Research Publications (Food Safety)

ABSTRACT A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a diverse array of physiological processes in Y. enterocolitica.

The sugar glider (Petaurus breviceps) is a small, arboreal, nocturnal, gliding mammalian possum belonging to the marsupial infraclass. Exotic marsupials, including sugar gliders, are becoming popular companion pets and, consequently, the risk of potential infections that can be transmitted to humans should be investigated. Data on the role of the sugar glider as a possible carrier of pathogenic and zoonotic bacteria are scarce and fragmentary.

Author(s): Cecilia Lucero Estrada, Gabriela Isabel Favier, María Esther Escudero

Aims This study investigated the efficacy of Hydrogen Peroxide Vapour (HPV) at inactivating Hazard Group 3 bacteria that have been presented dried from their growth medium to present a realistic challenge.

To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also important for bacterial survival within host tissues.

In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae.

Author(s): Julia Reichel, Corinna Kehrenberg, Carsten Krischek

Author(s): Karolin Leibiger, Jonas Malte Schweers, Monika Schütz

Author(s): Erwin Bohn, Michael Sonnabend, Kristina Klein, Ingo B. Autenrieth

To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also important for bacterial survival within host tissues.

In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae.

Journal of Food Protection, Volume 82, Issue 10, Page 1683-1687, October 2019.

Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz Banaei

Many pathogenic bacteria use the type III secretion system (T3SS) injectisome to manipulate host cells by injecting virulence-promoting effector proteins into the host cytosol. The T3SS is activated upon host cell contact, and its activation is accompanied by an arrest of cell division; hence, many species maintain a T3SS-inactive sibling population to propagate efficiently within the host.

The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment.

, Ahead of Print.

Xiaojie Jiang, Riikka Keto-Timonen, Mikael Skurnik, Hannu Korkeala

Maria Kusmierek, Jörn Hoßmann, Rebekka Witte, Wiebke Opitz, Ines Vollmer, Marcel Volk, Ann Kathrin Heroven, Hans Wolf-Watz, Petra Dersch

Yersinia enterocolitica is a major agent of foodborne diseases worldwide. Prophage plays an important role in the genetic evolution of the bacterial genome. Little is known about the genetic information about prophages in the genome of Y. enterocolitica, and no pathogenic Y. enterocolitica prophages have been described. In this study, we induced and described the genomes of six prophages from pathogenic Y. enterocolitica for the first time.

Katharina Loreck, Sylvia Mitrenga, Diana Meemken, Regina Heinze, Annett Reissig, Elke Mueller, Ralf Ehricht, Claudia Engemann, Matthias Greiner

Plesiomonas shigelloides is a Gram-negative, flagellated, rod-shaped, ubiquitous, and facultative anaerobic bacterium. It has been isolated from various sources, such as freshwater, surface water, and many wild and domestic animals. P. shigelloides is associated with diarrheal diseases of acute secretory gastroenteritis, an invasive shigellosis-like disease, and a cholera-like illness in humans. At present, 102 somatic antigens and 51 flagellar antigens of P.

Contamination of romaine lettuce with human pathogens, antibiotic-resistant bacteria (ARB), and antibiotic resistance genes (ARGs) occurs during production. Post-harvest interventions are emplaced to mitigate pathogens, but could also mitigate ARB and ARGs on vegetables. The objective of this research was to determine changes to lettuce phyllosphere microbiota, inoculated ARB, and the resistome (profile of ARGs) following washing with a sanitizer, gamma irradiation, and cold storage.

Publication date: Available online 16 March 2019

Source:

Author(s): Heba A. Ahmed, Asmaa B.M.B. Tahoun, Rasha M.M. Abou Elez, Marwa I. Abd El-Hamid, Samah S. Abd Ellatif

Publication date: Available online 15 March 2019

Source: LWT

Author(s): Abdus Sobhan, Jinyoung Lee, Mi-Kyung Park, Jun-Hyun Oh