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CULTIVAR DEVELOPMENT: ACCELERATED INTROGRESSION OF SYNTHETIC HEXAPLOID DERIVED DIVERSITY INTO AN APPLIED HARD WINTER WHEAT BREEDING PROGRAM

Objective

Specific objectives:-Test the hypothesis that tomato CDEF enzymes act as cutin hydrolases through characterization of: i) their activities in vitro; ii) semi-in vivo bioassays; and iii) cutin matrices that are deposited in planta, and determine their contributions to fruit transpiration and cuticle polymer modification.- Establish whether different CUS enzymes catalyze the formation of structurally distinct cutin polymers and show coordinated activities with CDEF enzymes.- Identify key structural features of CUS and CDEF proteins that define their modes of action as acyltransferases and acylhydrolases, respectively.- Characterize a newly identified cuticle 'self-sealing' mechanism.

Investigators
Rose, J.
Institution
Cornell University
Start date
2021
End date
2024
Project number
NYC-184521
Accession number
1025394
Commodities