This research project seeks to evaluate the genotoxicity of azo dyes and to investigate whether the genotoxicity occurs via a threshold mechanism
<p>This project will employ methodologies developed as part of another FSA research project (T01029 – The evaluation of the existence of thresholds of activity of genotoxic substances).
<p>Using cultured human lymphoblastoid cells, azo dyes will be assessed for their ability to induce gene mutations (at TK and HPRT loci) and chromosomal structural and numerical changes.
<p>Protocols will also be modified to allow assessment of effects of potential biotransformation of the azo dyes.
<p>Emphasis will be placed on fully investigating the shape of the genotoxicity dose-response relationship in order to establish if this effect exhibits a threshold.
<p>Studies will also investigate potential interactions of combined exposure to azo dyes and other genotoxins with known mechanisms of action.
The discovery of non-permitted azo dyes in food has highlighted the paucity of toxicological data relating to this class of chemicals.
<p>In the first instance, this project will determine whether two azo dyes Sudan 1 and Para Red induce gene or chromosome mutations and, if so, particular emphasis will be placed on establishing the shape of the dose-response relationships for these effects.
<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/" target="_blank">Food Standards Agency Research webpage</a>.