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National Agricultural Library
U.S. Department of Agriculture
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  4. The Development of a Genotypic Selection Assay and Analysis of the Age-Specific Patterns of Mutant Accumulation

The Development of a Genotypic Selection Assay and Analysis of the Age-Specific Patterns of Mutant Accumulation

Objective

To develop a genotypic selection assay (GSA) allowing a direct measurement of mutant frequencies and molecular analysis of mutation in any non-polymorphic endogenous sequence and in any tissue; 2. To determine the spontaneous mutant frequencies (MFs) and age-associated accumulation rats (Ars) in highly (Exon 3) and poorly (Exon 4) mutable regions of Hprt coding sequence in the Hprt lymphocyte mutation assay; 4. To compare the in vivo persistence of elevated MFs in Hprt exons 3 and 4 induced after exposure to ENU.

Investigators
Aidoo, Anane
Institution
DHHS/FDA - National Center for Toxicological Research
Funding Source
Nat'l. Center For Toxicological Research
Project number
E0706301