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Laboratory Detection of Toxoplasma in the Environment


To develop test for the detection of Toxplasma gondii in environmental water supplies.
Toxoplasma gondii oocysts will be obtained from feces of experimentally infected cats, purified by sucrose-gradients. Oocysts will be sporulated in 2% sulfuric acid as preservative and then stored at 4 C until used. Known numbers of oocysts will be mixed with water samples and then sensitivity of various methods (bioassay, PCR, monoclonal antibodies) will be tested.

Dubey, Jitender
USDA - Agricultural Research Service
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