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National Agricultural Library
U.S. Department of Agriculture
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Laboratory Detection of Toxoplasma in the Environment

Objective

To develop test for the detection of Toxplasma gondii in environmental water supplies.
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Toxoplasma gondii oocysts will be obtained from feces of experimentally infected cats, purified by sucrose-gradients. Oocysts will be sporulated in 2% sulfuric acid as preservative and then stored at 4 C until used. Known numbers of oocysts will be mixed with water samples and then sensitivity of various methods (bioassay, PCR, monoclonal antibodies) will be tested.

Investigators
Dubey, Jitender
Institution
USDA - Agricultural Research Service
Start date
2002
End date
2005
Funding Source
Agricultural Research Service
Project number
1265-32000-066-13R
Accession number
405983
Categories
Prevention and Control