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National Agricultural Library
U.S. Department of Agriculture

COMPARISON OF CRISPR-INDUCED MUTATIONS AT DUPLICATE LOCI, INCLUDING CHANGES THAT ARE ON-TARGET, NON-TARGET, AND UNINTENDED

Objective

Objective 1.Use long-read DNA sequencing to characterize duplicate genes in two soybean experimental lines.Objective 2.Characterize CRISPR modifications at duplicate genes that vary in linkage, copy number, sequence divergence, and phenotypic effect.Objective 3.Use long-read DNA sequencing and phenotypic screening to identify potential off-target or unintended changes induced by multiple CRISPR edits.

Investigators
Morrell, P.; Stupar, RO, .
Institution
UNIV OF MINNESOTA
Start date
2023
End date
2026
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
MIN-13-G43
Accession number
1031365
https://portal.nifa.usda.gov/web/crisprojectpages/1031365.html