Research Publications (Food Safety)

Microbiology Resource Announcements, Ahead of Print. Staphylococcus aureus is one of the major pathogens causing bovine mastitis, which results in huge economic losses in the dairy industry worldwide. Here, we report genome sequences of 17 S. aureus strains, with three novel sequencing types (ST8495, ST8500, and ST8501) isolated from the milk of dairy cows with subclinical mastitis.

Microbiology Resource Announcements, Ahead of Print. We announce the genome sequence of the Staphylococcus gallinarum MTR_B001 strain isolated from the breast muscle of a chicken in 2022 in Bangladesh. This assembled genome had an estimated length of 2,889,393 bp (with 50× genome coverage), 15 contigs, 36 predicted antibiotic resistance genes, and 27 predicted virulence factor genes.

Microbiology Resource Announcements, Ahead of Print. The bacterium Staphylococcus hyicus causes porcine exudative epidermitis in piglets, which represents both health and welfare concerns. Few genome sequences of this pathogen are published. We provide four additional ones to help future genomic analysis of S. hyicus. These are genomes of strains isolated from Canadian swine.

Microbiology Resource Announcements, Ahead of Print. This report describes the genome sequence of the Staphylococcus gallinarum BAU_KME002 strain isolated in Bangladesh in 2021 from a chicken egg surface. Our assembled genome had 50 contigs, an estimated genome length of 2,866,882 bp (with coverage of 90.0×), 36 predicted antibiotic resistance genes, and 28 predicted virulence factor genes.

Microbiology Resource Announcements, Ahead of Print. We identified a double-stranded DNA (dsDNA) bacteriophage appearing to belong to Herelleviridae, genus Kayvirus. The bacteriophage, Biyabeda-mokiny 1, was isolated from breast milk using a clinical isolate of Staphylococcus aureus. The genome is 141,091 bp in length and encodes 230 putative coding sequences.

Microbiology Resource Announcements, Ahead of Print. Two lytic double-stranded DNA (dsDNA) bacteriophages, belonging to the family Herelleviridae, were isolated from wastewater in Western Australia. Biyabeda-mokiny 2 appears to belong to the genus Kayvirus, and Koomba-kaat 1 to Silviavirus.

Microbiology Resource Announcements, Ahead of Print. This work describes the draft genome sequence of Streptococcus agalactiae strain AH2, which was isolated from moribund farmed hybrid tilapia (Oreochromis niloticus × Oreochromis aureus) in Saudi Arabia. The draft genome is composed of a single chromosome of 2,104,071 bp, with a GC content of 35.7%.

Microbiology Resource Announcements, Ahead of Print. Staphylococcus aureus is one of the most important bacterial pathogens causing bovine mastitis, which leads to huge economic losses worldwide. Here, we report draft genome sequences and antimicrobial resistance gene profiles of five Staphylococcus aureus strains that were isolated from bovine milk in Pakistan.

Microbiology Resource Announcements, Ahead of Print. Mammaliicoccus sciuri (previously Staphylococcus sciuri) is a frequent colonizer of mammals. We report the draft genomes of a methicillin-resistant strain (2254A) isolated from an armadillo and a methicillin-susceptible strain (6942A) from a cow. Genomes were sequenced using long-read Nanopore sequencing.

Microbiology Resource Announcements, Ahead of Print. We report the isolation, identification, and assemblies of three antibiotic-producing soil bacteria (Staphylococcus pasteuri, Peribacillus butanolivorans, and Micrococcus yunnanensis) that inhibit the growth of Neisseria commensals in coculture.

Microbiology Resource Announcements, Ahead of Print. We report the draft genome sequence of the laboratory strain Staphylococcus aureus NCTC 6571-UB, a strain that was derived from S. aureus NCTC 6571. This strain was selected for sequencing in order to provide information on the genome dynamics and the acquired resistance genes for penicillin G, trimethoprim, and sulfamethoxazole resistance.

Microbiology Resource Announcements, Ahead of Print. Here, we present the complete genome sequence of Staphylococcus edaphicus strain CCM 8731, which was originally isolated from Ross Island, Antarctica. The 2,749,487-bp sequence contains 2,709 predicted genes, with a G+C content of 33.4%. The complete genome was assembled using a hybrid approach with Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing.

Microbiology Resource Announcements, Ahead of Print. Staphylococcus arlettae is commonly found on the skin of animals. Here, we describe the complete genome sequence of S. arlettae AHKW2e (2,649,260 bp; GC content, 33.6%), isolated from a dog’s paws in Hong Kong, established through hybrid assembly and representing the second complete genome sequence of this species.

Microbiology Resource Announcements, Ahead of Print. Here, we describe the whole-genome sequence of Streptococcus iniae strain AH1, which was isolated from moribund farmed hybrid tilapia (Oreochromis niloticus × Oreochromis aureus) in Saudi Arabia. The genome is composed of a single linear chromosome of 2,068,661 bp, with a G+C content of 36.8%.

Microbiology Resource Announcements, Ahead of Print. The USA300 and USA600 clonal lineages are the cause of many serious Staphylococcus aureus infections. Here, we report the complete genomes of two methicillin-sensitive S. aureus strains isolated from the healthy skin of adults in Colorado, which are most phylogenetically similar to the USA300 and USA600 lineages.

Staphylococcus aureus bacteria are ranked among the top five foodborne pathogens in the United States. Here, we report the draft genome sequences of 62 S. aureus isolates that originated from the manufacturing environment of an Illinois bakery and were associated with outbreaks between 2010 and 2011 in the United States.

Staphylococcus aureus can cause mastitis in dairy cattle. We report the genome sequence of a Staphylococcus aureus strain isolated from a dairy cow with a chronic case of mastitis. The infection with this strain of Staphylococcus aureus was not cleared from the animal with antibiotic treatment.

Staphylococcus aureus is one of the most common etiological agents responsible for contagious bovine mastitis. Here, we report the draft whole-genome sequences, with annotations, of 27 S. aureus strains and 3 Staphylococcus species strains that were isolated from Holstein cows with intramammary infection in Canada.

Here, we report the genome sequence of the megaplasmid-bearing Staphylococcus sciuri strain B9-58B, isolated from retail pork. This strain contains a 2,761,440-bp chromosome and a 162,858-bp megaplasmid. The genome contains putative genes involved in virulence, the stress response, and antimicrobial agent and heavy metal resistance.

Here, we present data on the complete genome sequences of 11 Staphylococcus sp. isolates (three S. chromogenes isolates and one isolate each of S. saprophyticus, S. xylosus, S. hominis, S. agnetis, S. caprae, S. aureus, and S. warneri), obtained as part of a mastitis study of buffalo milk (from healthy animals and from those with subclinical mastitis) and milkers’ hands.