The objective is:<ol><li>To establish a database for genomics data.
<li> To relate the changes in gene expression to in vitro genotoxicity measures that are utilized in hazard assessment
A 'working group' at the NCTR has been established in order to conduct the experiments for the ILSI project. Initial studies on the extraction of RNA and DNA from TK6 cells have been conducted. A dose-range-finding study is nearing completion with the initial chemical, benzo(a)pyrene diol epoxide (BPDE), and experimentation will begin in FY 2001.
Two cell strains will be exposed to known carcinogens, the mutant frequency at the Thymidine kinase locus will be measured, and the formation of specific DNA adducts will be quantified. RNA will be isolated and sent to CDER for gene expression analysis. The data generated from this project will be entered into the ILSI database for further analysis.