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<p>The goal of this project is to develop a novel non-invasive method using MRI to improve the understanding of the internalization of foodborne pathogens in produce. Once the internalization mechanism is better elucidated in a research setting, developments of effective pre-harvest interventions to reduce internalized contamination could be achieved, thus meeting USDA/NIFA goal to reduce foodborne illness and deaths through a safer food supply and to improve food safety for all Americans.</p>
<p>Bacterial ferritins will be used as report agents in MRI. A comprehensive screening of suitable strains of Shiga-toxin producing Escherichia coli, Salmonella spp., and Listeria monocytogenes that have an ability to accumulate high levels of iron in cells will be conducted. Bacterial ferritin-expressing strains will also be created using genetic modification. The ferritin entry-clone plasmids pENTR-bfr, pENTR-ftn, and pENTR-fri will be constructed. Additionally, IONPs labeled strains will be developed.Using the established ferritin-expressing strains, a MRI method will be established for internalization detection. The internalization mechanism of pathogens in various crop varieties, such as root and leafy vegetables and fruits, during preharvest growth and postharvest processing stages will be studied.<p>