An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

National Agricultural Library
U.S. Department of Agriculture
  1. Home
  2. Research Tools
  3. Food Safety Research Projects Database
  4. Formation of Biofilms by E. coli O157:H7

Formation of Biofilms by E. coli O157:H7

Objective

The objective of this cooperative research project is to test the hypothesis that E. coli O157:H7 forms biofilms on colonic epithelial surfaces in the ruminant intestinal tract.

More information

The hypothesis will be tested by completing the following objectives: 1) Compare gene expression profiles of E. coli O157:H7 during planktonic growth, biofilm formation on an abiotic surface and growth on a biotic surface of epithelial cells; 2) Determine if biofilm mutants differ from wild-type E. coli O157:H7 in their interactions with 3-dimensional T-84 colonic epithelial cells and their gene expression profiles; 3) Determine if E. coli O157:H7 strains associated with human disease (EHEC) have a greater capacity to form biofilms than ruminant isolates.

Investigators
Sharma, Vijay
Institution
Iowa State University
Start date
2004
End date
2006
Funding Source
Agricultural Research Service
Project number
3625-32000-051-07S
Accession number
408994
Categories
Escherichia coli
Bacterial Pathogens