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Detection of Cytolethal Distending toxin (cdtB), pltA and pltB Homologs of Components of the Pertussis Toxin Genes by PCR and Studies on Functionality of cdtB in Non-Typhoidal Salmonella spp.



<li>Develop a duplex real-time, quantitative polymerase chain reaction (qPCR) to detect Salmonella and antibioticresistance gene markers simultaneously from food samples.</li>
<li>Validation of qPCR method at FDA, ORA laboratories.</li>
<li>Characterize plasmids, virulence genes, and integrons, for their role in virulence in multidrugresistant Salmonella strains isolates from food.</li>


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Responsible NCTR Division: Microbiology

Khan, Ashraf
DHHS/FDA - National Center for Toxicological Research
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