DIRECT BINDING OF NON-SPECIFIC CYTOTOXIC CELLS (NCCS) TO BACTERIA IS AN IMPORTANT INNATE IMMUNE MECHANISM IN CATFISH

Determine the basic mechanisms required for direct bacterial binding and killing by catfish NCCs.Is complement mediated direct binding and killing used by NCCsIs antibody dependent cellular cytotoxicity (ADCC) another mechanism involved in direct bacterial binding by catfish NCCs?Are accessary cells required for direct NCC bacteria binding to occurDoes direct binding occur with bacteria other than E. ictaluri?Do these mechanisms occur in hybrid catfish?Determine if direct NCC:bacteria binding and formation of the immune synapse involves cell membrane changes and cytoskeletal rearrangement.How does the NCC membrane directly bind and engage bacteria?What are the cytoskeletal characteristics of direct NCC:bacteria binding?Do hybrid catfish NCCs do the same thing?