Antimicrobial-Resistance Genetics of "Emerging" Salmonella Enterica Serovar Javiana Phenotypes Involved in Clinical and Food-Related Outbreaks

<p>1) Determine the intrinsic resistance of Salmonella Javiana isolates to multiple antimicrobials by the SensiTitre antimicrobial-susceptibility testing protocol using the Clinical and Laboratory Standards Institute (CLSI) guidelines.</p>

<p>2) Determine the variation in genetic clonality among the drug-resistance genotypes by fingerprinting the bacteria using the CDC's PulseNet pulsed-field gel electrophoresis (PFGE) protocol.</p>

<p>3) Identify the genes in the multiple antibiotic region (MAR) of the Salmonella Genomic Island (SGI)-class 1 integron gene cassettes in the resistant phenotypes.</p>

<p>4) Detect antimicrobial-resistance genes in select multidrug resistant Javiana isolates by PCR-based and microarray-biochip methodologies.</p>