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Effects of Dietary Restriction on the Post-Initiation Stages in Aflatoxin B1(AFB1)-Induced Carcinogenesis on Male F344 Rats Fed Methyl-Deficient Diets


To study the interactions of dietary restriction (DR) and methyl deficiency (MD) onthe alterations of hepatic oxidative DNA damages, DNA methylation, cellproliferation,oncogene and tumor suppressor gene mutation, preneoplastic fociformation and tumor incidence during the post-initiation stages of AFB1-inducedcarcinogenesis in male F344 rats. The results of these studies will:
<li> Test the hypothesis that DR may be an antagonist to the promotional effect of MDin the AFB1-induced carcinogenesis.</li>
<li> Evaluate the correlations between the effects of DR and MD on the formation ofAFB1-induced preneoplastic foci and tumors and various biomarkers during thepost-initiation stages of carcinogenesis.</li></ol>

More information

FY 2000 Accomplishments:A manuscript describing relationships between methyl deficiency, dietary restriction,hepatic cell proliferation, and telomerase activity was published.FY 2001 Plans:Assay oxidative damage in liver DNA from rats fed methyl-deficient diets and deter-minewhether or not oxidative damage can be reduced by dietary restriction.</p>

Chou, Ming
DHHS/FDA - National Center for Toxicological Research
Project number