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Research Publications (Food Safety)

The Food Safety Publications tracks research that is published across national and international peer-reviewed journals. Recent articles are available ahead of print and searchable by Journal, Article Title, and Category. The research publications are tracked across six categories: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, Produce Safety, and Viruses. Articles produced by USDA Grant Funding Agencies and FDA Grant Funding Agencies are also tracked.

Displaying 1 - 25 of 893

  1. Longitudinal Changes in Campylobacter and the Litter Microbiome throughout the Broiler Production Cycle

    • Wed, 08/10/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print.

      • Bacterial pathogens
      • Campylobacter
  2. Efficacy of Acidified Oils against Salmonella in Low-Moisture Environments

    • Tue, 08/09/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print.

      • Bacterial pathogens
      • Salmonella
  3. Genomic Analysis Reveals Adaptation of Vibrio campbellii to the Hadal Ocean

    • Wed, 08/03/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. The genus Vibrio is characterized by high metabolic flexibility and genome plasticity and is widely distributed in the ocean from euphotic layers to deep-sea environments. The relationship between genome features and environmental adaptation strategies of Vibrio has been extensively investigated in coastal environments, yet very little is known about their survival strategies in oligotrophic deep-sea.

      • Bacterial pathogens
      • Vibrio
  4. Time-Dependent Biosensor Fluorescence as a Measure of Bacterial Arsenic Uptake Kinetics and Its Inhibition by Dissolved Organic Matter

    • Tue, 08/02/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Microbe-mediated transformations of arsenic (As) often require As to be taken up into cells prior to enzymatic reaction. Despite the importance of these microbial reactions for As speciation and toxicity, understanding of how As bioavailability and uptake are regulated by aspects of extracellular water chemistry, notably dissolved organic matter (DOM), remains limited.

      • Chemical contaminants
      • Heavy Metals
  5. Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from the lacZ Gene Restores the Lactose Utilization Defect of Shiga Toxin-Producing Escherichia coli O121:H19 Strains and Is Responsible for Their Delayed Lactose Utilization Phenotype

    • Tue, 08/02/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Lactose utilization is one of the general biochemical characteristics of Escherichia coli, and the lac operon is responsible for this phenotype, which can be detected on lactose-containing media, such as MacConkey agar, after 24 h of incubation. However, some Shiga toxin-producing E.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  6. Characterization of Functional B-Cell Epitopes at the Amino Terminus of Shigella Invasion Plasmid Antigen B (IpaB)

    • Thu, 07/21/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Shigella invasion plasmid antigen B (IpaB) plays an important role in causing shigellosis. While IpaB’s protein structure, contribution to disease mechanism, and protective immunity against Shigella infection have been well studied, the significance of individual antigenic domains, especially at the N terminus, has not been systematically characterized.

      • Bacterial pathogens
      • Shigella
  7. Dysregulation of Cell Envelope Homeostasis in Staphylococcus aureus Exposed to Solvated Lignin

    • Wed, 07/20/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Lignin is an aromatic plant cell wall polymer that facilitates water transport through the vasculature of plants and is generated in large quantities as an inexpensive by-product of pulp and paper manufacturing and biorefineries. Although lignin’s ability to reduce bacterial growth has been reported previously, its hydrophobicity complicates the ability to examine its biological effects on living cells in aqueous growth media.

      • Bacterial pathogens
      • Staphylococcus aureus
  8. Subtyping Evaluation of Salmonella Enteritidis Using Single Nucleotide Polymorphism and Core Genome Multilocus Sequence Typing with Nanopore Reads

    • Thu, 07/14/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Whole-genome sequencing (WGS) for public health surveillance and epidemiological investigation of foodborne pathogens predominantly relies on sequencing platforms that generate short reads.

      • Bacterial pathogens
      • Salmonella
  9. Investigating the Relationship between Nitrate, Total Dissolved Nitrogen, and Phosphate with Abundance of Pathogenic Vibrios and Harmful Algal Blooms in Rehoboth Bay, Delaware

    • Thu, 07/07/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Vibrio spp. and phytoplankton are naturally abundant in marine environments. Recent studies have suggested that the co-occurrence of phytoplankton and the pathogenic bacterium Vibrio parahaemolyticus is due to shared ecological factors, such as nutrient requirements.

      • Bacterial pathogens
      • Vibrio
  10. Regulation of Enterotoxins Associated with Bacillus cereus Sensu Lato Toxicoinfection

    • Thu, 06/23/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Bacillus cereus sensu lato (s.l.) includes foodborne pathogens, as well as beneficial microorganisms, such as bioinsecticides. Some of the beneficial and commercially used B. cereus s.l. strains have been shown to carry enterotoxin genes, the products of which can cause toxicoinfection in humans.

      • Bacterial pathogens
      • Bacillus cereus
  11. Inhibition of Sulfate Reduction and Cell Division by Desulfovibrio desulfuricans Coated in Palladium Metal

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. The growth of sulfate-reducing bacteria (SRB) and associated hydrogen sulfide production can be problematic in a range of industries such that inhibition strategies are needed. A range of SRB can reduce metal ions, a strategy that has been utilized for bioremediation, metal recovery, and synthesis of precious metal catalysts.

      • Bacterial pathogens
      • Vibrio
  12. In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. The alternative sigma factor B (σB) contributes to the stress tolerance of the foodborne pathogen Listeria monocytogenes by upregulating the general stress response. We previously showed that σB loss-of-function mutations arise frequently in strains of L. monocytogenes and suggested that mild stresses might favor the selection of such mutations. In this study, we performed in vitro evolution experiments (IVEE) where L.

      • Bacterial pathogens
      • Listeria monocytogenes
  13. Assessment of Listeria monocytogenes Surface Proteins Identified from Proteomics Analysis for Use as Diagnostic Biomarkers

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. The Gram-positive bacterium Listeria monocytogenes is an important pathogen that causes a foodborne illness with a high percentage of fatalities. Surface proteins, specifically expressed from a wide range of L. monocytogenes serotypes under selective enrichment culture conditions, can serve as targets for the detection and isolation of this pathogen using antibody-based methods.

      • Bacterial pathogens
      • Listeria monocytogenes
  14. A Novel, NADH-Dependent Acrylate Reductase in Vibrio harveyi

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Bacteria coping with oxygen deficiency use alternative terminal electron acceptors for NADH regeneration, particularly fumarate. Fumarate is reduced by the FAD_binding_2 domain of cytoplasmic fumarate reductase in many bacteria. The variability of the primary structure of this domain in homologous proteins suggests the existence of reducing activities with different specificities.

      • Bacterial pathogens
      • Vibrio
  15. Nonsynonymous Mutations in fepR Are Associated with Adaptation of Listeria monocytogenes and Other Listeria spp. to Low Concentrations of Benzalkonium Chloride but Do Not Increase Survival of L. monocytogenes and Other Listeria spp. after Exposure to Benz

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Selection for Listeria monocytogenes strains that are tolerant to quaternary ammonium compounds (such as benzalkonium chloride [BC]) is a concern across the food industry, including in fresh produce processing environments. This study evaluated the ability of 67 strains of produce-associated L. monocytogenes and other Listeria spp.

      • Bacterial pathogens
      • Listeria monocytogenes
  16. Viral Proteins Involved in the Adsorption Process of Deep-Purple, a Siphovirus Infecting Members of the Bacillus cereus Group

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. The infection of a bacterium by a tailed phage starts from the adsorption process, which consists of a specific and strong interaction between viral proteins called receptor binding proteins (RBPs) and receptors located on the bacterial surface.

      • Bacterial pathogens
      • Bacillus cereus
  17. Analysis of Salmonella enterica Isolated from a Mixed-Use Watershed in Georgia, USA: Antimicrobial Resistance, Serotype Diversity, and Genetic Relatedness to Human Isolates

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. As the cases of Salmonella enterica infections associated with contaminated water are increasing, this study was conducted to address the role of surface water as a reservoir of S. enterica serotypes. We sampled rivers and streams (n = 688) over a 3-year period (2015 to 2017) in a mixed-use watershed in Georgia, USA, and 70.2% of the total stream samples tested positive for Salmonella.

      • Bacterial pathogens
      • Salmonella
  18. The Use of Disinfectant in Barn Cleaning Alters Microbial Composition and Increases Carriage of Campylobacter jejuni in Broiler Chickens

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. To maintain food safety and flock health in broiler chicken production, biosecurity approaches to keep chicken barns free of pathogens are important. Canadian broiler chicken producers must deep clean their barns with chemical disinfectants at least once annually (full disinfection [FD]) and may wash with water (water wash [WW]) throughout the year.

      • Bacterial pathogens
      • Campylobacter
  19. Subinhibitory Cefotaxime and Levofloxacin Concentrations Contribute to Selection of Pseudomonas aeruginosa in Coculture with Staphylococcus aureus

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Bacterial species in the polymicrobial community evolve interspecific interaction relationships to adapt to the survival stresses imposed by neighbors or environmental cues. Pseudomonas aeruginosa and Staphylococcus aureus are two common bacterial pathogens frequently coisolated from patients with burns and respiratory disease.

      • Bacterial pathogens
      • Staphylococcus aureus
  20. Growing Degree-Day Measurement of Cyst Germination Rates in the Toxic Dinoflagellate Alexandrium catenella

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Blooms of many dinoflagellates, including several harmful algal bloom (HAB) species, are seeded and revived through the germination of benthic resting cysts. Temperature is a key determinant of cysts’ germination rate, and temperature–germination rate relationships are therefore fundamental to understanding species’ germling cell production, cyst bed persistence, and resilience to climate warming.

      • Natural toxins
      • Shellfish toxins
  21. Phylogenetic and Phenotypic Analyses of a Collection of Food and Clinical Listeria monocytogenes Isolates Reveal Loss of Function of Sigma B from Several Clonal Complexes

    • Sat, 06/04/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Volume 88, Issue 10, May 2022. To understand the molecular mechanisms that contribute to the stress responses of the important foodborne pathogen Listeria monocytogenes, we collected 139 strains (meat, n = 25; dairy, n = 10; vegetable, n = 8; seafood, n = 14; mixed food, n = 4; and food processing environments, n = 78), mostly isolated in Ireland, and subjected them to whole-genome sequencing.

      • Bacterial pathogens
      • Listeria monocytogenes
  22. Effects of Peroxyacetic Acid Spray and Storage Temperature on the Microbiota and Sensory Properties of Vacuum-Packed Subprimal Cuts of Meat

    • Wed, 05/12/2021 - 10:02
    • Applied and Environmental Microbiology
    • We investigated the impact of peroxyacetic acid (PAA; 200 ppm) spray on the microbiota and shelf life of commercial, vacuum-packed beef stored at chiller temperatures. Ribeye cuts (n = 147) were collected from a local beef plant on the day of production for two consecutive days, with one set collected at the start of work with the PAA spray nozzles turned off (control) and during routine production with the PAA spray nozzles turned on (PAA) each day.

      • Bacterial pathogens
  23. Transmission Dynamics of Shiga Toxin-Producing Escherichia coli in New Zealand Cattle from Farm to Slaughter

    • Wed, 05/12/2021 - 10:02
    • Applied and Environmental Microbiology
    • Cattle are asymptomatic carriers of Shiga toxin-producing Escherichia coli (STEC) strains that can cause serious illness or death in humans. In New Zealand, contact with cattle feces and living near cattle populations are known risk factors for human STEC infection. Contamination of fresh meat with STEC strains also leads to the potential for rejection of consignments by importing countries.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  24. Separate Upper Pathway Ring Cleavage Dioxygenases Are Required for Growth of Sphingomonas wittichii Strain RW1 on Dibenzofuran and Dibenzo-p-Dioxin

    • Wed, 05/12/2021 - 10:02
    • Applied and Environmental Microbiology
    • Sphingomonas wittichii RW1 is one of a few strains known to grow on the related compounds dibenzofuran (DBF) and dibenzo-p-dioxin (DXN) as the sole source of carbon. Previous work by others (B. Happe, L. D. Eltis, H. Poth, R. Hedderich, and K. N.

      • Dioxins
      • Chemical contaminants
  25. Stable Recombinant-Gene Expression from a Ligilactobacillus Live Bacterial Vector via Chromosomal Integration

    • Wed, 05/12/2021 - 10:02
    • Applied and Environmental Microbiology
    • Disease control in animal production systems requires constant vigilance. Historically, the application of in-feed antibiotics to control bacteria and improve performance has been a much-used approach to maintain animal health and welfare. However, the widespread use of in-feed antibiotics is thought to increase the risk of antibiotic resistance developing. Alternative methods to control disease and maintain productivity need to be developed.

      • Bacterial pathogens
      • Clostridium perfringens