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Research Publications (Food Safety)

The Food Safety Publications tracks research that is published across national and international peer-reviewed journals. Recent articles are available ahead of print and searchable by Journal, Article Title, and Category. The research publications are tracked across six categories: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, Produce Safety, and Viruses. Articles produced by USDA Grant Funding Agencies and FDA Grant Funding Agencies are also tracked.

Displaying 1 - 25 of 285

  1. Modelling the UV-C inactivation kinetics and determination of fluence required for incremental inactivation of Cronobacter spp.

    • Fri, 09/09/2022 - 04:00
    • A study was undertaken to model the UV‑C inactivation kinetics and determine the fluences required for the incremental inactivation of several strains of Cronobacter  spp. suspended in clear phosphate-buffered saline (PBS). A total of 13 strains of Cronobacter  spp. were individually suspended in PBS and treated with UV‑C doses of 0, 2, 4, 6, 8 and 10 mJ cm ‑2 using a collimated beam device emitting UV‑C at 253.7 nm.

      • Bacterial pathogens
      • Cronobacter
  2. Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

    • Fri, 08/19/2022 - 04:00
    • PLOS ONE
    • by Fahad Alanazi, Islam Nour, Atif Hanif, Ibrahim Al-Ashkar, Reem M. Aljowaie, Saleh Eifan The diversity among bacteriophages depends on different factors like ecology, temperature conditions and genetic pool.

      • Bacterial pathogens
      • Cronobacter
  3. Enhancement of Thermal Inactivation of Cronobacter sakazakii in Apple Juice at 58 ºC by Inclusion of Butyl Para-Hydroxybenzoate and Malic Acid.

    • Sat, 08/13/2022 - 04:00
    • After studies with powdered infant formula indicated that the enhancement of thermal inactivation of Cronobacter sakazakii by butyl para-hydroxybenzoate (BPB) was blocked by the presence of high protein levels, we hypothesized that BPB would retain its synergistic activity in foods with a limited protein content and lipid content.

      • Bacterial pathogens
      • Cronobacter
  4. Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

    • Thu, 08/11/2022 - 04:00
    • Antibiotics
    • This study aims to detect the prevalence and antimicrobial resistance of Listeria monocytogenes and Cronobacter sakazakii in three dairy households and dried milk from different suppliers, and evaluate the antimicrobial effect of rose water, rose, and orange essential oils. In total, 360 samples were collected from cattle, the environment, and dried milk (n = 30). Antimicrobial activity was evaluated with twofold microtube dilution and the time-kill method. L.

      • Bacterial pathogens
      • Cronobacter
      • Listeria monocytogenes
  5. Cronobacter spp. in foods of plant origin: occurrence, contamination routes, and pathogenic potential

    • Sun, 07/24/2022 - 04:00
    • Critical Reviews in Food Science and Nutrition
    • Cronobacter is an emerging bacterial pathogen associated with infections such as necrotizing enterocolitis, sepsis, and meningitis in neonates and infants, related to the consumption of powdered infant formula. In addition, this bacterium can also cause infections in adults by the ingestion of other foods. Thus, this review article aims to report the occurrence and prevalence of Cronobacter spp.

      • Bacterial pathogens
      • Cronobacter
  6. Identification and antibiotic resistance of Cronobacter spp. isolated from dried edible mushrooms

    • Thu, 07/21/2022 - 04:00
    • Journal of Food Science
    • Journal of Food Science, EarlyView. Cronobacter spp. is an important foodborne pathogen that can cause life-threatening diseases in infants and immunocompromised adults. The present study was carried out to understand the prevalence and characterization of Cronobacter spp.

      • Bacterial pathogens
      • Cronobacter
  7. Virulence, antimicrobial susceptibility, and phylogenetic analysis of Cronobacter sakazakii isolates of food origins from Jordan

    • Thu, 07/21/2022 - 04:00
    • Journal of Applied Microbiology
    • Journal of Applied Microbiology, Accepted Article. Aims to characterize a collection of Cronobacter sakazakii isolates collected from various origins in Jordan. Methods and Results the isolates were characterized using 16S rRNA sequencing, DNA microarray, multi-locus sequence typing (MLST), O-serotyping, virulence gene identification, and antibiotic susceptibility testing. The identities and phylogenetic relatedness revealed that C.

      • Bacterial pathogens
      • Cronobacter
  8. Environmental risk factors associated with the survival, persistence, and thermal tolerance of Cronobacter sakazakii during the manufacture of powdered infant formula

    • Sun, 07/17/2022 - 04:00
    • Critical Reviews in Food Science and Nutrition
    • Cronobacter sakazakii is an opportunistic foodborne pathogen of concern for foods having low water activity such as powdered infant formula (PIF). Its survival under desiccated stress can be attributed to its ability to adapt effectively to many different environmental stresses. Due to the high risk to neonates and its sporadic outbreaks in PIF, C. sakazakii received great attention among the scientific community, food industry and health care providers.

      • Bacterial pathogens
      • Cronobacter
  9. Inhibition of Cronobacter sakazakii in an infant simulator of the human intestinal microbial ecosystem using a potential synbiotic

    • Fri, 07/15/2022 - 04:00
    • Frontiers in Microbiology
    • Powdered infant formula (PIF) can be contaminated with Cronobacter sakazakii, which can cause severe illnesses in infants. Synbiotics, a combination of probiotics and prebiotics, could act as an alternative control measure for C. sakazakii contamination in PIF and within the infant gut, but synbiotics have not been well studied for their ability to inhibit C. sakazakii.

      • Bacterial pathogens
      • Cronobacter
  10. Silver nanoparticles reduce the tolerance of Cronobacter sakazakii to environmental stress by inhibiting expression of related genes

    • Wed, 07/13/2022 - 08:00
    • Journal of Dairy Science
    • Cronobacter sakazakii is a food-borne pathogen that is resistant to a variety of environmental stress conditions. It can survive in harsh environments. We studied the effects of silver nanoparticles (AgNP) on the environmental tolerance and biofilm formation of C. sakazakii. First, we determined the minimum inhibitory concentration (MIC) of AgNP to C.

      • Bacterial pathogens
      • Cronobacter
  11. Specific detection of viable Cronobacter sakazakii in powdered infant formula by phage amplification combined with qPCR (PAA‐qPCR) assay

    • Wed, 06/22/2022 - 04:00
    • International Journal of Dairy Technology
    • International Journal of Dairy Technology, EarlyView.

      • Bacterial pathogens
      • Cronobacter
  12. Complete genome sequences and genomic characterization of five plasmids harbored by environmentally persistent Cronobacter sakazakii strains ST83 H322 and ST64 GK1025B obtained from powdered infant formula manufacturing facilities

    • Wed, 06/08/2022 - 04:00
    • Gut Pathogens
    • Background Cronobacter sakazakii is a foodborne pathogen that causes septicemia, meningitis, and necrotizing enterocolitis in neonates and infants. The current research details the full genome sequences of two extremely persistent C. sakazakii strains (H322 and GK1025B) isolated from powdered infant formula (PIF) manufacturing settings.

      • Bacterial pathogens
      • Cronobacter
  13. Protective effect of bovine lactoferrin against Cronobacter sakazakii in human intestinal Caco-2/TC7 cells

    • Wed, 06/08/2022 - 04:00
    • International Dairy Journal
    • Milk is a source of bioactive proteins with defensive properties, of great value for protecting the newborn. The activity of bovine milk lactoferrin (LF) was investigated as an antibacterial agent in the internalisation of the emergent pathogen Cronobacter sakazakii into Caco-2/TC7 cells, a model of human intestinal epithelium. The effect of LF on oxidative stress and expression of Toll-like receptors (TLR) was also investigated.

      • Bacterial pathogens
      • Cronobacter
  14. Sandwich capture ultrasensitive sensor based on biohybrid interface for the detection of Cronobacter sakazakii

    • Sat, 06/04/2022 - 04:00
    • Applied Microbiology and Biotechnology
    • A simple, rapid and ultrasensitive visual sensing method for the detection of Cronobacter sakazakii (C. sakazakii) based on a biohybrid interface was established. During the entire sensing process, quadruple-cascade amplification showed its superior sensing performance. First, the prepared immunomagnetic beads (IMB) were used to isolate and enrich specific targets from the food matrix.

      • Bacterial pathogens
      • Cronobacter
  15. A portable toolbox based on time-resolved fluoroimmunoassay and immunomagnetic separation for Cronobacter sakazakii on-site detection in dairy

    • Fri, 06/03/2022 - 04:00
    • International Dairy Journal
    • A portable toolbox based on time-resolved fluoroimmunoassay and immunomagnetic separation was designed for on-site detection of Cronobacter sakazakii in dairy without advanced facilities. In this study, C. sakazakii was recognised and captured in milk by immunomagnetic beads, and europium chelated nanoparticles were used as signal probes in fluoroimmunoassays for quantitative analysis.

      • Bacterial pathogens
      • Cronobacter
  16. Genomic Characterization of Cronobacter spp. and Salmonella spp. Strains Isolated From Powdered Infant Formula in Chile

    • Thu, 06/02/2022 - 04:00
    • Frontiers in Microbiology
    • This study characterized five Cronobacter spp. and six Salmonella spp. strains that had been isolated from 155 samples of powdered infant formula (PIF) sold in Chile and manufactured in Chile and Mexico in 2018–2020. Two strains of Cronobacter sakazakii sequence type (ST) ST1 and ST31 (serotypes O:1 and O:2) and one strain of Cronobacter malonaticus ST60 (O:1) were identified.

      • Bacterial pathogens
      • Cronobacter
      • Salmonella
  17. Virulence and DNA sequence analysis of Cronobacter spp. isolated from infant cereals

    • Sat, 05/28/2022 - 04:00
    • International Journal of Food Microbiology
    • Cronobacter spp. is an opportunistic pathogen that causes severe infections, affecting newborns and infants, and is also an emerging cause of hospital-acquired infection in elderly populations. These infections are mainly associated with the consumption of infant formulas, even though these bacteria have been isolated from other foods as well. Cronobacter spp. invades epithelial cells and escapes the immune response mechanisms, multiplying inside macrophages.

      • Bacterial pathogens
      • Cronobacter
  18. Antibacterial activity of a polyphenol-rich haskap (Lonicera caerulea L.) extract and tannic acid against Cronobacter spp.

    • Wed, 05/25/2022 - 06:00
    • Food Control
    • The antibacterial properties of methanolic haskap (Lonicera caerulea L.) berry extracts and tannic acid were examined against Cronobacter spp. with a view to determine their potential for the control of this human pathogen in powdered infant formula. The total phenolic content (TPC) and composition of methanolic extracts from three different cultivars were determined.

      • Bacterial pathogens
      • Cronobacter
  19. Case report of Curtobacterium isolated from a catheter tip sample misidentified as Cronobacter

    • Tue, 05/24/2022 - 04:00
    • Letters in Applied Microbiology
    • Letters in Applied Microbiology, Accepted Article. The Curtobacterium genus is a member of the family Microbacteriaceae, and Curtobacterium species are recognized as plant pathogens. The aim of this study was to investigate a dubious result of species identification for an infection located on a catheter tip of a patient with Covid-19.

      • Bacterial pathogens
      • Cronobacter
  20. An ultrasensitive biosensor for virulence ompA gene of Cronobacter sakazakii based on boron doped carbon quantum dots-AuNPs nanozyme and exonuclease III-assisted target-recycling strategy

    • Sat, 05/21/2022 - 04:00
    • Food Chemistry
    • This work presented an electrochemical biosensor for the detection of virulence outer membrane protein A (ompA) gene of Cronobacter sakazakii (C. sakazakii), which was based on the mimic peroxidase activity of boron doped quantum dots-Au nanoparticles (BQDs-AuNPs) and a signal amplification strategy of exonuclease III (Exo III)-assisted target-recycling (EATR). The electrochemical signal was come from the electrochemical reduction of H2O2 by BQDs-AuNPs nanozyme.

      • Bacterial pathogens
      • Cronobacter
  21. Evaluation of Potential for Butyl and Heptyl Para-Hydroxybenzoate Enhancement of Thermal Inactivation of Cronobacter sakazakii during Rehydration of Powdered Infant Powdered Infant Formula and Non-Fat Dry Milk.

    • Fri, 05/20/2022 - 04:00
    • Prior studies have demonstrated that parabens enhanced the thermal inactivation of foodborne pathogens including Cronobacter sakazakii , Salmonella enterica serotype Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in model systems. However, there have been few studies looking at this phenomenon in actual food systems. The current study evaluated the potential enhancement of thermal inactivation of C.

      • Bacterial pathogens
      • Cronobacter
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
      • Listeria monocytogenes
      • Salmonella
  22. Proteomic Analysis Revealed Metabolic Inhibition and Elongation Factor Tu Deamidation by p-Coumaric Acid in Cronobacter sakazakii

    • Mon, 05/09/2022 - 04:00
    • Frontiers in Microbiology
    • Screening drugs and compounds to fight against Cronobacter sakazakii (C. sakazakii), one of the most common pathogens that can cause fatal necrotizing enterocolitis, septicema and meningitis, is still needed. We found that p-coumaric acid (pCA) has an inhibitory effect on C. sakazakii in vitro and in vivo. Proteomic changes of C. sakazakii BAA-894 exposed to pCA were studied to reveal the antibacterial mechanisms involved. A total of 1,553 proteins were identified in C.

      • Bacterial pathogens
      • Cronobacter
  23. Antimicrobial activity and mechanism of isothiocyanate from Moringa oleifera seeds against Bacillus cereus and Cronobacter sakazakii and its application in goat milk

    • Wed, 05/04/2022 - 05:00
    • Food Control
    • This study was conducted to investigate the antibacterial activity and mechanism of (4-[(4′-O-acetyl-α-L-rhamnosyloxy)benzyl] isothiocyanate) (RBITC) from Moringa oleifera seeds at the molecular level against Bacillus cereus (B. cereus) and Cronobacter sakazakii (C. sakazakii) and to further analyze the application of RBITC in goat milk.

      • Bacterial pathogens
      • Bacillus cereus
      • Cronobacter
  24. A sensitive visual DNAzyme-based strategy for Cronobacter sakazakii in PIF by aRPA

    • Sun, 05/01/2022 - 04:00
    • Food Control
    • Cronobacter sakazakii, as a most important foodborne pathogen in powdered infant formula (PIF), can cause diseases with high mortality in infants and young children, and has become a key monitoring target in dairy industry. In this study, a visual detection strategy based on DNAzyme and asymmetry recombinase polymerase amplification (aRPA) was developed for monitoring C. sakazakii in PIF.

      • Bacterial pathogens
      • Cronobacter
  25. Occurrence, Molecular Characterization, and Antibiotic Resistance of Cronobacter sakazakii in Goat Milk-Based Infant Formula from Shaanxi Province, China

    • Fri, 04/22/2022 - 04:00
    • Foodborne Pathogens and Disease
    • This study aimed to investigate the prevalence of Cronobacter sakazakii in goat milk-based infant formula (GIF) collected from Shaanxi Province, China, and reveal the molecular characterization and antibiotic resistance profile of these isolates. A total of 750 GIF samples were collected from the retail markets in 5 cities in Shaanxi Province from February 2019 to February 2021. Molecular characterization was investigated using multilocus sequence typing and O-antigen serotyping.

      • Bacterial pathogens
      • Cronobacter