Research Publications (Food Safety)

Weller, Daniel et al. Environmental (i.e., meteorological and landscape) factors and management practices can affect the prevalence of foodborne pathogens in produce production environments. This study was conducted to determine the prevalence of Listeria monocytogenes, Listeria species (including L.

The O157:H7 (EcO157) epidemiology of Shiga-toxin-producing Escherichia coli (STEC) in cattle is complex, and myths about pre-harvest control are perpetuated. The objectives of this project were to identify perpetuated misinformation and inform four audiences about evidence-based risks and pre-harvest control of EcO157 by addressing: (i) EcO157 epidemiology and pre-harvest control; (ii) how food safety policy is created; and (iii) how to present accurate information about EcO157.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

e-First Articles.

Publication date: 2 September 2015
, Volume 208
Author(s): Stefanie Delbeke , Siele Ceuppens , Liesbeth Jacxsens , Mieke Uyttendaele

An increase in the number of cases of Shiga toxin-producing Escherichia coli (STEC) O157 phage type 2 (PT2) in England in September 2013 was epidemiologically linked to watercress consumption. Whole-genome sequencing (WGS) identified a phylogenetically related cluster of 22 cases (outbreak 1). The isolates comprising this cluster were not closely related to any other United Kingdom strain in the Public Health England WGS database, suggesting a possible imported source.

Publication date: 17 August 2015
, Volume 207
Author(s): Dong Chen , Tong Zhao , Michael P. Doyle

Paoli, George C. et al. Shiga toxin–producing E. coli (STEC) is an important group of foodborne pathogens in the United States and worldwide. Nearly half of STEC-induced diarrheal disease in the United States is caused by serotype O157:H7, while non-O157 STEC account for the remaining illnesses. Thus, the U.S.

Anderson, Kimberly M. et al. PCR-based typing methods, such as repetitive sequence–based PCR (rep-PCR), may facilitate the identification of Shiga toxin–producing Escherichia coli (STEC) by serving as screening methods to reduce the number of isolates to be processed for further confirmation.

Park, Yoen Ju et al. Biofilms are a mixture of bacteria and extracellular products secreted by bacterial cells and are of great concern to the food industry because they offer physical, mechanical, and biological protection to bacterial cells.

Swartz, Richard S. et al. Thermal inactivation of Shiga toxin–producing Escherichia coli (STEC) cells within knitted/cubed beef steaks following cooking on a nonstick griddle was quantified. Both faces of each beef cutlet (ca. 64 g; ca. 8.5 cm length by 10.5 cm width by 0.75 cm height) were surface inoculated (ca.

Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen that may be responsible for severe human infections. Only a limited number of serotypes, including O26:H11, are involved in the majority of serious cases and outbreaks. The main virulence factors, Shiga toxins (Stx), are encoded by bacteriophages. Seventy-four STEC O26:H11 strains of various origins (including human, dairy, and cattle) were characterized for their stx subtypes and Stx phage chromosomal insertion sites.

Escherichia coli O26 has been identified as the most common non-O157 Shiga toxin-producing E. coli (STEC) serogroup to cause human illnesses in the United States and has been implicated in outbreaks around the world. E. coli has high genomic plasticity, which facilitates the loss or acquisition of virulence genes. Attaching and effacing E.

Shiga toxin producing Escherichia coli O157:H7 (STEC) is one of the leading causes of food-poisoning around the world. Some STEC strains produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2) or variants of either toxin, which are critical for the development of hemorrhagic colitis (HC) or hemolytic uremic syndrome (HUS). Currently, there are no therapeutic treatments for HC or HUS. E.

Shiga toxin-producing Escherichia coli (STEC) is an enteropathogen of public health concern because of its ability to cause serious illness and outbreaks. In this prospective study, a diagnostic screening algorithm to categorize STEC infections into risk groups was evaluated. The algorithm consists of prescreening stool specimens with real-time PCR (qPCR) for the presence of stx genes.

Following a large outbreak of Shiga toxin-producing Escherichia coli (STEC) O157 infection in 2012, the prerequisite program for the production of pickles in Japan was revised to include disinfection with sodium hypochlorite (NaClO). Here, we examined the indicator bacterial counts, incidence of STEC and Salmonella spp.

Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk.

Livezey, Kristin W. et al. Escherichia coli O157:H7 and six serovars (O26, O103, O121, O111, O145, and O45) are frequently implicated in severe clinical illness worldwide. Standard testing methods using stx, eae, and O serogroup–specific gene sequences for detecting the top six non-O157 STEC bear the disadvantage that these genes may reside, independently, in different nonpathogenic organisms, leading to false-positive results.

To the Editor: Shigella sonnei causes a bacillary dysentery called shigellosis. Shiga toxins 1 (Stx1) and 2 (Stx2) are mainly produced by Shiga toxin–producing Escherichia coli (STEC), but Stx1 can also be produced by S. dysenteriae serotype 1 (1).

Publication date: 2 July 2015
, Volume 204
Author(s): Yun Jiang , Shuang Yin , Edward G. Dudley , Catherine N. Cutter

Publication date: August 2015
, Volume 49
Author(s): Christopher Sommers , Kathleen T. Rajkowski , O. Joseph Scullen , Jennifer Cassidy , Pina Fratamico , Shiowshuh Sheen

Publication date: Available online 5 March 2015

Author(s): Adriana Albanese , Elizabeth Gerhardt , Hugo García , Natalia Amigo , Angel Cataldi , Elsa Zotta , Cristina Ibarra

Publication date: September 2015
, Volume 50
Author(s): Jinling Shen , Lydia Rump , Wenting Ju , Jingdong Shao , Shaohua Zhao , Eric Brown , Jianghong Meng

Publication date: April 2015
, Volume 111
Author(s): Insook Son , Rachel Binet , Andrew Lin , Thomas S. Hammack , Julie A. Kase
To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors ( stx 1, stx 2, eae , and ehxA ) plus the O157:H7-specific + 93 uidA single nucleotide polymorphism.

Aim
The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge.