Specific objectives:-Test the hypothesis that tomato CDEF enzymes act as cutin hydrolases through characterization of: i) their activities in vitro; ii) semi-in vivo bioassays; and iii) cutin matrices that are deposited in planta, and determine their contributions to fruit transpiration and cuticle polymer modification.- Establish whether different CUS enzymes catalyze the formation of structurally distinct cutin polymers and show coordinated activities with CDEF enzymes.- Identify key structural features of CUS and CDEF proteins that define their modes of action as acyltransferases and acylhydrolases, respectively.- Characterize a newly identified cuticle 'self-sealing' mechanism.
CULTIVAR DEVELOPMENT: ACCELERATED INTROGRESSION OF SYNTHETIC HEXAPLOID DERIVED DIVERSITY INTO AN APPLIED HARD WINTER WHEAT BREEDING PROGRAM
Objective
Investigators
Rose, J.
Institution
Cornell University
Start date
2021
End date
2024
Funding Source
Project number
NYC-184521
Accession number
1025394
Commodities