This research project seeks to develop biomarkers that are predictive of subsequent tumour formation by non-genotoxic chemicals.
<p>Three different approaches are being employed to identify novel biomarkers: i) Quantitative analysis of gene expression, ii) Effects on the p53 pathway including changes in phosphorylation status, iii) Proteomic analysis by 2-dimensional gel electrophoresis to examine global changes in protein expression.
A large number of food and environmental chemicals have been shown to produce tumours in experimental animals. Such chemicals can be broadly divided into genotoxic (substances that damage DNA) and non-genotoxic agents.
<p>While screening tests are available for DNA reactive (genotoxic) agents, non-genotoxic carcinogens are only normally identified by performing chronic rodent bioassay studies.
<p>The objective of this project is to develop biomarkers that are predictive of subsequent tumour formation by non-genotoxic chemicals in experimental animals.
<p>Such biomarkers could be employed in conjunction with biomarkers of exposure, to provide a better assessment of the hazard of food chemicals to humans.
The final report, "<a href="http://www.foodbase.org.uk/results.php?f_report_id=356" target="_new">Development of Biomarkers Based on Cell Cycling and Proliferation to Facilitate the Risk Assessment of Food Chemicals</A>" is available at Foodbase, an open access repository of the <acronym title="Food Standards Agency"> FSA</acronym>.
<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/" target="_blank">Food Standards Agency Research webpage</a>.