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DISSECTING MOLECULAR PLANT-MICROBIOME INTERACTIONS FOR IMPROVED DROUGHT TOLERANCE IN MAIZE

Objective

Objective 1: Characterize the response of the maize root microbiome to droughtObjective 1a Select and collect focal soil microbiomesObjective 1b Set up factorial drought experiment using field soil inoculaObjective 1c Quantify plant performance and root microbiomesObjective 1d Analyze data and test hypothesesObjective 1e Culture root endophytes from most drought-resistant plantsObjective 2: Select, test, and sequence root bacteria to add to SynComObjective 2a Identify isolates using Sanger sequencing and colony morphologyObjective 2b Assess root colonization efficiency and reproducibility using expanded SynComObjective 2c Sequence genomes of select organismsObjective 2d Screen genome-sequenced isolates for ability to improve drought toleranceObjective 3: Dissect functional interactions that confer drought toleranceObjective 3a Identify taxon-specific microbial genes for in planta growthObjective 3b Test the effect of keystone species on microbial and plant protein expressionObjective 3c Characterize effects of drought treatment and host genotype on root microbiome protein expressionObjective 3d Identify molecular mechanisms of drought-tolerance improvement by root microbes

Investigators
Kleiner, M.; Wagner, Ma.
Institution
North Carolina State University
Start date
2022
End date
2024
Project number
NC09931
Accession number
1028007