation and identification of Staphylococcus spp.

Significance and Impact of the Study: Nowadays chemically synthesized drugs are expensive and create damage to nontarget environment. It creates the demand for low toxic drugs to control and need to achieve immediate results. Microbially derived biosurfactant has been prepared and tried as a drug to various biofilm producers, Anopheles vector and cancer cells. These results confirms the potential ability of rhamnolipid in various toxic environment.

Carvacrol has been recognized as an efficient growth inhibitor of food pathogens. However, carvacrol oil is poorly water‐soluble and can be oxidized, decomposed or evaporated when exposed to the air, light, or heat. To overcome these limitations, a carvacrol nanoemulsion was developed and its antimicrobial activity against food pathogens evaluated in this study.

Contamination of ready‐to‐eat (RTE) foods by pathogenic bacteria may predispose consumers to foodborne diseases. This study investigated the presence of bacterial contaminants and their antibiotic susceptibility patterns in three locally processed RTE foods (eko, fufu and zobo) vended in urban markets in Ogun state, Nigeria.

A triplex‐PCR assay was developed and evaluated for rapid detection of methicillin‐resistant Staphylococcus aureus (MRSA) recovered from various biological samples of pig. Three sets of primers were designed to target mecA, 16S rRNA and nuc genes of MRSA. The specific amplification generated 3 bands on agarose gel, with sizes 280 bp for mecA, 654 bp for 16S rRNA and 481 bp for nuc, respectively.

Staphylococci from Sheedal of Northeast India was isolated, identified and characterized. All the isolated Staphylococci were found to be coagulase‐negative. Based on the rpoB gene sequences followed by analysis using NCBI‐BLAST software, seven species of Staphylococcus namely, S. piscifermentans, S. condimenti, S. arlettae, S. sciuri, S. warneri, S. nepalensis and S. hominis were recognized. Phylogenetic analyses revealed three major cluster groups.

The aims of the present study were to examine the occurrence of Staphylococcus spp. in the tonsils of slaughtered pigs in a regional slaughterhouse in Greece, the antibiotic resistance of the Staphylococcus spp. isolates, and the enteroxigenicity of the S. aureus isolates. Staphylococcus spp. were isolated in 70 (48.61%) out of the total 144 tonsil samples. The predominant species was S.

Highly concentrated sugar solutions are known to be effective anti‐microbial agents. However, it is unknown whether this effect is solely the result of the collective osmotic effect imparted by a mixture of sugars or whether the type of carbohydrate used also has an individual chemical effect on bacterial responses, i.e., inhibition/growth.

Food poisoning risk related to the consumption of contaminated food with known foodborne pathogens or antibiotic‐resistant bacteria is currently a serious threat for public health. Thus, pathogenic Methicillin‐resistant Staphylococcus strains are considered as one of the major cause of food‐borne diseases in hospitals.

The aim of the present study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. Seventeen samples of paired follicular fluids and uterine contents collected from post‐slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Further, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also investigated using PFGE analysis.

Attiéké is the major fermented plant food in Côte d'Ivoire. The aim of this study was to identify hazards and critical control points (CCP) in order to implement a HACCP system for the production of attiéké. Physico‐chemical and microbiological analyses were carried out. pH of the cossettes used as raw material for attieke process was slightly acidic (6·5 ± 0·23). But attiéké produced had an acid pH (4·55 ± 0·67).

Significance and Impact of the Study: This paper reports on a simple, robust and scientifically sound method to reduce attachment and biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis to abiotic surfaces using a carefully designed nanoscale topography. This approach can help to reduce the incidence of staphylococcal biofilms and infections without imposing selective stresses on bacteria, thus preventing the creation of resistant strains.

Abstract

Abstract