To clone the gene from apple peel tissue that encodes alpha-farnesene synthase and express the cDNA in transformed E. coli or yeast cell lines to obtain functional, purified enzyme for biochemical studies. To determine whether in vivo oxidation of alpha-farnesene in apple peel is enzymatic, and if so, to isolate and purify the oxidase enzyme, and subsequently clone the gene that encodes the enzyme.
CONTROL OF SUPERFICIAL SCALD IN APPLES WITHOUT POSTHARVEST CHEMICAL TREATMENT
Objective
Investigators
Whitaker B D
Institution
USDA - Agricultural Research Service
Start date
2000
End date
2004
Funding Source
Project number
1275-43000-009-01R
Accession number
403233
Categories
Commodities