Antimicrobial Resistance in Swine Given 5 In-Feed Antibiotic Regimens

Objective

<OL> <LI> Determine the antimicrobial resistance patterns of Salmonella, E. coli, Enterococci and Campylobacter isolates obtained from finisher swine fed three different antibiotic feeding regimens <LI>Determine the molecular relatedness between isolates <LI>Characterize the resistance genes within these isolates.

More information

Approach: 1) Antimicrobial resistance in Salmonella, E. coli, and Enteoccocci will be determined using a semi-automated broth microdilution sensititre system (TREK Diagnostics, Inc.) and custom designed antimicrobial plates as used in the NARMS program. The E-test will be used to determine resistance in Campylobacter. 2) PFGE, and sequencing will be used to determine the molecular relatedness between isolates. 3) Microarrays, hybirdization, sequencing, plasmid analysis, and PCR will be used to characterize the resistance genes.

Investigators

Cray, Paula

Institution

USDA - Agricultural Research Service

Start date

2003

End date

2005

Funding Source

Agricultural Research Service

Project number

6612-32000-035-05R

Accession number

407824