The overall goal of this research project is to enhance food safety by developing methods to reduce levels of trichothecenes and other mycotoxins that occur in grain crops as a result of infection by Fusarium graminearum (sexual stage, Gibberella zeae) and related trichothecene-producing species of Fusarium. FHB is a world-wide threat to grain producers and consumers, due to the loss in yield and to the presence of trichothecenes and other mycotoxins in the grain. As the worldÂ¿s population continues to increase, the need to reduce mycotoxins in grain will increase. Development of methods to reduce mycotoxin contamination in grain will be enhanced through elucidation of the molecular genetic mechanisms that control mycotoxin production in F. graminearum and related fusaria, that control plant-fungal interactions, and that detoxify or otherwise modify mycotoxins. The objectives and proposed research are as follows: Objective 1: Identify and characterize mycotoxin detoxification genes as a mechanism to reduce/eliminate the toxins in grain-based food and feed; Objective 2: Determine the genetic bases and ecological significance of variation in types of trichothecene mycotoxins produced by Fusarium; Objective 3: Identify and characterize plant genes that affect biosynthesis of trichothecenes and other mycotoxins produced by Fusarium.