An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Colonization Characteristics of Bovine Recto-Anal Junction Tissue by Escherichia Coli O157:H7

Objective

To determine fecal shedding dynamics of E. coli O157:H7 isolates that differ in their ability to cause disease in humans.

More information

Approach: Two groups of E. coli O157:H7 have been identified by single nucleotide polymorphisms that associate with the ability of this organism to cause disease in humans and their ability to adhere to epithelial cells in culture. This experiment addresses the legitimacy of the cell culture adherence results in the lab (in vitro) compared to real life (in vivo) conditions. To accomplish this, the recto-anal junction area in cattle will be inoculated with E. coli O157:H7 isolates from the two groups. Fecal samples will be cultured to determine the number of E. coli O157:H7 shed and length of shedding time.

Investigators
Wheeler, Tommy Lee
Institution
University of Idaho
USDA - Agricultural Research Service
Start date
2007
End date
2008
Project number
5438-32000-028-05S
Accession number
412406
Categories