Research Publications (Food Safety)

This study investigates the antimicrobial activity of insect chitosan against vegetative cells of Bacillus cereus in a rice matrix. Sample culture solutions were prepared with different concentrations of insect chitosan (150, 180, 220 and 250 μg/mL) and tested at three temperatures (30 °C, 20 °C and 10 °C), which simulate different storage temperature scenarios of precooked rice.

Bacillus cereus, an important foodborne pathogen, poses a risk to food safety and quality. Robust biofilm formation ability is one of the key properties that is responsible for the food contamination and food poisoning caused by B. cereus, especially the emetic strains. To investigate the mechanism of biofilm formation in emetic B. cereus strains, we screened for the mutants that fail to form biofilms by using random mutagenesis toward B.

Mercury (Hg) pollution is a worldwide problem and increasing day by day due to natural and anthropogenic sources. In this study, mercury-resistant (HgR) bacterial isolates were isolated from industrial wastewater of Ittehad Chemicals Ltd., Kala Shah Kaku, Lahore, Pakistan. Out of 65 bacterial isolates, five isolates were screened out based on showing resistance at 30–40 μg/ml against HgCl2.

Microbiology Resource Announcements, Ahead of Print. Polyhydroxyalkanoates (PHA) are microbially produced biopolymers which are biodegradable and biocompatible. These compounds produced by microorganisms have been described as a potent alternative to synthetic plastics, which are often recalcitrant. Here, we report the draft genome sequence of a PHA-producing Bacillus cereus isolated in our laboratory.

Microbiology Resource Announcements, Volume 11, Issue 5, May 2022. Bacillus cereus strain CPT56D-587-MTF was isolated from nitrate- and toxic metal-contaminated subsurface sediment at the Oak Ridge Reservation (ORR) (Oak Ridge, TN, USA). Here, we report the complete genome sequence of this strain to provide genomic insight into its strategies for survival at this mixed-waste site.

Clinical Microbiology Reviews, Volume 35, Issue 2, April 2022. Bacillus cereus group species are widespread, Gram-positive, spore-forming environmental bacteria. B. cereus sensu stricto is one of the major causes of food poisoning worldwide. In high-risk individuals, such as preterm neonates, B. cereus infections can cause fatal infections.

Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. The infection of a bacterium by a tailed phage starts from the adsorption process, which consists of a specific and strong interaction between viral proteins called receptor binding proteins (RBPs) and receptors located on the bacterial surface.

In this study, eight rap-related genes were found in the Bacillus cereus 0–9 genome; five rap genes were located on chromosomes and three on large plasmids. A total of five Rap proteins in B. cereus 0–9 were annotated as ‘tetratricopeptide repeat proteins’. SMART Server analysis showed that the eight Rap proteins had typical tetrapeptide repeat sequence (TPR) domains.

The aim of this study was to evaluate the effect of Bacillus cereus (B. cereus) on the seedling growth and accumulation of medicinal ingredients of Glycyrrhiza uralensis Fisch. (G. uralensis) under control and salt stress conditions. Our results revealed the different effects of B. cereus on the seedling growth and accumulation of medicinal ingredients particularly in different conditions based on the transcriptome and polymerase chain reaction (PCR) analysis. Under the control condition, B.

Journal of Applied Microbiology, Accepted Article. Aims Bacillus cereus is often responsible for foodborne diseases and both local and systemic infections in humans. Cases of infection in other mammals are rather rare. In this study, we report a B.

Rapid detection of antibiotic resistance genes (ARGs) of food microorganisms is critical for preventing the spread of ARGs via the food chain. In this study, slipchip-based immunomagnetic separation (IMS) combined with loop-mediated isothermal amplification (LAMP) was developed for rapid, specific, sensitive and multi-sample detection of Bacillus cereus with tetracycline resistance gene tetL in pasteurized milk.

To combat the public health threat posed by multiple-drug-resistant (MDR) pathogens, new drugs with novel chemistry and modes of action are needed.

Antimicrobial resistance (AMR) has been a leading issue for human health globally threatening the achievement of several of the Sustainable Development Goals (SDGs). Originated from Bacillus cereus, carbapenemases phenotype has been considered to be a major concern in AMR. In this study, the AMR identification rate of P. aeruginosa isolates and infections in FAHJU showed an obvious upward trend from 2012 to 2016. All 88 carbapenem-resistant P.

As natural biopolymers, chitosan and lignin are characterized by their good biocompatibility, high biodegradability and satisfactory biosafety. The active polymers’ functional groups are responsible for the potential of these biomaterials for use as carrier matrices in the construction of polymer–drug conjugates with prospective applicability in the fields of medicine, food and agriculture—subjects that have attracted attention in recent years.

The buffalo is an amazing ruminant. Its ability to degrade lignin, which has been recently reported, is most likely due to unique rumen microorganisms with lignin-degradation potential. Our goal was to explore the lignin-degradation potential of ruminal microorganisms, in which ligninolytic enzyme encoding genes were involved to provide ideas for revealing the mechanism of lignin degradation by buffalo.

This study was conducted to investigate the antibacterial activity and mechanism of (4-[(4′-O-acetyl-α-L-rhamnosyloxy)benzyl] isothiocyanate) (RBITC) from Moringa oleifera seeds at the molecular level against Bacillus cereus (B. cereus) and Cronobacter sakazakii (C. sakazakii) and to further analyze the application of RBITC in goat milk.

Heavy metal resistant bacteria play an important role in the metal biogeochemical cycle in soil, but the benefits of microbial oxidation for plants and soil have not been well-documented. The purpose of this study was to explore the contribution of two Bacillus spp. to alleviate the antimony (Sb) toxicity in plants, and, then, to propose a bioremediation method for Sb contaminated soil, which is characterized by environmental protection, high efficiency, and low cost.

Emetic Bacillus cereus (B. cereus) is a foodborne microorganism associated with vomiting disease. Herein, we have established a blocker-tailed polymerase chain reaction (bt-PCR) coupled with rolling circle amplification (RCA) assay for the sensitive and specific detection of emetic B. cereus.

Root-knot nematodes (RKNs) are soil-borne pathogens that severely affect Panax notoginseng growth and productivity. Thus, there is an urgent need for biological control agents or green nematicides to control root-knot nematodes. Rhizosphere bacteria can effectively control RKNs through different mechanisms. In this study, the three rhizosphere Bacillus strains, isolated from the root of P.

by Ravinder Polapally, Manasa Mansani, Karthik Rajkumar, Sandeepta Burgula, Bee Hameeda, Alaa Alhazmi, Farkad Bantun, Atiah H. Almalki, Shafiul Haque, Hesham Ali El Enshasy, R. Z. Sayyed The present study reveals the production of dark, extracellular melanin pigment (386 mg/L) on peptone yeast extract iron agar medium by Streptomyces puniceus RHPR9 using the gravimetric method.

Endophytic strains belonging to the Bacillus cereus group were isolated from the halophytes Atriplex halimus L. (Amaranthaceae) and Tamarix aphylla L. (Tamaricaceae) from costal and continental regions in Algeria. Based on their salt tolerance (up to 5%), the strains were tested for their ability to alleviate salt stress in tomato and wheat. Bacillus sp.

In the last decade, foodborne outbreaks and individual cases caused by bacterial toxins showed an increasing trend. The major contributors are enterotoxins and cereulide produced by Bacillus cereus, which can cause a diarrheal and emetic form of the disease, respectively. These diseases usually induce relatively mild symptoms; however, fatal cases have been reported.

The electrochemical immunosensor is one of the most promising detection approaches for pathogenic microorganism. In this study, a label-free electrochemical immunosensor based on chitosan/gelatin was fabricated for the detection of Bacillus cereus. Anti-Bacillus cereus polyclonal antibodies were immobilized through a cross-linking method on a chitosan/gelatin-modified electrode.

by Saba Ahmed, Nadeem Iqbal, Xiaoyan Tang, Rafiq Ahmad, Muhammad Irshad, Usman Irshad Functioning of ecosystems depends on the nutrient dynamics across trophic levels, largely mediated by microbial interactions in the soil food web. The present study investigated the use of phosphate solubilizing bacteria (PSB) and poultry manure (PM) for maintaining labile P in the soil for an extensive fertility enhancement and as a substitution of chemical fertilizers.

Chitosan-ZnO nanoparticle (ZnONP) edible coating was applied to extend shelf life of wild-simulated Korean ginseng root (WsKG). In antimicrobial testing of various coating solutions (0.01, 0.02, 0.03% ZnONP), Bacillus cereus (Gram-positive) and Escherichia coli (Gram-negative) were most inhibited by the 0.03% chitosan-ZnONP solution. The 0.03% chitosan-ZnONP solution was finally used for edible coating of WsKG.