Objective 1: Design, place, and assess conservation practices for improved water quality and environmental benefits. Sub-objectives: 1.1: Develop and evaluate practices for reducing surface water contaminants in artificially drained landscapes; 1.2: Evaluate perennial systems to reduce runoff, sediment, and phosphorus (P) losses; and 1.3: Increase the efficacy of the Agricultural Conservation Planning Framework (ACPF) toolbox as an approach to conservation planning for improved water quality within Midwest watersheds. Objective 2: As part of the Long-Term Agroecosystem Research (LTAR) network, and in concert with similar long-term, land-based research infrastructure in the Upper Mississippi River Basin Region, use the Upper Mississippi River Basin Experimental Watersheds LTAR site to improve the observational capabilities and data accessibility of the LTAR network and support research to sustain or enhance agricultural production and environmental quality in agroecosystems characteristic of the region. Research and data collection are planned and implemented based on the LTAR site application and in accordance with the responsibilities outlined in the LTAR Shared Research Strategy, a living document that serves as a roadmap for LTAR implementation. Participation in the LTAR network includes research and data management in support of the ARS Greenhouse gas Reduction through Agricultural Carbon Enhancement network (GRACEnet) and/or Livestock GRACEnet projects. Objective 3: Quantify the effects of landscape attributes and management practices on the fate, transformation, and transport of antibiotics, antibiotic-resistant bacteria and other emerging contaminants in surface runoff, drainage water, and streams in agricultural watersheds.