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Quantitative Evaluation of Selective Factors Impacting the Development, Amplification, Persistence, and Transfer of Resistance in Pig Flora


To quantitatively model the effects of therapeutic and sub-therapeutic tylosin administration on the development, amplification, persistence, and transfer of genetic elements conferring macrolide resistance within mixed populations of commensal and pathogenic gut bacteria grown in continuous-flow culture.

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Approach: Commensal and foodborne pathogenic gut bacteria will be grown under conditions of sub-therapeutic and therapeutic tylosin use. Samples removed from the populations will be tested using cultural and molecular techniques to determine phenotypic and genotypic antimicrobial resistance characteristics.

Anderson, Robin
USDA - Agricultural Research Service
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