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The Evaluation of the Genotoxicity and Concentration-Response Relationships of the Azo Dyes Sudan 1 and Para Red


This research project seeks to evaluate the genotoxicity of azo dyes and to investigate whether the genotoxicity occurs via a threshold mechanism

<p>This project will employ methodologies developed as part of another FSA research project (T01029 – The evaluation of the existence of thresholds of activity of genotoxic substances).

<p>Using cultured human lymphoblastoid cells, azo dyes will be assessed for their ability to induce gene mutations (at TK and HPRT loci) and chromosomal structural and numerical changes.

<p>Protocols will also be modified to allow assessment of effects of potential biotransformation of the azo dyes.

<p>Emphasis will be placed on fully investigating the shape of the genotoxicity dose-response relationship in order to establish if this effect exhibits a threshold.

<p>Studies will also investigate potential interactions of combined exposure to azo dyes and other genotoxins with known mechanisms of action.

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The discovery of non-permitted azo dyes in food has highlighted the paucity of toxicological data relating to this class of chemicals.

<p>In the first instance, this project will determine whether two azo dyes Sudan 1 and Para Red induce gene or chromosome mutations and, if so, particular emphasis will be placed on establishing the shape of the dose-response relationships for these effects.

<p>Find more about this project and other FSA food safety-related projects at the <a href="; target="_blank">Food Standards Agency Research webpage</a>.

University of Wales - Swansea
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