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Research Publications (Food Safety)

The Food Safety Publications tracks research that is published across national and international peer-reviewed journals. Recent articles are available ahead of print and searchable by Journal, Article Title, and Category. The research publications are tracked across six categories: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, Produce Safety, and Viruses. Articles produced by USDA Grant Funding Agencies and FDA Grant Funding Agencies are also tracked.

Displaying 1 - 25 of 326

  1. Apyrase decreases phage induction and Shiga toxin release from E. coli O157:H7 and has a protective effect during infection

    • Sat, 09/24/2022 - 04:00
    • Gut Microbes
      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  2. Acid shock protein Asr induces protein aggregation to promote E. coli O157:H7 entering viable but non-culturable state under high pressure carbon dioxide stress

    • Fri, 09/16/2022 - 04:00
    • Food Microbiology
    • Under stressful conditions, bacteria can enter viable but non-culturable (VBNC) state to survive. VBNC cells lost ability to grow on routine culture medium but are still alive and may revive in suitable conditions. The revived cells can consume nutrients or produce toxins, leading to food spoilage or human illness, posing great risk to food safety and public health.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  3. Prevalence and molecular characterization of shiga toxin-producing Escherichia coli in animal source foods and green leafy vegetables

    • Wed, 09/14/2022 - 04:00
    • Food Science and Technology International
    • Shiga toxin–producing Escherichia coli (STEC) has emerged as important enteric foodborne zoonotic pathogens of considerable public health significance worldwide. The aim of this study was to determine the prevalence of the top seven STEC serotypes and to identify these serotypes in samples of animal source foods and vegetables. A total of 294 samples including 84 meat samples, 135 milk and dairy product samples and 75 green leafy vegetable samples were tested.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
      • Produce Safety
      • Leafy Greens
  4. Exploring the nature of interaction between shiga toxin producing Escherichia coli (STEC) and free-living amoeba - Acanthamoeba sp

    • Sat, 09/10/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • Free-living amoebae (FLA) are widely distributed protozoa in nature, known to cause severe eye infections and central nervous system disorders. There is growing attention to the potential role that these protozoa could act as reservoirs of pathogenic bacteria and, consequently, to the possibility that, the persistence and spread of the latter may be facilitated, by exploiting internalization into amoebae.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  5. Accurate detection of Escherichia coli O157:H7 and Salmonella enterica serovar typhimurium based on the combination of next-generation sequencing and droplet digital PCR

    • Wed, 09/07/2022 - 04:00
    • LWT
    • Shiga-toxin producing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium are important foodborne pathogens which are threatened to human health seriously.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Salmonella
  6. Behaviour of Escherichia coli O157:H7 in raw and mild pasteurised donkey milk treated with high pressure

    • Wed, 09/07/2022 - 04:00
    • International Dairy Journal
    • The survival of E. coli O157:H7 strain (NCTC 12900) was assessed in raw donkey milk, in mild pasteurised donkey milk (50 ° C for 30 min), in donkey milk processed by HP at 400 MPa at 3 °C for 60 s, and in donkey milk treated with a combination of the two technologies. The results showed that E. coli O157:H7 was able to survive in raw donkey milk for 15 days and in mild pasteurised donkey milk for 4 days.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  7. Genomic characterization of a novel bacteriophage STP55 revealed its prominent capacity in disrupting the dual-species biofilm formed by Salmonella Typhimurium and Escherichia coli O157: H7 strains

    • Mon, 09/05/2022 - 04:00
    • Archives of Microbiology
    • Salmonella and Escherichia coli are important foodborne pathogens, forming bacterial biofilms that contribute to their virulence, antimicrobial resistance, and survival on surfaces. Broad lytic phages are promising alternatives to conventional technologies for pathogen biocontrol and reducing biofilms. Herein, we isolated and characterized a novel polyvalent phage STP55 that not only lyse some serotypes of Salmonella, but also some E. coli strains.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Salmonella
  8. Protective role of Acinetobacter and Bacillus for Escherichia coli O157:H7 in biofilms against sodium hypochlorite and extracellular matrix-degrading enzymes

    • Sun, 09/04/2022 - 04:00
    • Food Microbiology
    • Foodborne pathogenic bacteria in multi-species biofilms in food manufacturing facilities have been suspected to be the cause of cross-contamination leading to foodborne illness. We studied if cafeteria kitchen-associated bacterial isolates can have any protective effect on E. coli O157:H7 in biofilm against extracellular polymeric substances (EPS)-degrading enzymes and sodium hypochlorite.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  9. trans‐Cinnamaldehyde‐encapsulated zeolitic imidazolate framework‐8 nanoparticle complex solutions to inactivate Escherichia coli O157:H7 on fresh spinach leaves

    • Thu, 09/01/2022 - 04:00
    • Journal of Food Science
    • Journal of Food Science, EarlyView. This study synthesized and characterized ZIF-8 nanoparticles encapsulated with trans-cinnamaldehyde oil (TC) and evaluated their antimicrobial effectiveness against Escherichia coli O157:H7 on fresh spinach leaves. The antimicrobial activity of different mass ratios of TC-encapsulated ZIF-8 against E. coli O157:H7 (ATCC 43895) strain was assessed and the best mass ratio of 1:2 TC to ZIF-8 identified.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  10. Unraveling the mechanism of the synergistic antimicrobial effect of cineole and carvacrol on Escherichia coli O157:H7 inhibition and its application on fresh-cut cucumbers

    • Thu, 09/01/2022 - 04:00
    • Food Control
    • The objective of this study was to evaluate the mechanism of the synergistic antimicrobial effect of cineole (CN) and carvacrol (CR) on E. coli and its application on fresh-cut cucumbers. Herein, the CN/CR synergy could effectively inhibit E. coli by destroying its bacterial cell structure and causing the leakage of intracellular organic matters. For the first time, CN/CR synergy interaction on E.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  11. Genomic Analysis of Shiga Toxin-Producing E. coli O157 Cattle and Clinical Isolates from Alberta, Canada

    • Thu, 09/01/2022 - 04:00
    • Toxins
    • Shiga toxin (stx) is the principal virulence factor of the foodborne pathogen, Shiga toxin-producing Escherichia coli (STEC) O157:H7 and is associated with various lambdoid bacterio (phages). A comparative genomic analysis was performed on STEC O157 isolates from cattle (n = 125) and clinical (n = 127) samples to characterize virulence genes, stx-phage insertion sites and antimicrobial resistance genes that may segregate strains circulating in the same geographic region.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  12. Research Paper Sunlight Parameters Influence the Survival and Decline of Salmonella and Escherichia coli in Water

    • Wed, 08/31/2022 - 04:00
    • The effect of variations in temperature, ultraviolet (UV) radiation, and sunlight intensity on generic Escherichia coli , E. coli O157:H7, Salmonella Newport and antibiotic resistant (ABR) variants of E. coli O157:H7 and S . Newport exposed to sunlight was evaluated. Bacterial strains suspended in sterile deionized water at a concentration of 8 log CFU/ml were exposed to sunlight on three different days for 180 min; control treatments were stored in the dark.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
      • Salmonella
  13. Synergistic bactericidal effect of nisin and phytic acid against Escherichia coli O157:H7

    • Sun, 08/28/2022 - 04:00
    • Food Control
    • The food industry must prevent food contamination caused by pathogenic Escherichia coli strains as they pose a severe public health threat worldwide and lead to unimaginable economic losses. In this study, the synergistic antibacterial activity in vitro of nisin and phytic acid, a natural metal chelate, against 5 foodborne isolates of E. coli were evaluated by a checkerboard assay.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  14. TiO2-based photocatalyst Generated Reactive Oxygen Species cause cell membrane disruption of Staphylococcus aureus and Escherichia coli O157:H7

    • Tue, 08/23/2022 - 04:00
    • Food Microbiology
    • Photocatalysts, including titanium dioxide (TiO2), have attracted much attention in food safety for controlling foodborne pathogens. However, the study of the photocatalytic activity on various food-surrounding media and the factors that affect the efficacy of photocatalytic inactivation is incomplete. In this study, to inactivate foodborne pathogens in food-surrounding environments, TiO2-based photocatalysts with ultraviolet A (UVA, 365 nm) and visible light (VIS, 405 nm) were employed.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Staphylococcus aureus
  15. Plant species‐dependent transmission of Escherichia coli O157:H7 from the spermosphere to cotyledons and first leaves

    • Tue, 08/16/2022 - 04:00
    • Environmental Microbiology Reports
    • Environmental Microbiology Reports, EarlyView. The colonization of six edible plant species: alfalfa, broccoli, coriander, lettuce, parsley and rocket, by the human pathogen Shigatoxigenic Escherichia coli was investigated following two modes of artificial inoculation of seeds, by soaking or watering.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  16. Sporadic Occurrence of Enteroaggregative Shiga Toxin–Producing Escherichia coli O104:H4 Similar to 2011 Outbreak Strain

    • Fri, 08/12/2022 - 04:00
    • Emerging Infectious Diseases
    • We describe the recent detection of 3 Shiga toxin–producing enteroaggregative Escherichia coli O104:H4 isolates from patients and 1 from pork in the Netherlands that were genetically highly similar to isolates from the 2011 large-scale outbreak in Europe. Our findings stress the importance of safeguarding food supply production chains to prevent future outbreaks.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  17. Intestinal mucus-derived metabolites modulate virulence of a clade 8 enterohemorrhagic Escherichia coli O157:H7

    • Mon, 08/08/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • The human colonic mucus is mainly composed of mucins, which are highly glycosylated proteins. The normal commensal colonic microbiota has mucolytic activity and is capable of releasing the monosaccharides contained in mucins, which can then be used as carbon sources by pathogens such as Enterohemorrhagic Escherichia coli (EHEC).

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  18. Tracking the Transmission of Antimicrobial-Resistant Non-O157 Escherichia coli and Salmonella Isolates at the Interface of Food Animals and Fresh Produce from Agriculture Operations Using Whole-Genome Sequencing

    • Fri, 08/05/2022 - 04:00
    • Foodborne Pathogens and Disease
    • An increasing number of outbreaks are caused by foodborne pathogens such as Escherichia coli and Salmonella, which often harbor antimicrobial resistance (AMR) genes. We previously demonstrated the transmission of pathogens from animal operations to produce fields on sustainable farms, which illustrated an urgent need to develop and implement novel prevention methods and remediation practices such as the vegetative buffer zone (VBZ) to prevent this movement.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Salmonella
  19. Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices

    • Fri, 08/05/2022 - 04:00
    • Frontiers in Microbiology
    • Enterohemorrhagic Escherichia coli (EHEC) O157:H7 and Enterotoxigenic E. coli (ETEC) are important foodborne pathogens, causing serious food poisoning outbreaks worldwide. Bacteriophages, as novel antibacterial agents, have been increasingly exploited to control foodborne pathogens.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  20. Molecular characterization and phylogeny of Shiga toxin-producing Escherichia coli derived from cattle farm

    • Thu, 08/04/2022 - 04:00
    • Frontiers in Microbiology
    • Shiga toxin-producing Escherichia coli (STEC) is an important food-borne pathogen, which can cause diseases such as diarrhea, hemorrhagic enteritis, and hemolytic uremic syndrome in humans. Twelve STEC isolates were collected from beeves and feces of commercial animals in China between 2019 and 2020 for this study.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  21. Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from the lacZ Gene Restores the Lactose Utilization Defect of Shiga Toxin-Producing Escherichia coli O121:H19 Strains and Is Responsible for Their Delayed Lactose Utilization Phenotype

    • Tue, 08/02/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Lactose utilization is one of the general biochemical characteristics of Escherichia coli, and the lac operon is responsible for this phenotype, which can be detected on lactose-containing media, such as MacConkey agar, after 24 h of incubation. However, some Shiga toxin-producing E.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  22. L. monocytogens exhibited less cell membrane damage, lipid peroxidation, and intracellular reactive oxygen species accumulation after plasma-activated water treatment compared to E. coli O157:H7 and S. Typhimurium

    • Mon, 08/01/2022 - 04:00
    • Food Microbiology
    • This study investigated the bactericidal activity of plasma-activated water (PAW) generated with a remote discharge reactor against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  23. Lymphostatin, a virulence factor of attaching and effacing Escherichia coli, inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis

    • Sat, 07/30/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • Lymphostatin is a virulence factor of enteropathogenic E. coli (EPEC) and non-O157 serogroup enterohaemorrhagic E. coli. Previous studies using whole-cell lysates of EPEC showed that lymphostatin inhibits the mitogen-activated proliferation of bulk human peripheral blood mononuclear cells (PBMCs) and the production of cytokines IL-2, IL-4, IL-5, and IFN-γ.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  24. Effects of temperature, pH, and sodium chloride on antimicrobial activity of magnesium oxide nanoparticles against Escherichia coli O157:H7

    • Sat, 07/30/2022 - 04:00
    • Journal of Applied Microbiology
    • Journal of Applied Microbiology, Accepted Article. Aim This study was done to determine the effects of temperature, pH, and sodium chloride (NaCl) on antimicrobial activity of magnesium oxide (MgO) nanoparticles (NPs) against Escherichia coli O157:H7. Methods and Results Culture conditions were established by varying the pH (5.0, 7.2, and 9.0), NaCl concentration (0.5, 2.0, 3.5, and 5.0%, w/v), and incubation temperatures (4, 12, 22, and 37°C).

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  25. Multidrug-Resistant Enteropathogenic Escherichia coli Isolated from Diarrhoeic Calves, Milk, and Workers in Dairy Farms: A Potential Public Health Risk

    • Tue, 07/26/2022 - 04:00
    • Antibiotics
    • Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhoeagenic diseases in humans and cattle worldwide. The emergence of multidrug-resistant (MDR) EPEC from cattle sources is a public health concern. A total of 240 samples (75 diarrhoeic calves, 150 milk samples, and 15 workers) were examined for prevalence of EPEC in three dairy farms in Egypt.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli