Research Publications (Food Safety)

An efficient in vitro protocol was introduced for the conservation of Nepeta asterotricha, a vulnerable and endangered medicinal species found in the central of Iran for the first time. Growth, phytochemical, and biological traits of in vitro regenerated plant (RP) and acclimated plant (AP) were compared to the mother plant (MP). In addition, the genetic stability of AP was assessed by using inter-simple sequence repeats (ISSR) markers.

The present study is based on the application of H2S as an exogenous antidote in Spinacia oleracea (spinach) plants grown in Cd-contaminated (50 ppm) soil. The different doses of H2S in the form of NaHS (10, 50, 100, 200, and 500 μM) have been applied as a foliar spray to regulate the physiological attributes under Cd toxicity.

A major obstacle to agricultural production and yield quality is heavy metal contamination of the soil and water, which leads to lower productivity and quality of crops. The situation has significantly worsened as a result of the growing population and subsequent rise in food consumption. The growth of nutrient-rich plants is hampered by lead (Pb) toxicity in the soil. Brassica oleracea L.

Chalcone synthase (CHS) is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects. In this study, we used the leaves of D. gotadhora to clone CHS gene (DbCHS). The 1188-bp open reading frame (ORF) was part of the 1373-bp full-length DbCHS clone.

In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extract have been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays.

Cadmium (Cd) toxicity in leaves decreases their photosynthetic efficiency by degrading photosynthetic pigments, reducing the activity of gas exchange parameters and photosystem II (PSII), and producing reactive oxygen species. Although acetone O-(4-chlorophenylsulfonyl) oxime (AO) alleviates stress due to heavy metals in plants, its effects on the photosynthetic apparatus and redox balance under Cd stress are not clear.

Nitric oxide (NO) is known to induce plant resistance for several environmental stresses. The protective roles of NO in cadmium (Cd) toxicity have been well documented for various plant species; nevertheless, little information is available about its molecular regulation in improving Cd tolerance of barley plants.

The main aim of the current study was to investigate the role of Cistus salviifolius leaves extract (CSE) in alleviating the toxic effect of cadmium (Cd) in sorghum (Sorghum bicolor) plants. The plants exposed to Cd (200 µM) exhibited limited growth, reduced biomass, and chlorophyll content compared to unstressed ones. Nevertheless, supplementation of CSE restored the negative effect of Cd and increased biomass and pigment content.

The arsenic (As) contamination demands its remediation from the environment which is naturally possible by the application of Pteris vittata L. However, biomonitoring of phytoremediation potential of P. vittata at chromosomal and DNA level is still meager. The present study was designed to biomonitor the phytoremediation efficiency of P. vittata through phytotoxic and cyto-genotoxic biomarkers assessment using Trigonella foenum-graecum L.

The present study reports the effect of different seasons on polyphenol content and antioxidant potential of ethanolic, methanolic, ethyl acetate, and aqueous extracts of leaves, stems, and roots of Premna integrifolia. Ethyl acetate extract of leaves (EAEPI) collected in the rainy season showed potent antioxidant activity with highest total phenol (74.33 ± 2.26 µg/mg, gallic acid equivalent), and flavonoid (98.83 ± 0.26 µg/mg, rutin equivalent) content.

The present study reports the effect of different seasons on polyphenol content and antioxidant potential of ethanolic, methanolic, ethyl acetate, and aqueous extracts of leaves, stems, and roots of Premna integrifolia. Ethyl acetate extract of leaves (EAEPI) collected in the rainy season showed potent antioxidant activity with highest total phenol (74.33 ± 2.26 µg/mg, gallic acid equivalent), and flavonoid (98.83 ± 0.26 µg/mg, rutin equivalent) content.

Heavy metals are ubiquitously present in nature, including soil, water, and thus in plants, thereby causing a potential health risk. This study has investigated the role and efficiency of the chickpea metallothionein 1 (MT1) gene against the major toxic heavy metals, i.e., As [As(III) and As(V)], Cr(VI), and Cd toxicity. MT1 over-expressing transgenic lines had reduced As(V) and Cr(VI) accumulation, whereas Cd accumulation was enhanced in the L3 line.

Starch can accumulate in both actively growing vegetative fronds and over-wintering propagules, or turions of duckweeds, small floating aquatic plants belonging to the family of the Lemnaceae. The starch synthesizing potential of 36 duckweed species varies enormously, and the starch contents actually occurring in the duckweed tissues are determined by growth conditions, various types of stress and the action of growth regulators.

To increase food production, prevalent agricultural malpractices such as intensive use of fertilizers and pesticides have led to degradation of the ecosystem. In this situation, there is a need to encourage eco-friendly and sustainable methods for improving crop production under ever increasing abiotic stress conditions. One such method can be through use of arbuscular mycorrhizal fungi (AMF or AM fungi). Soil microorganisms such as AMF serve as a link between plants and the soil resources.

Contamination of agricultural soil by chromium (Cr) is a serious menace to environmental safety and global food security. Although potential of salicylic acid (SA) in mitigating heavy metal (HM) toxicity in plants is well recognized, detailed physiological mechanisms behind such beneficial effects under Cr-stress in tomato (Solanum lycopersicum L.) plant are far from being completely unravelled.

In the present study, the potential role of hydrogen peroxide (H2O2) and nitric oxide (NO) has been well recorded in the induction of cadmium (Cd) stress tolerance in cyanobacteria. In this regard, H2O2 and SNP (sodium nitroprusside, NO donor), were applied to Nostoc muscorum and Anabaena sp. exposed to Cd (6 µM) stress, to analyze different physiological and biochemical parameters.

Rapid commercialization, industrialization and the use of nanotechnology has led to an increase in the distribution of nanoparticles (NPs) in the environment. The most common metal oxide NPs which is present within products is Titanium dioxide (TiO2). TiO2 NPs have photocatalytic nature and can affect plant growth.

Plant growth-promoting rhizobacteria (PGPR) represent a set of microorganisms that play significant role in improving plant growth and controlling the phytopathogens. Unpredictable performance after the application of PGPR has been observed when these were shifted from in-vitro to in-vivo conditions due to the prevalence of various abiotic stress conditions. During growing period, the potato crop is subjected to a combination of biotic and abiotic stresses.

Salinity is a major abiotic stress that negatively impacts plant health and soil microbiota. ACC (1-aminocyclopropane carboxylic acid) deaminase producing microorganisms act as natural stress busters that protect plants from different kinds of stresses. The study focused on the isolation of potent, indigenous, multi-trait ACC deaminase producers.

Salinity is a significant constraint for plant survival and productivity. Therefore, an immediate solution to this problem is sought to meet the human population's food demands. Recently, Menadione sodium bisulphite (MSB) has emerged as a significant regulator of plant defense response under abiotic stress. Studies on MSB are scarce, and a few reports on salinity (Arabidopsis and okra) and cadmium stress (okra) are present in the literature.

Cadmium (Cd) is a toxic metal and an environmental pollutant that significantly reduces plant growth and productivity. Proper management can ameliorate dysfunction and improve the plant growth and productivity exposed to Cd. Therefore, the present study was conducted to explore the protective role of the fungicides tebuconazole (TEB) and trifloxystrobin (TRI) in helping wheat (Triticum aestivum L. cv. Norin 61) seedlings to tolerate Cd.

Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. Nevertheless, the interaction between rice (Oryza sativa L.) plants and the molecular cell dynamics induced by lead-methyl jasmonate (MJ) remains unknown. Here, plants were hydroponically exposed to Pb (150 and 300 µM) alone or in combination with 0.5 and 1 µM MJ.

Cadmium (Cd) in soil–plant system can abridge plant growth by initiating alterations in root zones. Hydroponics and rhizoboxes are useful techniques to monitor plant responses against various natural and/or induced metal stresses. However, soil based studies are considered more appropriate in order to devise efficient food safety and remediation strategies.

Vigna mungo (L.) Hepper commonly known as blackgram is an important legume crop with good quality dietary proteins and vitamins. Low production of blackgram in the chromium rich soil of Odisha is a serious concern against its demand. Chromium (VI) was tested on V. mungo var. B3-8-8 at 100, 150, 200, 250 and 300 µM concentration on growth, anti-oxidative enzymes and chromium content at 15, 30 and 45 d of treatments.