Research Publications (Food Safety)

Phenolic extract from patawa fruits was encapsulated into acetylated cashew gum polysaccharides and chitosan nanoparticles. acCGP/CS@PEP nanoparticles presented improved antioxidant and antibacterial properties against foodborne pathogens. Summary One recent trend in the food industry is using natural antioxidants and antimicrobials as additives for developing multi‐functional packaging.

Shiga toxin-producing and Enteropathogenic Escherichia coli are foodborne pathogens commonly associated with diarrheal disease in humans. This study investigated the presence of STEC and EPEC in 771 dairy cattle fecal samples which were collected from 5 abattoirs and 9 dairy farms in South Africa. STEC and EPEC were detected, isolated and identified using culture and PCR.

This study investigated the impact of feeding 17% moringa leaf meal (MLM) on the ruminal and fecal microbial composition and body weight gain (BWG) performance of lambs (Ovis aries) and kids (Capra hircus). A total of n = 28 lambs (n = 14, no-moringa, n = 14, 17% moringa) and 24 kids (n = 12, no-moringa, n = 12, 17% moringa) were involved in the experiment and body weight was recorded fortnightly.

IntroductionThis study investigated the interaction with membrane mimetic systems (LUVs), bacterial membranes, the CD spectra, and the bactericidal activity of two designed trematocine mutants, named Trem-HK and Trem-HSK.

In this study, we investigated the antibacterial activity of octyl gallate (OG), an antioxidant food additive, against both Gram-positive and Gram-negative bacterial pathogens. OG demonstrated robust bactericidal activity against Gram-positive bacterial pathogens with minimum inhibitory concentrations (MIC) of 4 to 8 µg/mL and minimum bactericidal concentrations (MBC) of 8 to 16 µg/mL in vitro.

by Mollie Virgo, Serge Mostowy, Brian T. Ho

IntroductionCape gooseberry (Physalis peruviana L.) is a wellconsumed crop in Ecuador, whose fruits are abundant in bioactive molecules.

This study represents the first analysis of the bacterial community in chickens affected by swollen head syndrome, utilizing 16S rRNA gene sequencing. Samples were obtained from clinical laying chickens and were examined for the presence of Avibacterium paragallinarum (APG) and Ornithobacterium rhinotracheale (ORT) using conventional polymerase chain reaction (PCR).

The antibacterial potential of RBPH‐B and RBPH‐C, along with their fractions, is effective against Listeria monocytogenes. Conversely, both SPH‐B and SPH‐C, and their fractions, exhibit pronounced antibacterial activities against Escherichia coli O157:H7 and L. monocytogenes. Notably, the peptide fraction smaller than 10 kDa (SPH‐B‐F3), manifests the highest antimicrobial activities against both bacterial strains.

WHO (World Health Organization) reports from recent years warn about the growing number of antibiotic-resistant bacterial strains. Therefore, there is an urgent need to constantly search for new substances effective in the fight against microorganisms. Plants are a rich source of chemical compounds with antibacterial properties. These compounds, classified as secondary metabolites, may act independently or support the action of currently used antibiotics.

The targeted depletion of potential gut pathogens is often challenging because of their intrinsic ability to thrive in harsh gut environments. Earlier, we showed that Campylobacter jejuni (C. jejuni) exclusively uses the Type-VI Secretion System (T6SS) to target its prey such as Escherichia coli (E. coli), and phenotypic differences between T6SS-negative and T6SS-positive C.

Anthropogenic carbon emissions have resulted in drastic oceanic changes, including increased acidity, increased temperature, and decreased salinity. Anthropogenic carbon emissions have resulted in drastic oceanic changes, including increased acidity, increased temperature, and decreased salinity. Few studies have directly assessed the compounded impact of alterations to oceanic conditions on oyster physiology and the relation to the presence of V. parahaemolyticus.

The formation of ammonia and BAs by microorganisms was monitored using histidine decarboxylase broth (HDB). The use of garlic extract resulted significant inhibition on histamine and tyramine production by migroorganisms.

by Cecilia Rodriguez, Dema Ramlaoui, Briea Gasca, Adiba Azis, Camila Leal, Christina Lopez, Vyanka Merzcord, Kirsten S. McManus, Jasmin Jo, Silvia I. Cazorla, Tomás Subils, Marisel R. Tuttobene, Nicholas T. Salzameda, Robert A. Bonomo, Luis A. Actis, Raúl Raya, María Soledad Ramirez

Plant-parasitic nematodes (PPNs) are among the most damaging pathogens to host plants. Plants can modulate their associated bacteria to cope with nematode infections. The tritrophic plant–nematode–microbe interactions are highly taxa-dependent, resulting in the effectiveness of nematode agents being variable among different host plants. Ficus tikoua is a versatile plant with high application potential for fruits or medicines.

Background Campylobacter (C.) jejuni is one of the most important bacterial foodborne pathogens worldwide. Probiotics such as Lactobacillus or Bacillus species are considered one option for reducing the colonization rate and magnitude in poultry, the most frequent source of human infections. Due to the lack of suitable avian in vitro models such as chicken intestinal cell lines, especially those derived from the cecum, most in vitro studies on C.

Salmonella and Vibrio cholerae are the deadliest foodborne pathogens, requiring effective routine screening for food safety assurance. Hence, a duplex polymerase chain reaction integrated with a lateral flow immunoassay (PCR-LFIA) was developed for rapid detections of Salmonella and V. cholerae to overcome the laborious, time-consuming, complicated, and costly limitations of standard bacterial culture.

The development of sustainable alternatives to conventional antimicrobials is needed to address bacterial virulence while avoiding selecting resistant strains in a variety of fields, including human, animal, and plant health. Quorum sensing (QS), a bacterial communication system involved in noxious bacterial phenotypes such as virulence, motility, and biofilm formation, is of utmost interest.

Tree nuts have been associated with several foodborne outbreaks and recalls in the U.S. While thermal decontamination methods can inactivate pathogens, they come with challenges of varying severity on the product quality. Thus, this study evaluated the effects of nonthermal intense pulsed light (IPL) on Salmonella Typhimurium inactivation and quality attributes of pecan halves.

Vibrio parahaemolyticus is a food-borne pathogen that poses a serious threat to seafood safety and human health. An efficient, nontoxic, and sustainable disinfection material with a stable structure is urgently needed. Herein, silver (Ag)-hydroxyapatite (HAP) composite catalysts were prepared using HAP derived from waste fish bones. The Ag2.50%-HAP showed a 100% disinfection rate against V.

The wet market poultry processing is a concern of spreading microbial pathogens into the environment or to the products. So, this study evaluated the microbiological contamination in poultry settings, i.e., slaughter and processing lines of wet market poultry shops, and the use of nonchlorine sanitizer to improve the sanitation.

The continued worldwide increase in pet ownership has significantly boosted the growth of the pet food industry accompanied by new food safety risks and challenges. This study was designed to determine the occurrence and molecularly characterize multidrug‐resistant (MDR) Enterobacterales in pet food. Eighty‐six (86) packages of dry and wet pet food purchased in different retail stores were screened for carbapenem‐resistant Enterobacterales (CRE).

Abstract Diseases caused by pathogens commonly occurring in the aquatic environment or those that are non‐host specific are prevalent and threaten the rapid growth of tropical aquaculture. This study investigates causes of mortality in 12 batches of newly stocked juvenile Lates calcarifer from three different hatcheries.

BackgroundPork processing plants in the United States (US) cease operations for 24–48 h every six or twelve months to perform intense sanitization (IS) using fogging, foaming, and further antimicrobial treatments to disrupt natural biofilms that may harbor pathogens and spoilage organisms.

by Aaron Embry, Nina S. Baggett, David B. Heisler, Addison White, Maarten F. de Jong, Benjamin L. Kocsis, Diana R. Tomchick, Neal M. Alto, Don B. Gammon Arboviruses are a diverse group of insect-transmitted pathogens that pose global public health challenges.