Research Publications (Food Safety)

Publication date: 16 March 2016
, Volume 221

Author(s): Catherine M. Burgess, Andrea Gianotti, Nadia Gruzdev, John Holah, Susanne Knøchel, Angelika Lehner, Edyta Margas, Stephan Schmitz Esser, Shlomo Sela (Saldinger), Odile Tresse

Publication date: Available online 21 January 2016


Author(s): Meiling Jiang, Fen Zhang, Cuixiang Wan, Yonghua Xiong, Nagendra P. Shah, Hua Wei, Xueying Tao

Publication date: May 2016
, Volume 63

Author(s): Jielin Yang, Yuping He, Jing Jiang, Wanyi Chen, Qin Gao, Liangwen Pan, Chunlei Shi

Cronobacter sakazakii is an important pathogen that causes high mortality in infants. Due to its occasional antibiotic resistance, a bacteriophage approach might be an alternative effective method for the control of this pathogen. To develop a novel biocontrol agent using bacteriophages, the C. sakazakii-infecting phage CR5 was newly isolated and characterized. Interestingly, this phage exhibited efficient and relatively durable host lysis activity.

Abstract

Abstract

Aims

The objective of this study was to determine the combined effect of thymoquinone (TQ) and mild heat on C. sakazakii in reconstituted infant formula.

Publication date: 23 December 2015
, Volume 215
Author(s): Lorraine Endersen, Aidan Coffey, R. Paul Ross, Olivia McAuliffe, Colin Hill, Jim O'Mahony

Kim, Dong-Hyeon et al.

Publication date: 2 December 2015
, Volume 214
Author(s): H. Margot, M.H. Zwietering, H. Joosten, Emer O'Mahony, R. Stephan

Publication date: Available online 5 August 2015

Author(s): Kan Deng, Tingtao Chen, Qinglong Wu, Hongbo Xin, Qiang Wei, Ping Hu, Xiaolei Wang, Xin Wang, Hua Wei, Nagendra P. Shah

Cronobacter species are Gram-negative opportunistic pathogens that can cause serious infections in neonates. The lipopolysaccharides (LPSs) that form part of the outer membrane of such bacteria are possibly related to the virulence of particular bacterial strains. However, currently there is no clear overview of O-antigen diversity within the various Cronobacter strains and links with virulence. In this study, we tested a total of 82 strains, covering each of the Cronobacter species.

Cronobacter spp. (formerly defined as Enterobacter sakazakii) are opportunistic bacterial pathogens of both infants and adults. In this study, we analyzed 70 Cronobacter isolates from powdered infant formula (PIF) and an infant formula production facility in China to determine possible contamination routes. The strains were profiled by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), PCR-based O-antigen serotyping, and ompA and rpoB sequence analyses.

Publication date: January 2016
, Volume 59
Author(s): Chao Shi , Yi Sun , Xiaorong Zhang , Zhiwei Zheng , Miaochun Yang , Hao Ben , Kaikuo Song , Yifang Cao , Yifei Chen , Xin Liu , Rui Dong , Xiaodong Xia

Publication date: December 2015
, Volume 52
Author(s): Lan Hu , Christopher J. Grim , Augusto A. Franco , Karen G. Jarvis , Vengopal Sathyamoorthy , Mahendra H. Kothary , Barbara A. McCardell , Ben D. Tall

Publication date: Available online 2 July 2015

Author(s): Renhui Huang , Xueying Tao , Cuixiang Wan , Shengjie Li , Hengyi Xu , Feng Xu , Nagendra P. Shah , Hua Wei

Yan Huang, Yiheng Pang, Hong Wang, Zhengzhu Tang, Yan Zhou, Weiyu Zhang, Xiugui Li, Dongmei Tan, Jian Li, Ying Lin, Xiaoling Liu, Weiyi Huang, Yunliang Shi

Publication date: October 2015
, Volume 51
Author(s): Ana Graça , David Santo , Eduardo Esteves , Carla Nunes , Maribel Abadias , Célia Quintas

Publication date: July 2015
, Volume 98, Issue 7
Author(s): Xin Wang , Qinglong Wu , Kan Deng , Qiang Wei , Ping Hu , Jingjing He , Huan Liu , Yong Zheng , Hua Wei , Nagendra P. Shah , Tingtao Chen

Publication date: Available online 12 June 2015

Author(s): Chen Qiming , Tao Tingting , Bie Xiaomei , Lu Yingjian , Lu Fengxia , Zhai Ligong , Lu Zhaoxin

Jackson, Emily E. et al. In 2010, two infants became ill at a hospital in Mexico. Subsequently, a range of clinical, environmental, and powdered and rehydrated infant formula isolates were identified by using a combination of phenotyping and PCR probes. The strains were clustered according to pulsed-field gel electrophoresis. The causative agent was reported as Cronobacter sakazakii, with powdered infant formula (PIF) identified as the likely source of the infections.

Cronobacter species are opportunistic pathogens commonly found in the environment. Among the seven Cronobacter species, Cronobacter sakazakii sequence type 4 (ST-4) is predominantly associated with recorded cases of infantile meningitis. This study reports on a 26-month powdered infant formula (PIF) surveillance program in four production facilities located in distinct geographic regions.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

Wang, Xiwen et al. Cronobacter sakazakii is an emerging pathogen that causes severe and life-threatening conditions including meningitis, bacteremia, and necrotizing enterocolitis. An animal model study for extrapolation of C. sakazakii infection can provide a better understanding of pathogenesis. However, methods for real-time monitoring of the course of C. sakazakii infection in living animals have been lacking. We developed a bioluminescent C.

The occurrence of outbreaks of Cronobacter sakazakii causing necrotizing meningitis in China highlights the need for strain characterization of this pathogenic species. In this study, SMM system was utilized to mine for new molecular markers of C. sakazakii. One C. sakazakii-specific CDS (>ref|NC_009778.1|:c1077329-1076055) with a length of 1,275 bp was used to design a primer set. A PCR assay was developed and optimized to detect C. sakazakii.

Cronobacter species are foodborne pathogens associated with neonatal meningitis, sepsis and necrotizing enterocolitis through consumption of contaminated powdered milk. However, there are no specific targets used for differentiating Cronobacter species. The DNA-based assay and polyphasic analysis have facilitated the detection and typing of Cronobacter species in foodborne outbreaks. In this study, polymerase chain reaction (PCR) and dot hybridization for detecting Cronobacter spp.