Detection of Cytolethal Distending toxin (cdtB), pltA and pltB Homologs of Components of the Pertussis Toxin Genes by PCR and Studies on Functionality of cdtB in Non-Typhoidal Salmonella spp.

Objective

<ol>
<li>Develop a duplex real-time, quantitative polymerase chain reaction (qPCR) to detect Salmonella and antibioticresistance gene markers simultaneously from food samples.</li>
<li>Validation of qPCR method at FDA, ORA laboratories.</li>
<li>Characterize plasmids, virulence genes, and integrons, for their role in virulence in multidrugresistant Salmonella strains isolates from food.</li>
</ol>

More information

Responsible NCTR Division: Microbiology

Investigators

Khan, Ashraf

Institution

DHHS/FDA - National Center for Toxicological Research

Start date

2015

End date

2016

Funding Source

Nat'l. Center For Toxicological Research

Project number

E0739601