Research Publications (Food Safety)

Journal of Applied Microbiology, Accepted Article. Aim This study was done to determine the effects of temperature, pH, and sodium chloride (NaCl) on antimicrobial activity of magnesium oxide (MgO) nanoparticles (NPs) against Escherichia coli O157:H7. Methods and Results Culture conditions were established by varying the pH (5.0, 7.2, and 9.0), NaCl concentration (0.5, 2.0, 3.5, and 5.0%, w/v), and incubation temperatures (4, 12, 22, and 37°C).

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1467-1478, February 2022. Aims The biological events occurring during human digestion help to understand the mechanisms underlying the dose–response relationships of enteric bacterial pathogens.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 290-297, January 2022. Aims In this study, the efficacy of using vacuumed hydrogen peroxide vapour (VHPV) to inactivate foodborne pathogens in whole dried black pepper (Piper nigrum) and powdered dried red pepper (Capsicum annuum) was evaluated. Methods and Results Black and red pepper inoculated with Escherichia coli O157:H7 and Salmonella Typhimurium were su

Aims The purpose of this study was to investigate the occurrence of E. coli O157 and O26 on Belgian dairy cattle farms, the presence of virulence genes in the confirmed isolates, and the association of E. coli O26 presence with calf diarrhea. Methods and Results In total, 233 recto‐anal mucosal swabs (RAMS) were obtained from healthy and diarrheic dairy calves on three farms, each alternately visited three consecutive times. RAMS were analyzed for presence of E.

Aims The goal was to explore the effects of subinhibitory concentration (SIC) (0.5 MIC=20 µg L‐1) of ciprofloxacin on the transcriptome of enterohemorrhagic Escherichia coli O26:H11 (EHEC) isolate by 60 minutes of exposure. Materials and Results We used a combination of comparative genomic and transcriptomic (RNAseq) analyses. The whole genome of the E. coli O26:H11 #30934 strain of bovine origin was sequenced and assembled.

Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7 remains the most frequent serotype associated with disease.

Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E. coli O26, a wild‐type strain and a clinical ATCC strain were used in both steps.

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First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using UV‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate subsequent survival and persistence of cells in simulated gastric fluid (SGF).

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The purpose of this survey was to estimate the respective prevalence of the “gang of seven” and “non-gang of seven” serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014.

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To determine the fate of E. coli on vegetables that were processed through commercial wash treatments and stored under simulated retail conditions at 4°C or wholesale at fluctuating ambient temperatures (0-25°C, dependent on season).

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To evaluate the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top 7 Shiga toxin-producing E. coli (STEC) (O157:H7, O26, O45, O103, O111, O121, and O145) in ground beef and both STEC and Salmonella in co-inoculated samples.

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The occurrence of virulence markers, serotypes and invasive ability were investigated in Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of healthy dairy cattle at Rio de Janeiro State, Brazil.

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The aim of this research was to determine the ability of non-O157 Shiga toxin-producing E. coli (STEC) serogroups to survive with exposure to synthetic gastric fluid (SGF) after adaptation to pineapple juice (PJ) at room and refrigerated temperatures compared to E. coli O157:H7 and to examine the relative transcriptional expression of acid resistance (AR) genes, rpoS, gadA, and adiA.