<p>This workpackage aims to study the distribution and characteristics of SGI1 in enteric bacteria (a.o. Salmonella, Shigella, E. coli) in a large collection of animal and human isolates. The result will be a database of strains harbouring (parts of) SGI1. This database will be a basis for future virulotyping, and risk assessment of isolates harbouring SGI1.</p>
Progress: Phenotypic and molecular methods for selection of isolates suspected of harboring SGI1 have been identified.
With the aid of the phenotypic selection criteria, ten out of thirteen participating laboratories have contributed to a collection of 399 isolates (of Salmonella, E. coli and Shigella). <P>
SGI-1 was detected in the following Salmonella serovars: Agona, Albany, Derby, Java, Kentucky, Newport and Paratyphi B. SGI-1-RP was detected in Enteritidis, Senftenberg and Typhimurium. In one isolate of S. Typhimurium, only the left junction of the Salmonella genomic island-1 was detected. The presence of a SGI-1 was not detected in E. coli or Shigella. <P>
Class 1 integrons, which may be associated SGI1, were detected in E. coli, Shigella sonnei and in 24 out of 28 Salmonella serovars tested.
Methods for the molecular characterisation of this gene cluster have been discussed and described. A selection of SGI-1 positive isolates will be made to validate molecular characterization approaches. For characterization only, PCRs aimed at a list of ten different genes specific for SGI-1 will be used.