Research Publications (Food Safety)

This study investigated the effects of the multi-probiotics consisting of Bacillus subtilis and Clostridium butyricum with varying doses (0%, 0.05%, 0.1%, and 0.2%) on the growth performance, nutrient digestibility, meat quality, and cecal microbes of male broiler chickens. Seven hundred and twenty Ross 308, 1-day-old male broiler chicks were distributed into four dietary groups.

The effect of select antibiotics on Salmonella Typhimurium DT104 persistence in a porcine cecal continuous flow culture was examined under two different washout rates. Porcine continuous flow cultures were conducted in the presence or absence of gram-positive antibiotic carbadox. Carbadox eliminated chemostat anaerobes culturable on Brucella agar under 24 h turnover conditions, allowing Salmonella Typhimurium to persist for 15 days.

In this study, 206 breast fillets were purchased from grocery stores in the province of Quebec and evaluated for the presence of different quality defects. Of these fillets, 48.5% showed breast muscle myopathies (BMM), 19.4% showed pale, soft, and exudative (PSE), and 6.8% showed dark, firm, and dry (DFD) attributes.

This study aimed to evaluate the effect of flavonoid supplementation to a low-protein diet (LCP) on growth performance, nutrient digestibility, excreta bacterial count, and excreta gas emission of broilers. In total, 800 one-day-old Ross 308 broilers (BW; 42.90 ± 1.43 g) were randomly sorted into one of the 4 dietary treatments (10 pens/treatment; 20 birds/pen).

This experiment was accompanied to determine the use of yeast and garlic extract mixture in broiler diets on growth performance, nutrient absorption, excreta microbiota, blood profiles, and meat quality. A total of 792 male Ross 308 broilers (1-day-old, body weight 41 ± 0.5 g, and 5 weeks trial) were randomly allocated. Birds were arbitrarily assigned to one of four nutritive treatments (11 replicates; 18 birds/replicate).

This study aimed to evaluate lytic bacteriophage supplementation in pre-weaned dairy calves on disease occurrence, performance, and biochemical parameters. Two hundred Holstein × Gyr crossbred female calves were divided into two groups: CON, no supplementation; and PHAGE, bacteriophage supplementation (1 g·d−1) from day 3 until day 70 of life. Calves were monitored daily for age of first diarrheal episode and its duration.

Spoiled silages can harbor pathogenic and antimicrobial-resistant microbes. The potential of some antimicrobial additives to inhibit certain pathogenic and antimicrobial-resistant bacteria in air-exposed silage was measured using pure and mixed bacterial cultures. With pure cultures, laurate and monolaurin (5 mg·mL−1) caused decreases (P < 0.05) of 4 to >7 log10 colony-forming units (CFU)·mL−1 in Listeria monocytogenes and Enterococcus faecalis compared to controls.

Chicken Farmers of Canada has been progressively phasing out prophylactic use of antibiotics in broiler chicken production. Consequently, hatcheries, veterinarians, and nutritionists have been mandated to contend with less reliance on the use of preventive antibiotics. A topical concern is the increased risk of proliferation of enteric pathogens leading to poor performance, increased mortality, and compromised welfare.

Spoiled silages can harbor pathogenic and antimicrobial-resistant microbes. The potential of some antimicrobial additives to inhibit certain pathogenic and antimicrobial-resistant bacteria in air-exposed silage was measured using pure and mixed bacterial cultures. With pure cultures, laurate and monolaurin (5 mg·mL−1) caused decreases (P < 0.05) of 4 to >7 log10 colony-forming units (CFU)·mL−1 in Listeria monocytogenes and Enterococcus faecalis compared to controls.

Chicken Farmers of Canada has been progressively phasing out prophylactic use of antibiotics in broiler chicken production. Consequently, hatcheries, veterinarians, and nutritionists have been mandated to contend with less reliance on the use of preventive antibiotics. A topical concern is the increased risk of proliferation of enteric pathogens leading to poor performance, increased mortality, and compromised welfare.

This study evaluated the efficacy of epidermal growth factor (EGF) in piglets challenged with enterotoxigenic Escherichia coli K88 (ETEC). A total of 28 piglets were assigned to the following dietary treatments for 14 d: negative control (NC) (basal diet containing supernatant without EGF), PC (NC + 2.5 g antibiotic·kg−1 feed), EGF120 [basal diet + supernatant with 120 μg EGF·kg−1 body weight (BW)·d−1], and EGF180 (basal diet + supernatant with 180 μg EGF·kg−1 BW·d−1).

An experiment was carried out to investigate the effect of Bacillus subtilis RX7 and B2A supplementation on egg production, egg quality, blood profile, and excreta Salmonella population in laying hens. A total of 192 Hy-Line Brown laying hens (40-wk-old) were randomly assigned into four dietary treatments, each of which was replicated eight times with six hens per replicate in a 5 wk trial.

The objective of this study was (i) to analyze antibiotic residues, two which were beta-lactam antibiotics, one tetracycline, and one quinolone in the milk of lactating animals; (ii) to evaluate the interference of the drug ceftiofur which is considered as discard-zero. The SNAPduo™ ST Plus kit was used to evaluate the presence of beta-lactam antibiotics and tetracyclines in natural milk. Medications based on penicillin, ceftiofur, enrofloxacin, and oxytetracycline were used.

Decades of selection for increased litter size has caused a proportion of sows to consistently produce low birth weight (LBW) litters resulting in economic loss for producers due to reduced piglet survivability and growth. We hypothesized that piglets from LBW litters would have altered gut microbial composition, intestinal architecture, and intestinal transcriptomic profiles compared with piglets from high birth weight (HBW) litters.

The objective of this study was to assess the effects of low-dose zinc oxide (ZnO) supplemented with or without probiotic complex compared with pharmacological ZnO (3000 mg kg−1) on the performance, digestibility, blood metabolites, fecal Lactobacillus and enterobacteria counts of weaned piglets. One-hundred and twenty crossbred piglets were randomly allocated to three treatments based on their initial body weight (BW).