Research Publications (Food Safety)

Foodborne illness caused by Salmonella spp. is one of the most prevalent public health problems globally, which have brought immeasurable economic burden and social impact to countries around the world. Neither current nucleic acid amplification detection method nor standard culture method (2–3 days) are suitable for field detection in areas with a heavy burden of Salmonella spp.

Human listeriosis is a foodborne disease caused by Listeria monocytogenes contamination. Most cases occur due to consumption of ready-to-eat (RTE) foods. Stewed meat in seasoning, a type of pre-packaged ready-to-eat (RTE) food, is a popular traditional product in China. We used whole genome sequencing to analyze core genome multilocus sequence typing (cgMLST), serogroups and multilocus typing sequence typing (ST) of 67 L.

A novel temperate phage, named Hesat, was isolated by the incubation of a dairy strain of Staphylococcus aureus belonging to spa-type t127 with either bovine or ovine milk.

Heat resistance, D- and z-values of five Salmonella enterica strains, namely S. Senftenberg LTH 5703, S. Typhimurium ATCC 13311 and ATCC 14028, S. Saintpaul LTH 6494, and S. Enteritidis ATCC 13076, as well as of a Salmonella cocktail and three potential surrogate strains were investigated in phosphate buffered saline and strawberry nectar (12° Brix).

A novel loop‐mediated isothermal amplification assay was established on the basis of screening and verification of detection targets for rapid detection of Staphylococcus aureus. After amplification, there are two distinct methodologies for result determination: one involves the analysis of fluorescence curves, while the other involves the observation of color changes.

This research study the effect of the food product, even present in trace in the bacteria's habitat, on the bacteria potential of biofilm formation. Understanding the conditions that favor or disadvantage the formation of biofilms could help in the development and implementation of new alternative strategies to combat food‐borne infections.

Abstract This study investigated the disparities in soil characteristics and pathogenic bacteria prevalence between shrimp ponds affected by acute hepatopancreatic necrosis disease (AHPND) and unaffected ponds, alongside examining the spatial distribution of soil attributes in flat‐oriented pond soil strata.

Kefir is a fermented dairy product based on the fermentation of milk by bacteria and yeasts. It is produced by adding kefir grains, consisting of a consortium of microorganisms, to milk in order to start a natural fermentation (Garofalo et al., 2020).

Campylobacter jejuni represents one of the leading causes of bacterial gastroenteritis in humans and is primarily linked to chicken meat contamination. In the present study, we analyzed the virulence and survival genes, antimicrobial resistance, and the clonal distribution of 50 C. jejuni isolates obtained from various sources in 14 chicken slaughterhouses across 8 provinces in South Korea from 2019 to 2022.

Salmonella enterica serovar Typhimurium (S. Typhimurium), a foodborne pathogen that poses significant public health risks to humans and animals, presents a formidable challenge due to its antibiotic resistance. This study explores the potential of Lactobacillus acidophilus (L. acidophilus 1.3251) probiotics as an alternative strategy to combat antibiotic resistance associated with S. Typhimurium infection.

Campylobacter jejuni (C. jejuni) is a common pathogen that often causes diarrhea, loss of appetite, and even enteritis in domestic cats, affecting their growth and development, especially in kittens under 6 months of age. Oral passive immunization with chicken yolk antibody Y has been proved effective for the treatment of gastrointestinal pathogen infections due to its high specificity. In this study, C.

Staphylococcus species are the primary cause of mastitis in dairy cows across the world. Staphylococcus aureus has recently become a pathogen that is zoonotic and multidrug resistant. This study aimed to sequence whole genomes of 38 S. aureus isolates from 55 subclinical mastitis dairy cows of 7 small-scale farmers in the Free State Province, South Africa and document and their antimicrobial and virulence genes.

Camel milk is highly nutritious but contamination with biofilm forming methicillin resistant Staphylococcus aureus (MRSA) may pose a serious threat to human health and food safety. Therefore, sinapic acid (SA) was assessed for its in vitro antibiofilm and antivirulence potential against MRSA isolated from raw camel milk samples.

The rumen microbiota is important for energy and nutrient acquisition in cattle, and therefore its composition may also affect carcass merit and meat quality attributes.

Antimicrobial-resistant bacteria (ARB) from the intestinal contents of wild fish may have a relevant ecological significance and could be used as indicators of antimicrobial-resistance dissemination in natural bacterial populations in water bodies impacted by urban contamination. Thus, the occurrence of ARB in the intestinal contents of pelagic and demersal wild fishes captured in anthropogenic-impacted Coquimbo Bay in Chile was studied.

The development of a simple, rapid, sensitive, and accurate method for detecting Escherichia coli O157:H7 in complex matrices is critical for ensuring food safety. In this study, a fluorescent biosensor employing aptamer (Apt) -functionalized magnetic beads and cascade strand displacement amplification (SDA) was devised for the detection of E. coli O157:H7 in milk samples. E.

Campylobacter spp., such as Campylobacter jejuni and Campylobacter coli, are important zoonotic Gram-negative pathogens that cause acute intestinal diseases in humans. In this study, a retrospective analysis was conducted on previously collected Campylobacter isolates from antimicrobial resistance surveillance. A total of 29 optrA-positive C.

The present study aimed to assess the occurrence and counts of Staphylococcus aureus in Brazilian artisanal cheeses (BAC) produced in five regions of Brazil: Coalho and Manteiga (Northeast region); Colonial and Serrano (South); Caipira (Central-West); Marajó (North); and Minas Artisanal cheeses, from Araxá, Campos das Vertentes, Cerrado, Serro and Canastra microregions (Southeast).

The interaction between environmental factors and Vibrio in bivalves is not well understood, despite the widely held belief that pathogen infection and seawater temperature significantly impact summer mortality. In the present study, we conducted simulated experiments to explore the effects of high temperature and Vibrio infection on the clam Meretrix petechialis.

This study investigated the effects of defective pear fermentation (DPF) diets on growth performance and gastrointestinal microbial communities in 60 healthy male small-tailed Han sheep, aged 90 days. The sheep were randomly divided into four groups, each consisting of three replicates with five sheep per replicate. Initially, all groups received a basal diet for seven days during the adaptation stage.

Combined antibacterial effect of Origanum essential oils and antibiotics against multiresistant foodborne Salmonella in Morocco. Abstract The present study aims to assess the antibacterial activity of endemic Moroccan Origanum essential oils (Origanum elongatum and Origanum compactum) alone and in association with antibiotics against multidrug resistant foodborne Salmonella. The well diffusion and microdilution procedures were used to evaluate the antibacterial activity.

We demonstrated for the first time that Salmonella enterica Typhimurium can form dry surface biofilms (DSB) on surfaces. Mean reduction in viable cells recovered from Salmonella Typhimurium DSB following treatment with water (A and C) or the most efficacious disinfectant formulation (B and D) tested from a panel of candidates, with or without a subsequent wiping step. Contact times used were as per manufacturer's instructions.

Bacillus cereus is a foodborne pathogen that widely exists in various food products. In this study, a new B. cereus phage, named Z3, was isolated from the soil and characterized. Transmission electron microscopy (TEM) observations revealed that Z3 had an icosahedral head and a retractable tail, belonging to myxovirus. The double-stranded linear DNA of Z3 was 159,116 bp in length with a CG% content of 39.96%.

The pork production chain is an important reservoir of antimicrobial resistant bacteria. This study identified and characterized integrons in Salmonella isolates from a Brazilian pork production chain and associate them with their antibiotic resistance pattern. A total of 41 whole-genome sequencing data of nontyphoidal Salmonella were analyzed using PlasmidSPAdes and IntegronFinder software.