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Research Publications (Food Safety)

The Food Safety Publications tracks research that is published across national and international peer-reviewed journals. Recent articles are available ahead of print and searchable by Journal, Article Title, and Category. The research publications are tracked across six categories: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, Produce Safety, and Viruses. Articles produced by USDA Grant Funding Agencies and FDA Grant Funding Agencies are also tracked.

Displaying 1 - 25 of 1142

  1. Effect of Probiotic E. coli Nissle 1917 Supplementation on the Growth Performance, Immune Responses, Intestinal Morphology, and Gut Microbes of Campylobacter jejuni Infected Chickens

    • Fri, 09/23/2022 - 04:00
    • Infection and Immunity
    • Infection and Immunity, Ahead of Print. Campylobacter jejuni is the most common cause of bacterial foodborne gastroenteritis and holds significant public health importance. The continuing increase of antibiotic-resistant Campylobacter necessitates the development of antibiotic-alternative approaches to control infections in poultry and in humans. Here, we assessed the ability of E. coli Nissle 1917 (EcN; free and chitosan-alginate microencapsulated) to reduce C.

      • Bacterial pathogens
      • Campylobacter
  2. Quantitative Determination Of Campylobacter On Broilers Along 22 UK Processing Lines, To Identify Potential Process Control Points and Cross-contamination From Colonized To Uncolonized Flocks.

    • Fri, 09/23/2022 - 04:00
    • As part of a program to reduce numbers of the human pathogen Campylobacter on retail chickens twenty-two broiler processing lines, representing over 90% of United Kingdom (UK) production, were characterized by enumerating Campylobacter on pooled neck skins after the exsanguination, scalding, defeathering, evisceration, crop removal, inside-outside washing, and air-chilling stages of processing.  Sixteen of the processing lines investigated showed significant (p<0.05) reductions in Campylob

      • Bacterial pathogens
      • Campylobacter
  3. Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods

    • Sat, 09/17/2022 - 04:00
    • PLOS ONE
    • by Farina Khattak, Salvatore Galgano, Jos Houdijk The study aimed to delineate the robustness of the culture-based and molecular biology methods to assess the total bacterial concentration and Campylobacter jejuni (C. jejuni) quantification in caecal content, analysed as fresh or after being stored immediately at ultra-low (-80°C) temperature at different time points (for 3, 7, 14, 28 and 62 days post collection).

      • Bacterial pathogens
      • Campylobacter
  4. Complete Genome Sequences of Four Campylobacter jejuni Strains Isolated from Retail Chicken Meat and Broiler Feces

    • Fri, 09/16/2022 - 04:00
    • Microbiology Resource Announcements
    • Microbiology Resource Announcements, Ahead of Print. Campylobacter jejuni is the leading pathogen that causes foodborne infections. Here, we report the complete genome sequences of four C. jejuni strains isolated from retail chicken meat and broiler feces samples. Genes encoding type VI secretion and antibiotic resistance were detected among these isolates.

      • Bacterial pathogens
      • Campylobacter
  5. Molecular diagnosis of bovine genital campylobacteriosis using high-resolution melting analysis

    • Fri, 09/09/2022 - 04:00
    • Frontiers in Microbiology
    • Bovine Genital Campylobacteriosis (BGC) is a worldwide spread venereal disease of cattle caused by Campylobacter fetus subsp. venerealis (Cfv). Although several real-time PCR assays were developed for Cfv identification, most target mobile genetic elements, which may lead to false-positive diagnosis. In this study, a real-time PCR assay coupled with High-Resolution Melting analysis (HRM) was developed for the identification of Campylobacter fetus subspecies and application in BGC diagnosis.

      • Bacterial pathogens
      • Campylobacter
  6. Rhodiola rosea Reduces Intercellular Signaling in Campylobacter jejuni

    • Fri, 09/09/2022 - 04:00
    • Antibiotics
    • Campylobacter jejuni is a major foodborne pathogen and the leading cause of bacterial gastroenteritis, i.e., campylobacteriosis. Besides searching for novel antimicrobials, identification of new targets for their action is becoming increasingly important. Rhodiola rosea has long been used in traditional medicine. Ethanolic extracts from the roots and rhizomes of the plant contain a wide range of bioactive compounds with various pharmacological activities.

      • Bacterial pathogens
      • Campylobacter
  7. Research Note: Campylobacter spp. control at field level two years after the implementation of European Regulation (EU) 2017/1495

    • Fri, 09/09/2022 - 04:00
    • Poultry Science
    • Campylobacteriosis was the most frequently reported foodborne infection in humans in the European Union in the last years. Campylobacter spp. in broiler flocks from Spain was monitored at farm level during 12-month period (2020–2021).

      • Bacterial pathogens
      • Campylobacter
  8. Prevalence of the phenicol resistance gene fexA in Campylobacter isolated from the poultry supply chain

    • Wed, 09/07/2022 - 04:00
    • International Journal of Food Microbiology
    • Florfenicol, an animal-specific broad-spectrum antibiotic, has been widely used in livestock and poultry breeding, which leads to the high antimicrobial resistance (AMR) of Campylobacter in food animals.

      • Bacterial pathogens
      • Campylobacter
  9. Metaphenotypes associated with recurrent genomic lineages of Campylobacter jejuni responsible for human infections in Luxembourg

    • Wed, 09/07/2022 - 04:00
    • Frontiers in Microbiology
    • Campylobacter jejuni is a leading cause of foodborne illnesses worldwide. Although considered fragile, this microaerophilic bacterium is able to survive in various challenging environments, which subsequently constitutes multiple sources of transmission for human infection. To test the assumption of acquiring specific features for adaptation and survival, we established a workflow of phenotypic tests related to the survival and the persistence of recurrent and sporadic strains.

      • Bacterial pathogens
      • Campylobacter
  10. Change of Campylobacter, Escherichia coli and Salmonella counts in packaged broiler breast meat stored under modified atmosphere and vacuum conditions at 4 and 10 °C based on cultural and molecular biological quantification

    • Sun, 09/04/2022 - 04:00
    • Food Control
    • Poultry on the European market is offered primarily as fresh meat and is usually sold at the retail level packed under either a modified atmosphere (MAP) or vacuum. Packaging is meant to retain product quality during retail offering and influences microbiological changes during storage.

      • Bacterial pathogens
      • Campylobacter
      • Salmonella
  11. Host‐associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni

    • Sat, 09/03/2022 - 04:00
    • Zoonoses and Public Health
    • Zoonoses and Public Health, EarlyView. Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection.

      • Bacterial pathogens
      • Campylobacter
  12. Complete Genome Sequence of Campylobacter coli Strain P4581, a Hybrid Carrying Campylobacter jejuni Genomic Content, Isolated from Rhesus Monkey, Macaca mulatta

    • Fri, 09/02/2022 - 04:00
    • Microbiology Resource Announcements
    • Microbiology Resource Announcements, Ahead of Print. Campylobacter coli is a leading bacterial cause of human gastroenteritis. We reported the circularized 1.8-Mbp complete genome of MLST type 1055 C. coli strain P4581 isolated from a rhesus monkey, Macaca mulatta, hybridizing Illumina short- and Nanopore long-reads.

      • Bacterial pathogens
      • Campylobacter
  13. Current methodologies and future direction of Campylobacter isolation and detection from food matrices, clinical samples, and the agricultural environment

    • Wed, 08/31/2022 - 04:00
    • Journal of Microbiological Methods
    • Campylobacter spp. are the leading cause of bacterial foodborne infections in both developed and developing countries. The food commodities primarily attributed to campylobacteriosis include raw milk, poultry, seafood, and fresh produce. Furthermore, insects, animal/bird fecal material, and agricultural water have been shown to be the sources of Campylobacter contamination in these commodities.

      • Bacterial pathogens
      • Campylobacter
  14. Prevalence and Antimicrobial Resistance of Campylobacter spp. Isolated from Broilers Throughout the Supply Chain in Valencia, Spain

    • Tue, 08/30/2022 - 04:00
    • Foodborne Pathogens and Disease
    • Campylobacter is a major foodborne pathogen and its antimicrobial resistance (AMR) has been described worldwide. The main objective of this study was to determine the occurrence and AMR of Campylobacter spp. isolated from broilers throughout the supply chain in Valencia, Spain. A total of 483 samples were included in the analysis: 430 from the slaughterhouse (chicken carcass and neck skin) and 53 from the point of sale (retail broiler and packaging).

      • Bacterial pathogens
      • Campylobacter
  15. In a warm climate, ventilation, indoor temperature and outdoor relative humidity have significant effects on Campylobacter spp. colonization in chicken broiler farms which can occur in only 2 days

    • Mon, 08/29/2022 - 04:00
    • Food Microbiology
    • A longitudinal study was conducted in five conventional broiler farms during a 2-year period to determine the dynamics of Campylobacter infection in a warm climate region (north-eastern Spain). Weekly sampling of 63 flocks was performed upon one-day-old chick placement, including animal and environmental samples. Campylobacter spp. detection was assessed by culture and non-culture methods.

      • Bacterial pathogens
      • Campylobacter
  16. Campylobacter jejuni and Other Emerging Campylobacteraceae in Retail Beef Liver ‐ An Underestimated Potential Source?

    • Thu, 08/25/2022 - 11:11
    • Letters in Applied Microbiology
    • Letters in Applied Microbiology, Accepted Article. Bovine by-products, such as liver, could be an underestimated source of Campylobacter jejuni. Therefore, our aims were to evaluate the occurrence of C.

      • Bacterial pathogens
      • Campylobacter
  17. Two-component regulatory systems in Helicobacter pylori and Campylobacter jejuni: Attractive targets for novel antibacterial drugs

    • Wed, 08/24/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • Two-component regulatory systems (TCRS) are ubiquitous signal transduction mechanisms evolved by bacteria for sensing and adapting to the constant changes that occur in their environment.

      • Bacterial pathogens
      • Campylobacter
  18. Bio-responsive composite liposomes against Campylobacter jejuni in vitro and its application in chicken preservation

    • Wed, 08/24/2022 - 04:00
    • Innovative Food Science & Emerging Technologies
    • The infection of Campylobacter jejuni (C. jejuni) in food can cause huge economic and health costs, and liposomes based on biological switch is a promising antibacterial strategy. In this study, a bio-responsive composite liposome was designed with silk fibroin and L-fucose (FLPs) based on the chemotaxis and protease of C. jejuni, and Litsea cubeba essential oil as antibacterial agent.

      • Bacterial pathogens
      • Campylobacter
  19. Combining enrichment with multiplex real-time PCR leads to faster detection and identification of Campylobacter spp. in food compared to ISO 10272–1:2017

    • Sat, 08/20/2022 - 04:00
    • Food Microbiology
    • Conventional protocols for the detection of Campylobacter from foods are laborious and time-consuming. This research describes an alternative procedure (EMRT-PCR) for the detection of Campylobacter from food by combining ISO 10272–1:2017 enrichment in Bolton broth (BB) with a multiplex real-time (MRT-) PCR assay. Species differentiation was done by targeting C. jejuni (mapA), C. coli (ceuE), and both species (cje).

      • Bacterial pathogens
      • Campylobacter
  20. Molecular markers associated with antimicrobial resistance and genotypes of Campylobacter jejuni, and Campylobacter coli isolated from broiler and swine flocks in southeast Brazil

    • Fri, 08/19/2022 - 04:00
    • Comparative Immunology, Microbiology and Infectious Diseases
    • This study aimed to identify molecular markers associated with antimicrobial resistance and genotype in isolates of Campylobacter spp. isolated from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n=35) strains were screened for the antimicrobial genetic markers C257T in gyrA, A2074C and A2075G in 23 S rRNA, CmeABC, ermB, tetO and blaOXA61 by PCR.

      • Bacterial pathogens
      • Campylobacter
  21. Antibiotic Resistance in Bacteria—A Review

    • Wed, 08/10/2022 - 04:00
    • Antibiotics
    • A global problem of multi-drug resistance (MDR) among bacteria is the cause of hundreds of thousands of deaths every year. In response to the significant increase of MDR bacteria, legislative measures have widely been taken to limit or eliminate the use of antibiotics, including in the form of feed additives for livestock, but also in metaphylaxis and its treatment, which was the subject of EU Regulation in 2019/6.

      • Bacterial pathogens
      • Campylobacter
      • Salmonella
      • Staphylococcus aureus
  22. Longitudinal Changes in Campylobacter and the Litter Microbiome throughout the Broiler Production Cycle

    • Wed, 08/10/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Broiler chickens are an important source of Campylobacter to humans and become colonized on the farm, but the role of the litter in the ecology of Campylobacter is still not clear. The aim of this study was to examine the relationship between Campylobacter and the changes in the litter microbiome throughout the broiler production cycle.

      • Bacterial pathogens
      • Campylobacter
  23. Exploiting Violet-Blue Light to Kill Campylobacter jejuni: Analysis of Global Responses, Modeling of Transcription Factor Activities, and Identification of Protein Targets

    • Fri, 08/05/2022 - 04:00
    • mSystems
    • mSystems, Ahead of Print. Campylobacter jejuni is a microaerophilic foodborne zoonotic pathogen of worldwide concern as the leading cause of bacterial gastroenteritis. Many strains are increasingly antibiotic resistant and new methods of control are required to reduce food-chain contamination. One possibility is photodynamic inactivation (PDI) using violet-blue (VB) light, to which C. jejuni is highly susceptible.

      • Bacterial pathogens
      • Campylobacter
  24. A smartphone-assisted high-throughput integrated color-sensing platform for the rapid detection of Campylobacter coli

    • Thu, 08/04/2022 - 04:00
    • LWT
    • Campylobacter is one of the most common foodborne pathogens worldwide. A new smartphone-assisted high-throughput integrated color-sensing platform, called the HICS platform, was developed for the rapid detection of Campylobacter coli. This platform was based on the visual loop-mediated isothermal amplification method. Using this system, as many as 64 samples could be assessed in less than an hour after enrichment.

      • Bacterial pathogens
      • Campylobacter
  25. The effect of Campylobacter jejuni and Campylobacter coli colonization on the gut morphology, functional integrity, and microbiota composition of female turkeys

    • Thu, 08/04/2022 - 04:00
    • Gut Pathogens
    • Background Campylobacter (C.) species are the most common bacterial cause of foodborne diarrhea in humans. Despite colonization, most animals do not show clinical signs, making recognition of affected flocks and disruption of the infection chain before slaughter challenging. Turkeys are often cocolonized with C. jejuni and C. coli.

      • Bacterial pathogens
      • Campylobacter