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Pilot Study on the Application of Whole Genome Sequencing Approach for the Molecular Typing of Foodborne Bacterial Pathogens


<OL> <LI> To evaluate the ability of PPC to reduce fecal shedding of E. coli O157:H7 in lambs challenged with E. coli O157:H7
<LI> To determine the role of PPC tannin in reducing E coli O157:H7 fecal shedding.
<LI> To determine their kill site of E. coli O157:H7 in digestive tract of ruminant (i.e rumen or large intestine). </OL>
In vitro experiments will also be conducted to assess the effects of PPC and PPC tannins on ruminal fermentation and the mechanism by which PPC tannin affects ruminal microbial populations.

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Expected Benefits:<BR>

By assessing the potential of PPC and PPC tannin for reducing E. coli O157:H7 in experimentally inoculated animals, this study may identify an economically feasible protocol by which livestock producers can implement a pre-harvest strategy to control E. coli O157:H7 in cattle. Proactive approaches to controlling E. coli O157:H7 in beef cattle contribute significantly towards minimizing the risk posed by this zoonotic pathogen to the Canadian food supply.
Recommendation of an economically feasible dietary intervention strategy which will control E. coli O157:H7 in ruminant will be instrumental in reducing numbers of E. coli O157-positive cattle entering packing plants and minimizing the incidence of foodborne illness associated with this pathogen

Wang, Yuxi
Agriculture and Agri-Food Canada
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